Recombinant Human IGF-II (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-7069-U010 | 10 µg | $69.00 | ||
| 21-7069-U050 | 50 µg | $170.00 | ||
| 21-7069-U100 | 100 µg | $260.00 | ||
| 21-7069-U500 | 500 µg | $720.00 |
Lead time: 2-3 business days
Technical Data Sheet
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IGF-II (insulin-like growth factor II) is a member of a family of proteins involved in mediating growth and development. IGF-II expression is regulated by lactogen, is expressed in many cell and tissue types, and may have autocrine, paracrine and endocrine functions. IGF-II binds with higher affinity to IGF-R II than IGF-R I. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. As with IGF-I, association with IGF binding proteins acts to extend the half-life of IGF-II and regulates receptor interaction.
IGF-II (insulin-like growth factor II) is a member of a family of proteins involved in mediating growth and development. IGF-II expression is regulated by lactogen, is expressed in many cell and tissue types, and may have autocrine, paracrine and endocrine functions. IGF-II binds with higher affinity to IGF-R II than IGF-R I. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. As with IGF-I, association with IGF binding proteins acts to extend the half-life of IGF-II and regulates receptor interaction.
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Product Details
| Name | Recombinant Human IGF-II (Carrier-free) |
|---|---|
| Cat. No. | 21-7069 |
| Alternative Names | Insulin-like Growth Factor-I, Somatamedin C, IGF-IA |
| Amino Acid Sequence | AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ? 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant human IGF-II is a globular protein containing 67 amino acids and 3 intra-molecular disulfide bonds, and has a predicted molecular mass of approximately 7.47 kD. |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | E. coli |
| Reactivity | Human |
| Research Areas | Angiogenesis/Cardiovascular; Bone, Skeletal, Cartilage; Diabetes / Weight Regulation; Inflammation; Proliferation; Wound Healing |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.