Recombinant Human IL-12 p70 (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-8129-U002 | 2 µg | $69.00 | ||
| 21-8129-U010 | 10 µg | $195.00 | ||
| 21-8129-U100 | 100 µg | $1,140.00 |
Technical Data Sheet
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Interleukin-12 (IL-12) is a heterodimeric pro-inflammatory cytokine composed of IL-12A (p35) and IL-12B (p40) acting on predominantly T cells and NK cells. IL-12 is a strong inflammatory mediator produced by dendritic cells (DCs) and phagocytes upon exposure to pathogens also indicating its bridging of innate and adaptive immune responses. IL-12 appears to be important in T cell (CD4 and CD8) maturation and NK cell activity. IL-12 induces IFN-gamma and TNF-alpha from both NK cells and T cells while diminishing IL-4 mediated suppression of IFN-gamma.
Interleukin-12 (IL-12) is a heterodimeric pro-inflammatory cytokine composed of IL-12A (p35) and IL-12B (p40) acting on predominantly T cells and NK cells. IL-12 is a strong inflammatory mediator produced by dendritic cells (DCs) and phagocytes upon exposure to pathogens also indicating its bridging of innate and adaptive immune responses. IL-12 appears to be important in T cell (CD4 and CD8) maturation and NK cell activity. IL-12 induces IFN-gamma and TNF-alpha from both NK cells and T cells while diminishing IL-4 mediated suppression of IFN-gamma.
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Product Details
| Name | Recombinant Human IL-12 p70 (Carrier-free) |
|---|---|
| Cat. No. | 21-8129 |
| Alternative Names | NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF |
| Amino Acid Sequence | p35 Subunit: RNLPVA TPDPGMFPCL HHSQNLLRAV SNMLQKARQT LEFYPCTSEE IDHEDITKDK TSTVEACLPL ELTKNESCLN SRETSFITNG SCLASRKTSF MMALCLSSIY EDLKMYQVEF KTMNAKLLMD PKRQIFLDQN MLAVIDELMQ ALNFNSETVP QKSSLEEPDF YKTKIKLCIL LHAFRIRAVT IDRVMSYLNA S p40 Subunit: IWELKK DVYV |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | The ED50 was determined by the induction of IFN-g from NK cells co-stimulated with IL-18 is ? 1.0 ng/ml, corresponding to a specific activity of ≥ 1x 106 units/mg. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant Human IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues). |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | CHO cells |
| Reactivity | Human |
| Research Areas | Angiogenesis/Cardiovascular; Cancer; Immune System; Inflammation; Stem Cells & Differentiation; Allergy; Transplantation |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.