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Recombinant Human Epigen (Carrier-free)
  • Recombinant Human Epigen (Carrier-free)

Recombinant Human Epigen (Carrier-free)

Cat No. Size Price Quantity
21-7183-U005 5 µg $76.00
21-7183-U025 25 µg $175.00
21-7183-U100 100 µg $570.00
21-7183-U500 500 µg $1,545.00

Description

Epigen is a member of the EGF superfamily of proteins that signals through the ErbB receptor-1. As are other members of the EGF superfamily, human Epigen is initially synthesized as a glycosylated 14.7 kDa transmembrane-bound precursor that is proteolytically cleaved from the plasma membrane. Epigen is mitogenic for fibroblasts and epithelial cells. Although Epigen has low binding affinity to EGF receptors, it has strong mitogenic potency due to its prolonged binding presence. Expression of Epigen has been detected in multiple tissues including testis, liver and heart. Transcript and protein expression have also been reported in cancer specimens of the breast, bladder, and prostate.
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Epigen is a member of the EGF superfamily of proteins that signals through the ErbB receptor-1. As are other members of the EGF superfamily, human Epigen is initially synthesized as a glycosylated 14.7 kDa transmembrane-bound precursor that is proteolytically cleaved from the plasma membrane. Epigen is mitogenic for fibroblasts and epithelial cells. Although Epigen has low binding affinity to EGF receptors, it has strong mitogenic potency due to its prolonged binding presence. Expression of Epigen has been detected in multiple tissues including testis, liver and heart. Transcript and protein expression have also been reported in cancer specimens of the breast, bladder, and prostate.
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Product Details

Name Recombinant Human Epigen (Carrier-free)
Cat. No. 21-7183
Alternative Names EPG, Epithelial mitogen
Amino Acid Sequence AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHELEKA ICRCFTGYTG ERCEHLTLTS YA
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells. The expectedED50 for this effect is 150-300 ng/ml.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human Epigen is a 7.9 kDa monomeric protein, containing 72 amino acid residues, which comprises the EGF-homologous portion of the Epigen precursor.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source E.coli
Reactivity Human
Research Areas Cancer, Inflammation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.