Recombinant Human IGF-II (Carrier-free)

Cat No. Size Price Quantity
21-7069-U010 10 µg $69.00
21-7069-U050 50 µg $170.00
21-7069-U100 100 µg $260.00
21-7069-U500 500 µg $720.00
Lead time: 2-3 business days

Description

IGF-II (insulin-like growth factor II) is a member of a family of proteins involved in mediating growth and development. IGF-II expression is regulated by lactogen, is expressed in many cell and tissue types, and may have autocrine, paracrine and endocrine functions. IGF-II binds with higher affinity to IGF-R II than IGF-R I. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. As with IGF-I, association with IGF binding proteins acts to extend the half-life of IGF-II and regulates receptor interaction.
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IGF-II (insulin-like growth factor II) is a member of a family of proteins involved in mediating growth and development. IGF-II expression is regulated by lactogen, is expressed in many cell and tissue types, and may have autocrine, paracrine and endocrine functions. IGF-II binds with higher affinity to IGF-R II than IGF-R I. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. As with IGF-I, association with IGF binding proteins acts to extend the half-life of IGF-II and regulates receptor interaction.
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Product Details

Name Recombinant Human IGF-II (Carrier-free)
Cat. No. 21-7069
Alternative Names Insulin-like Growth Factor-I, Somatamedin C, IGF-IA
Amino Acid Sequence AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ? 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant human IGF-II is a globular protein containing 67 amino acids and 3 intra-molecular disulfide bonds, and has a predicted molecular mass of approximately 7.47 kD.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E. coli
Reactivity Human
Research Areas Angiogenesis/Cardiovascular; Bone, Skeletal, Cartilage; Diabetes / Weight Regulation; Inflammation; Proliferation; Wound Healing
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.