Recombinant Human BDNF (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-8366-U002 | 2 µg | $76.00 | ||
| 21-8366-U010 | 10 µg | $175.00 | ||
| 21-8366-U100 | 100 µg | $990.00 | ||
| 21-8366-U500 | 500 µg | $2,945.00 |
Technical Data Sheet
Request Custom/BulkDescription
Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophic growth factor family of proteins that are critical for the differentiation and survival of several classes of neurons in both the central nervous system and the peripheral nervous system. BDNF binds to and mediates its neurotrophic properties through the tyrosine kinase receptor TrkB. BDNF is synthesized and secreted as a prepropeptide that is proteolytically processed to yield the mature protein. Mouse and Human BDNF sequences are identical.
Recent Citations:
Sutherland TC, Sefiani A, Horvat D, Huntington TE, Lei Y, West AP, Geoffroy CG. Age-Dependent Decline in Neuron Growth Potential and Mitochondria Functions in Cortical Neurons. Cells. 2021 Jun 29;10(7):1625. doi: 10.3390/cells10071625. PMID: 34209640.
Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophic growth factor family of proteins that are critical for the differentiation and survival of several classes of neurons in both the central nervous system and the peripheral nervous system. BDNF binds to and mediates its neurotrophic properties through the tyrosine kinase receptor TrkB. BDNF is synthesized and secreted as a prepropeptide that is proteolytically processed to yield the mature protein. Mouse and Human BDNF sequences are identical.
Recent Citations:
Sutherland TC, Sefiani A, Horvat D, Huntington TE, Lei Y, West AP, Geoffroy CG. Age-Dependent Decline in Neuron Growth Potential and Mitochondria Functions in Cortical Neurons. Cells. 2021 Jun 29;10(7):1625. doi: 10.3390/cells10071625. PMID: 34209640.
HideProduct Details
| Name | Recombinant Human BDNF (Carrier-free) |
|---|---|
| Cat. No. | 21-8366 |
| Alternative Names | Brain Derived Neurotrophic Factor |
| Amino Acid Sequence | MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5-1.0 µg/ml. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant human BDNF is a 27.0 kDa homodimer of two 120 amino acid subunits linked by strong non-covalent interactions. |
| Protein Content | Content Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 98 |
| Source | E. coli |
| Reactivity | Human |
| Research Areas | Neurobiology; Proliferation; Stem Cells & Differentiation |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.