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Recombinant Human DKK-3 (Carrier-free)
  • Recombinant Human DKK-3 (Carrier-free)

Recombinant Human DKK-3 (Carrier-free)

Cat No. Size Price Quantity
21-7102-U002 2 µg $69.00
21-7102-U010 10 µg $179.00
21-7102-U100 100 µg $995.00
21-7102-U500 500 µg $2,995.00

Description

The DKK (Dickkopf) family of secreted proteins includes DKK-1 through -4, and a related protein, Soggy (DKK-11). DKK-3 exhibits the most sequence homology with Soggy. It is expressed in many normal tissues including brain, spinal cord and heart. Also known as REIC (Reduced Expansion in Immortalized Cells), DKK-3 may be a negative growth regulator as expression is downregulated in a variety of cancer cell lines and tumors. Unlike other DKK members, it has been suggested that DKK-3 does not bind to LRP5 and/or LRP6.
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The DKK (Dickkopf) family of secreted proteins includes DKK-1 through -4, and a related protein, Soggy (DKK-11). DKK-3 exhibits the most sequence homology with Soggy. It is expressed in many normal tissues including brain, spinal cord and heart. Also known as REIC (Reduced Expansion in Immortalized Cells), DKK-3 may be a negative growth regulator as expression is downregulated in a variety of cancer cell lines and tumors. Unlike other DKK members, it has been suggested that DKK-3 does not bind to LRP5 and/or LRP6.
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Product Details

Name Recombinant Human DKK-3 (Carrier-free)
Cat. No. 21-7102
Alternative Names Dickkopf-related protein-3, Dickkopf-3, dickkopf WNT signaling pathway inhibitor 3, REIC, regulated in Glioma, RIG
Amino Acid Sequence APAPTATSAP VKPGPALSYP QEEATLNEMF REVEELMEDT QHKLRSAVEE MEAEEAAAKA SSEVNLANLP PSYHNETNTD TKVGNNTIHV HREIHKITNN QTGQMVFSET VITSVGDEEG RRSHECIIDE DCGPSMYCQF ASFQYTCQPC RGQRMLCTRD SECCGDQLCV WGHCTKMATR GSNGTICDNQ RDCQPGLCCA FQRGLLFPVC TPLPVEGELC HDPASRLLDL IT
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity An inhibition assay is used to determine activity. The expected ED50 for inhibition of alkaline phosphatase activity in differentiating MC3T3 E1 cells is 2.0-4.0 ng/ml.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human DKK-3 contains 329 amino acid residues and has an expected molecular weight of 36.3 kDa. Due to glycosylation, it migrates at approximately 39-49 kDa by SDS-PAGE analysis under non-reducing conditions.
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source CHO cells
Reactivity Human
Research Areas Bone and Cartilage, Cancer, Neurobiology
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.