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Recombinant Human CYR61 (Carrier-free)
  • Recombinant Human CYR61 (Carrier-free)

Recombinant Human CYR61 (Carrier-free)

Cat No. Size Price Quantity
21-7175-U005 5 µg $76.00
21-7175-U020 20 µg $175.00
21-7175-U100 100 µg $769.00
21-7175-U500 500 µg $2,295.00

Description

CYR61, also known as CCN1, is a matricellular signaling molecule that binds to a number of cell surface proteins thereby modulating a variety of signaling pathways. CYR61 is expressed in a variety of cells and is capable of regulating cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin αreceptors and heparan sulfate proteoglycans. CYR61 performs critical functions during embryonic development, and in adulthood CYR61 plays important roles in inflammation and tissue repair. It is associated with diseases related to chronic inflammation. Deregulated expression and signaling of CYR61 is associated with a variety of tumors exhibiting both tumorigenic and tumor suppressor properties. When up-regulated it promotes tumorigenesis, angiogenesis, and metastasis in certain cancers and when down-regulated, it suppresses tumor growth in other cancers. Increased expression correlates with the progression and estrogen independence of human breast cancers. Full-length CYR61 protein contains 381 amino acids with an N-terminal secretory signal peptide followed by four structurally distinct domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the Thrombospondin type-I (TSP type-1) domain, and a C-terminal cysteine knot-like domain (CTCK).
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CYR61, also known as CCN1, is a matricellular signaling molecule that binds to a number of cell surface proteins thereby modulating a variety of signaling pathways. CYR61 is expressed in a variety of cells and is capable of regulating cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin αreceptors and heparan sulfate proteoglycans. CYR61 performs critical functions during embryonic development, and in adulthood CYR61 plays important roles in inflammation and tissue repair. It is associated with diseases related to chronic inflammation. Deregulated expression and signaling of CYR61 is associated with a variety of tumors exhibiting both tumorigenic and tumor suppressor properties. When up-regulated it promotes tumorigenesis, angiogenesis, and metastasis in certain cancers and when down-regulated, it suppresses tumor growth in other cancers. Increased expression correlates with the progression and estrogen independence of human breast cancers. Full-length CYR61 protein contains 381 amino acids with an N-terminal secretory signal peptide followed by four structurally distinct domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the Thrombospondin type-I (TSP type-1) domain, and a C-terminal cysteine knot-like domain (CTCK).
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Product Details

Name Recombinant Human CYR61 (Carrier-free)
Cat. No. 21-7175
Alternative Names CCN1, GIGI protein, Cysteine-rich 61
Amino Acid Sequence TCPAACHCPL EAPKCAPGVG LVRDGCGCCK VCAKQLNEDC SKTQPCDHTK GLECNFGASS TALKGICRAQ SEGRPCEYNS RIYQNGESFQ PNCKHQCTCI DGAVGCIPLC PQELSLPNLG CPNPRLVKVT GQCCEEWVCD EDSIKDPMED QDGLLGKELG FDASEVELTR NNELIAVGKG SSLKRLPVFG MEPRILYNPL QGQKCIVQTT SWSQCSKTCG TGISTRVTND NP
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Bioactivity Determined by the dose-dependent stimulation of the proliferation of murine 3T3 cells. The expected ED50 for this effect is 2.0-3.0 μg/ml.
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Molecular Mass Recombinant Human CYR61 is a 39.5 kDa protein containing 357 amino acid residues.
Protein Content Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
Purity (%) 95
Source E.coli
Reactivity Human
Research Areas Angiogenesis & Cardiovascular, Cancer, Proliferation
Reconstitution See Certificate of Analysis (COA) for lot specific reconstitution information.
Application Bioassay
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.