Recombinant Human HB-EGF (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-7061-U010 | 10 µg | $69.00 | ||
| 21-7061-U050 | 50 µg | $175.00 | ||
| 21-7061-U100 | 100 µg | $295.00 | ||
| 21-7061-U500 | 500 µg | $859.00 |
Technical Data Sheet
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Human HB-EGF is a group 2 ErbB ligand and a member of the EGF family of peptide growth factors. It is able to activate both the EGF/ErbB1 and ErbB4 receptors, and EGFR binding induces both mitogenic and non-mitogenic activity. Cell types and tissues known to express this growth factor include vascular endothelial and smooth muscle cells, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. HB-EGF is required for normal cardiac valve formation and heart function, and stimulates proliferation of various cell types including smooth muscle cells, fibroblasts, epithelial cells and keratinocytes. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF.
Human HB-EGF is a group 2 ErbB ligand and a member of the EGF family of peptide growth factors. It is able to activate both the EGF/ErbB1 and ErbB4 receptors, and EGFR binding induces both mitogenic and non-mitogenic activity. Cell types and tissues known to express this growth factor include vascular endothelial and smooth muscle cells, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. HB-EGF is required for normal cardiac valve formation and heart function, and stimulates proliferation of various cell types including smooth muscle cells, fibroblasts, epithelial cells and keratinocytes. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF.
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Product Details
| Name | Recombinant Human HB-EGF (Carrier-free) |
|---|---|
| Cat. No. | 21-7061 |
| Alternative Names | Heparin Binding EGF-like growth factor, HBEGF, Diphtheria toxin receptor (DTR) |
| Amino Acid Sequence | DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECKYVKELRAPSC ICHPGYHGER CHGLSL |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ? 1.0 ng/ml, corresponding to a specific activity of ≥≥≤ 1 x 106 units/mg. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant human HB-EGF is a 9.7 kDa protein containing 86 amino acid residues, corresponding to the extra-cellular EGF-like and heparin binding domains of the full length HB-EGF protein. |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 95 |
| Source | E. coli |
| Reactivity | Human |
| Research Areas | Angiogenesis/Cardiovascular; Apoptosis; Proliferation; Wound Healing |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.