Recombinant Human GDF-3 (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-7059-U005 | 5 µg | $76.00 | ||
| 21-7059-U020 | 20 µg | $199.00 | ||
| 21-7059-U100 | 100 µg | $769.00 | ||
| 21-7059-U500 | 500 µg | $2,295.00 |
Technical Data Sheet
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GDF-3 is a member of the BMP family of TGF-beta superfamily proteins, and is highly homologous to GDF-9. The GDF (Growth/Differentiation Factor) group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein. With the exception of GDF-3 and -9, GDF dimers are disulfide-linked proteins. GDF-3 is a secreted protein and is expressed in undifferentiated embryonic stem (ES) cells, ossifying bone, brain, bone marrow, spleen, thymus, and adipose tissue. This protein displays inhibitory activity towards other TGF-beta superfamily members including BMPs, and participates in regulatory functions in embryogenesis. GDF3 has been shown to be induced by a high fat diet, and is associated with adipogenesis and obesity.
GDF-3 is a member of the BMP family of TGF-beta superfamily proteins, and is highly homologous to GDF-9. The GDF (Growth/Differentiation Factor) group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein. With the exception of GDF-3 and -9, GDF dimers are disulfide-linked proteins. GDF-3 is a secreted protein and is expressed in undifferentiated embryonic stem (ES) cells, ossifying bone, brain, bone marrow, spleen, thymus, and adipose tissue. This protein displays inhibitory activity towards other TGF-beta superfamily members including BMPs, and participates in regulatory functions in embryogenesis. GDF3 has been shown to be induced by a high fat diet, and is associated with adipogenesis and obesity.
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Product Details
| Name | Recombinant Human GDF-3 (Carrier-free) |
|---|---|
| Cat. No. | 21-7059 |
| Alternative Names | Growth/Differentiation Factor-3, Vg-related gene 2 (VGR-2) |
| Amino Acid Sequence | AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDE CGCG |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | Determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 100-150 ng/ml. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant human GDF-3 is a 26.0 kDa non-disulfide-linked homodimer containing two 114 amino acid polypeptide chains. |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | E. coli |
| Reactivity | Human |
| Research Areas | Diabetes / Weight Regulation; Stem Cells & Differentiation; TGF-beta Superfamily |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.