Recombinant Human Angiopoietin-2 (Carrier-free)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 21-7168-U005 | 5 µg | $76.00 | ||
| 21-7168-U020 | 20 µg | $175.00 | ||
| 21-7168-U100 | 100 µg | $799.00 | ||
| 21-7168-U250 | 250 µg | $1,725.00 | ||
| 21-7168-U500 | 500 µg | $2,995.00 |
Lead time: 2-3 business days
Technical Data Sheet
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Angiopoietin-2 (ANG-2, ANGPT2) is a secreted glycoprotein belonging to the angiopoietin family of growth factors that play a critical role in the regulation of angiogenesis and vascular development. Like Angiopoietin-1, Angiopoietin-2 is a ligand for receptor tyrosine kinase Tie-2, binding with similar affinity. Angiopoietin-2 plays a role in regulating Ang-1/Tie-2 signaling, but can also act as a Tie-2 antagonist depending on its state of multimerization. In the absence of VEGF, Angiopoietin-2 promotes vessel destabilization and regression. On the contrary, in the presence of VEGF, it promotes cell proliferation and migration in endothelial cells.
Angiopoietin-2 (ANG-2, ANGPT2) is a secreted glycoprotein belonging to the angiopoietin family of growth factors that play a critical role in the regulation of angiogenesis and vascular development. Like Angiopoietin-1, Angiopoietin-2 is a ligand for receptor tyrosine kinase Tie-2, binding with similar affinity. Angiopoietin-2 plays a role in regulating Ang-1/Tie-2 signaling, but can also act as a Tie-2 antagonist depending on its state of multimerization. In the absence of VEGF, Angiopoietin-2 promotes vessel destabilization and regression. On the contrary, in the presence of VEGF, it promotes cell proliferation and migration in endothelial cells.
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Product Details
| Name | Recombinant Human Angiopoietin-2 (Carrier-free) |
|---|---|
| Cat. No. | 21-7168 |
| Alternative Names | ANGPT2, ANG-2 |
| Amino Acid Sequence | DAPLEYDDSV QRLQVLENIM ENNTQWLMKL ENYIQDNMKK EMVEIQQNAV QNQTAVMIEI GTNLLNQTAE QTRKLTDVEA QVLNQTTRLE LQLLEHSLST NKLEKQILDQ TSEINKLQDK NSFLEKKVLA MEDKHIIQLQ SIKEEKDQLQ VLVSKQNSII EELEKKIVTA TVNNSVLQKQ QHDLMETVNN LLTMMSTSNS AKDPTVAKEE QISFRDCAEV FKSGHTTNGI YT |
| Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
| Bioactivity | Using a concentration of 0.2 ug/mL, a tubulogenesis assay with human umbilical vein endothelial cells (HUVECs) is performed. |
| Endotoxin Level | Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg). |
| Molecular Mass | Recombinant Human Angiopoietin-2 is a 50.1 kDa glycoprotein with a C-terminal His-tag. It migrates under reducing conditions at approximately 60-70 kDa by SDS-PAGE. Based on sequencing, the N-terminus starts with residue 68 (D) of the precursor protein. |
| Protein Content | Verified by UV Spectroscopy and/or SDS-PAGE gel. |
| Purity (%) | 95 |
| Source | CHO cells |
| Reactivity | Human |
| Research Areas | Apoptosis, Angiogensis & Cardiovascular, Bone and Cartidage |
| Reconstitution | See Certificate of Analysis (COA) for lot specific reconstitution information. |
| Application | Bioassay |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.