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Recombinant Human HGF (Carrier-free)
  • Recombinant Human HGF (Carrier-free)

Recombinant Human HGF (Carrier-free)

Cat No. Size Price Quantity
21-8439-U002 2 µg $76.00
21-8439-U010 10 µg $175.00
21-8439-U100 100 µg $945.00
21-8439-U500 500 µg $2,795.00

Description

Hepatocyte Growth Factor (HGF), also known as Hepatopoietin A and Scatter Factor, is a dimeric molecule composed of the ?-subunit and beta-subunit, linked by a disulfide bond. It is secreted as a single inactive polypeptide and this pro-petide is cleaved by serine proteases into alpha- and beta-chains. HGF is reported to be a hepatrophic factor and acts as a growth factor for a wide variety of tissues and cell types. Its functions include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. The protein is secreted by mesenchymal cells and, after binding to the proto-oncogenic c-Met receptor, activates a tyrosine kinase signaling cascade. Hypoxia and hyperglycemia suppress HGF production.
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Hepatocyte Growth Factor (HGF), also known as Hepatopoietin A and Scatter Factor, is a dimeric molecule composed of the ?-subunit and beta-subunit, linked by a disulfide bond. It is secreted as a single inactive polypeptide and this pro-petide is cleaved by serine proteases into alpha- and beta-chains. HGF is reported to be a hepatrophic factor and acts as a growth factor for a wide variety of tissues and cell types. Its functions include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. The protein is secreted by mesenchymal cells and, after binding to the proto-oncogenic c-Met receptor, activates a tyrosine kinase signaling cascade. Hypoxia and hyperglycemia suppress HGF production.
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Product Details

Name Recombinant Human HGF (Carrier-free)
Cat. No. 21-8439
Alternative Names Scatter Factor (SF), Hepatopoietin (HPTA)
Amino Acid Sequence

alpha chain: QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH R

Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity The ED50 was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of ≥ 20.0-40.0 ng/ml.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant human HGF, under reducing conditions, has a 69-kDa alpha-chain and 34-kDa beta-chain. The alpha-chain consists of 463 amino acid residues and the beta-chain consists of ≥ 234 amino acid residues.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source (BTI-Tn-5B1-4) Hi-5 Insect cells*
Reactivity Human
Research Areas Angiogenesis/Cardiovascular; Cancer; Neurobiology; Proliferation; Stem Cells & Differentiation; Wound Healing
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.