RBC Lysis Buffer (10X)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| TNB-4300-L100 | 100 mL | $36.00 |
Technical Data Sheet
Request Custom/BulkDescription
RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent. The diluted 1X working solution will lyse red blood cells in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer (10X) does not contain a fixative so the cells remain viable after red blood cell lysis.
Download the Lysing Protocol.
Recent Publications:
Xia L, Shi Y, Su J, Friedemann T, et al. Shufeng Jiedu, a promising herbal therapy for moderate COVID-19:Antiviral and anti-inflammatory properties, pathways of bioactive compounds, and a clinical real-world pragmatic study. Phytomedicine. 2020 Oct 22:153390. doi: 10.1016/j.phymed.2020.153390. PMID: 33158717; PMCID: PMC7581328.
Jennifer S. Chen, Jessica D.S. Grassmann, Uthaman Gowthaman, Sam J. Olyha, Tregony Simoneau, M. Cecilia Berin, Stephanie C. Eisenbarth, Adam Williams. Flow cytometric identification of Tfh13 cells in mouse and human. Journal of Allergy and Clinical Immunology, 2020. https://doi.org/10.1016/j.jaci.2020.04.063.
Jackson LP, Tjoa BA, Mellert H, Pestano GA. Development of a TCR beta repertoire assay for profiling liquid biopsies from NSCLC donors. Cancer Drug Resist 2020;3. http://dx.doi.org/10.20517/cdr.2020.07.
Kim IS, Gao Y, Welte T, et al. Immuno-subtyping of breast cancer reveals distinct myeloid cell profiles and immunotherapy resistance mechanisms. Nat Cell Biol. 2019;21(9):1113-1126. doi:10.1038/s41556-019-0373-7.
RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent. The diluted 1X working solution will lyse red blood cells in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer (10X) does not contain a fixative so the cells remain viable after red blood cell lysis.
Download the Lysing Protocol.
Recent Publications:
Xia L, Shi Y, Su J, Friedemann T, et al. Shufeng Jiedu, a promising herbal therapy for moderate COVID-19:Antiviral and anti-inflammatory properties, pathways of bioactive compounds, and a clinical real-world pragmatic study. Phytomedicine. 2020 Oct 22:153390. doi: 10.1016/j.phymed.2020.153390. PMID: 33158717; PMCID: PMC7581328.
Jennifer S. Chen, Jessica D.S. Grassmann, Uthaman Gowthaman, Sam J. Olyha, Tregony Simoneau, M. Cecilia Berin, Stephanie C. Eisenbarth, Adam Williams. Flow cytometric identification of Tfh13 cells in mouse and human. Journal of Allergy and Clinical Immunology, 2020. https://doi.org/10.1016/j.jaci.2020.04.063.
Jackson LP, Tjoa BA, Mellert H, Pestano GA. Development of a TCR beta repertoire assay for profiling liquid biopsies from NSCLC donors. Cancer Drug Resist 2020;3. http://dx.doi.org/10.20517/cdr.2020.07.
Kim IS, Gao Y, Welte T, et al. Immuno-subtyping of breast cancer reveals distinct myeloid cell profiles and immunotherapy resistance mechanisms. Nat Cell Biol. 2019;21(9):1113-1126. doi:10.1038/s41556-019-0373-7.
Product Details
| Name | RBC Lysis Buffer (10X) |
|---|---|
| Cat. No. | TNB-4300 |
| Protocol | RBC Lysing Protocol |
| Application |
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.