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Recombinant Human Gremlin-1 (Carrier-free)
  • Recombinant Human Gremlin-1 (Carrier-free)

Recombinant Human Gremlin-1 (Carrier-free)

Cat No. Size Price Quantity
21-7060-U010 10 µg $76.00
21-7060-U050 50 µg $175.00
21-7060-U100 100 µg $295.00
21-7060-U500 500 µg $859.00

Description

Gremlin-1 is a member of the Dan family of secreted glycoproteins. As an antagonist of BMP (binds to BMP-2, BMP-4 and BMP-7), Gremlin-1 may play a role in regulating organogenesis, body patterning, and tissue differentiation and can act as an inhibitor of monocyte chemotaxis. It is expressed at various levels in the small intestine, fetal brain, colon, ovary, prostate, pancreas and skeletal muscle. There are two isoforms for human Gremlin - isoform 1 is the standard protein, and isoform 2 has a 40 amino acid deletion in the N-terminal region. As is typical of members of the TGF-beta superfamily. this protein also contains a cysteine knot motif.
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Gremlin-1 is a member of the Dan family of secreted glycoproteins. As an antagonist of BMP (binds to BMP-2, BMP-4 and BMP-7), Gremlin-1 may play a role in regulating organogenesis, body patterning, and tissue differentiation and can act as an inhibitor of monocyte chemotaxis. It is expressed at various levels in the small intestine, fetal brain, colon, ovary, prostate, pancreas and skeletal muscle. There are two isoforms for human Gremlin - isoform 1 is the standard protein, and isoform 2 has a 40 amino acid deletion in the N-terminal region. As is typical of members of the TGF-beta superfamily. this protein also contains a cysteine knot motif.
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Product Details

Name Recombinant Human Gremlin-1 (Carrier-free)
Cat. No. 21-7060
Alternative Names CKTSF1B1, DAND2, Down-regulated in Mos-transformed cells (DRM), Increased in High Glucose protein 2 (IHG-2)
Amino Acid Sequence KKKGSQGAIP PPDKAQHNDS EQTQSPQQPG SRNRGRGQGR GTAMPGEEVL ESSQEALHVT ERKYLKRDWC KTQPLKQTIH EEGCNSRTII NRFCYGQCNS FYIPRHIRKE EGSFQSCSFC KPKKFTTMMV TLNCPELQPP TKKKRVTRVK QCRCISIDLD
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Determined by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 0.07-0.11 µg/ml.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Gremlin-1 is a 18.3 kDa protein containing 160 amino acid residues.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 90
Source CHO cells
Reactivity Human
Research Areas Bone, Skeletal, Cartilage; Cancer; Stem Cells & Differentiation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.