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Recombinant Human DKK-2 (Carrier-free)
  • Recombinant Human DKK-2 (Carrier-free)

Recombinant Human DKK-2 (Carrier-free)

Cat No. Size Price Quantity
21-7101-U002 2 µg $69.00
21-7101-U010 10 µg $179.00
21-7101-U100 100 µg $995.00
21-7101-U500 500 µg $2,995.00

Description

The DKK (Dickkopf) family of secreted proteins includes DKK-1 through -4, and a related protein, Soggy (DKK-11). Like DKK-1, DKK-2 can act as an antagonist of the Wnt signaling pathway, forming inhibitory complexes with Wnt co-receptor LRP6 and Kremen-2. However, when LRP6 is overexpressed direct binding of DKK-2 activates canonical Wnt signaling. DKK-2 is expressed in bone, eye and other mesenchymal tissues.
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The DKK (Dickkopf) family of secreted proteins includes DKK-1 through -4, and a related protein, Soggy (DKK-11). Like DKK-1, DKK-2 can act as an antagonist of the Wnt signaling pathway, forming inhibitory complexes with Wnt co-receptor LRP6 and Kremen-2. However, when LRP6 is overexpressed direct binding of DKK-2 activates canonical Wnt signaling. DKK-2 is expressed in bone, eye and other mesenchymal tissues.
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Product Details

Name Recombinant Human DKK-2 (Carrier-free)
Cat. No. 21-7101
Alternative Names Dickkopf-related protein-2, Dickkopf-2, dickkopf WNT signaling pathway inhibitor 2
Amino Acid Sequence SQIGSSRAKL NSIKSSLGGE TPGQAANRSA GMYQGLAFGG SKKGKNLGQA YPCSSDKECE VGRYCHSPHQ GSSACMVCRR KKKRCHRDGM CCPSTRCNNG ICIPVTESIL TPHIPALDGT RHRDRNHGHY SNHDLGWQNL GRPHTKMSHI KGHEGDPCLR SSDCIEGFCC ARHFWTKICK PVLHQGEVCT KQRKKGSHGL EIFQRCDCAK GLSCKVWKDA TYSSKARLHV CQ
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity An inhibition assay is used to determine activity. The expected ED50 for inhibition of alkaline phosphatase activity in differentiating MC3T3 E1 cells is 0.5– 1.0 µg/ml.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human DKK-2 contains 234 amino acid residues and has a calculated molecular weight of ≥ 25.8 kDa. Due to glycosylation, it migrates at approximately 31-36 kDa by SDS-PAGE analysis under non-reducing conditions.
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 98
Source CHO cells
Reactivity Human
Research Areas Bone and Cartilage, Cancer, Neurobiology
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.