Recombinant Human IL-27 (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-8279-U002 | 2 µg | $79.00 | ||
| 21-8279-U010 | 10 µg | $269.00 |
Technical Data Sheet
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IL-27 is formed by the association of an IL-27-p28 subunit (also known as IL-30) with the Epstein-Barr Virus (EBV)-induced Gene 3 (EBI3) subunit (also known as IL-27B). It belongs to the IL-12 family of heterodimeric cytokines. IL-27 is expressed by antigen presenting cells (APCs) at sites of inflammation in cytokine-driven autoimmune/inflammatory diseases, and plays a role in promoting Th1 polarization. IL-27 also has anti-inflammatory functions that seem to limit T and NK-T cell activity. It binds to a receptor complex composed of IL-27R (also known as TCCR/WSX-1) and gp130, a receptor shared by IL-6.
IL-27 is formed by the association of an IL-27-p28 subunit (also known as IL-30) with the Epstein-Barr Virus (EBV)-induced Gene 3 (EBI3) subunit (also known as IL-27B). It belongs to the IL-12 family of heterodimeric cytokines. IL-27 is expressed by antigen presenting cells (APCs) at sites of inflammation in cytokine-driven autoimmune/inflammatory diseases, and plays a role in promoting Th1 polarization. IL-27 also has anti-inflammatory functions that seem to limit T and NK-T cell activity. It binds to a receptor complex composed of IL-27R (also known as TCCR/WSX-1) and gp130, a receptor shared by IL-6.
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Product Details
| Name | Recombinant Human IL-27 (Carrier-free) |
|---|---|
| Cat. No. | 21-8279 |
| Alternative Names | Interleukin-27 subunit alpha, IL-27-A, IL27A, Interleukin-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI3, p28, Interleukin-30, IL-30 |
| Amino Acid Sequence | FPRPPGRPQL SLQELRREFT VSLHLARKLL SEVRGQAHRF AESHLPGVNL YLLPLGEQLP DVSLTFQAWR RLSDPERLCF ISTTLQPFHA LLGGLGTQGR WTNMERMQLW AMRLDLRDLQ RHLRFQVLAA GFNLPEEEEE EEEEEEEERK GLLPGALGSA LQGPAQVSWP QLLSTYRLLH SLELVLSRAV RELLLLSKAG HSVWPLGFPT LSPQPRKGPP AALTLPRVQC RA |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 range is 400-500 ng/ml. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant human IL-27 produced from HEK-293 cells is a non-disulfide-linked, heterodimeric protein composed of a 209 amino acid length EBI3 subunit and a 215 amino acid length IL-27-p28 subunit, for a total sequence length of 424 amino acid residues. Th |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 90 |
| Source | HEK293 cells |
| Reactivity | Human |
| Research Areas | Immune System, Inflammation, Proliferation |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.