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Recombinant Human DKK-1 (Carrier-free)
  • Recombinant Human DKK-1 (Carrier-free)

Recombinant Human DKK-1 (Carrier-free)

Cat No. Size Price Quantity
21-7100-U002 2 µg $69.00
21-7100-U010 10 µg $179.00
21-7100-U100 100 µg $995.00
21-7100-U500 500 µg $2,995.00

Description

The DKK (Dickkopf) family of secreted proteins includes DKK-1 through -4, and a related protein, Soggy (DKK-11). DKK-1 is an antagonist of the Wnt signaling pathway, forming inhibitory complexes with Wnt co-receptors LRP5 or LRP6 along with Kremen-1 or Kremen-2. DKK-1 is expressed during development and, in the adult, is expressed in osteoblasts and osteocytes. Over- or under-expression of DKK-1 can result in decreased or increased bone density.
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The DKK (Dickkopf) family of secreted proteins includes DKK-1 through -4, and a related protein, Soggy (DKK-11). DKK-1 is an antagonist of the Wnt signaling pathway, forming inhibitory complexes with Wnt co-receptors LRP5 or LRP6 along with Kremen-1 or Kremen-2. DKK-1 is expressed during development and, in the adult, is expressed in osteoblasts and osteocytes. Over- or under-expression of DKK-1 can result in decreased or increased bone density.
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Product Details

Name Recombinant Human DKK-1 (Carrier-free)
Cat. No. 21-7100
Alternative Names Dickkopf-related protein-1, Dickkopf-1, SK, SKdickkopf-1 like, dickkopf WNT signaling pathway inhibitor 1
Amino Acid Sequence TLNSVLNSNA IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC GTDEYCASPT RGGDAGVQIC LACRKRRKRC MRHAMCCPGN YCKNGICVSS DQNHFRGEIE ETITESFGND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSRLH TC
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Inhibition of the proliferation of HCT116 colorectal carcinoma cells is used to determine activity. At a DKK-1 concentration of ≥ 200 ng/ml, approximately 40% growth inhibition was achieved.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human DKK-1 has an expected molecular weight of ≥ 25.8 kDa when expressed in HEK293 cells.
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 97
Source HEK293 cells
Reactivity Human
Research Areas Bone and Cartilage, Cancer, Neurobiology
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.