Recombinant Human IL-3 (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-8039-U002 | 2 µg | $61.00 | ||
| 21-8039-U010 | 10 µg | $150.00 | ||
| 21-8039-U100 | 100 µg | $510.00 |
Technical Data Sheet
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IL-3 is a major hematopoietic growth factor produced by basophils and activated T cells and drives the differentiation of hematopoietic stem cells into myeloid lineages precursors. In conjunction with IL-7, IL-3 will induce lymphoid precursor cell differentiation. Together with other cytokines including EPO, GM-CSF, and IL-6 in an immune response, IL-3 will support growth and differentiation of T cells from the bone marrow.
IL-3 is a major hematopoietic growth factor produced by basophils and activated T cells and drives the differentiation of hematopoietic stem cells into myeloid lineages precursors. In conjunction with IL-7, IL-3 will induce lymphoid precursor cell differentiation. Together with other cytokines including EPO, GM-CSF, and IL-6 in an immune response, IL-3 will support growth and differentiation of T cells from the bone marrow.
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Product Details
| Name | Recombinant Human IL-3 (Carrier-free) |
|---|---|
| Cat. No. | 21-8039 |
| Alternative Names | MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor |
| Amino Acid Sequence | APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1x 107 units/mg. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant Human IL-3 is a 15.0 kDa protein of 133 amino acid residues. |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | E. coli |
| Reactivity | Human |
| Research Areas | AIDS/HIV; Cancer; Cell Culture; Immune System; Proliferation; Stem Cells & Differentiation |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.