Recombinant Human IFN-beta (Carrier-free)
|Name||Recombinant Human IFN-geta (Carrier-free)|
|Alternative Names||Leukocyte interferon, B cell interferon, Type I interferon, IFN-g|
|Amino Acid Sequence||MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN|
|Authenticity||Verified by N-terminal and Mass Spectrometry analyses (when applicable).|
Assay #1:Measured by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 20-30 ng/ml.
Assay #2: Determined by its ability to stimulate the prolifera
|Endotoxin Level||Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).|
|Molecular Mass||Recombinant human IFN-geta is an 20.0 kDa protein containing 166 amino acid residues. Due to glycosylation, IFNbeta has an approximate MW of ≥ 22.3 kDa based on SDS-PAGE gel and Mass Spectrometry.|
|Protein Content||Content Verified by UV Spectroscopy and/or SDS-PAGE gel.|
|Research Areas||AIDS/HIV; Cancer; Immune System; Neurobiology|
|Reconstitution||See Certificate of Analysis (COA) for lot specific reconstitution information.|
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.