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Human peripheral blood cells were lysed with RBC Lysis Buffer (TNB-4300-L100). The scatter profile of lysed cells is shown.
  • Human peripheral blood cells were lysed with RBC Lysis Buffer (TNB-4300-L100). The scatter profile of lysed cells is shown.

RBC Lysis Buffer (10X)

Cat No. Size Price Quantity
TNB-4300-L100 100 mL $36.00

Description

RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent. The diluted 1X working solution will lyse red blood cells in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer (10X) does not contain a fixative so the cells remain viable after red blood cell lysis.

 

Lysing ProtocolDownload the Lysing Protocol.

Recent Publications:

Xia L, Shi Y, Su J, Friedemann T, et al. Shufeng Jiedu, a promising herbal therapy for moderate COVID-19:Antiviral and anti-inflammatory properties, pathways of bioactive compounds, and a clinical real-world pragmatic study. Phytomedicine. 2020 Oct 22:153390. doi: 10.1016/j.phymed.2020.153390. PMID: 33158717; PMCID: PMC7581328. 

» View on ScienceDirect

Jennifer S. Chen, Jessica D.S. Grassmann, Uthaman Gowthaman, Sam J. Olyha, Tregony Simoneau, M. Cecilia Berin, Stephanie C. Eisenbarth, Adam Williams. Flow cytometric identification of Tfh13 cells in mouse and human. Journal of Allergy and Clinical Immunology, 2020.  https://doi.org/10.1016/j.jaci.2020.04.063.

» View on ScienceDirect

Jackson LP, Tjoa BA, Mellert H, Pestano GA. Development of a TCR beta repertoire assay for profiling liquid biopsies from NSCLC donors. Cancer Drug Resist 2020;3. http://dx.doi.org/10.20517/cdr.2020.07.

» View on Cancer Drug Resist

Kim IS, Gao Y, Welte T, et al. Immuno-subtyping of breast cancer reveals distinct myeloid cell profiles and immunotherapy resistance mechanisms. Nat Cell Biol. 2019;21(9):1113-1126. doi:10.1038/s41556-019-0373-7.

» View on PubMed

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RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent. The diluted 1X working solution will lyse red blood cells in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer (10X) does not contain a fixative so the cells remain viable after red blood cell lysis.

 

Lysing ProtocolDownload the Lysing Protocol.

Recent Publications:

Xia L, Shi Y, Su J, Friedemann T, et al. Shufeng Jiedu, a promising herbal therapy for moderate COVID-19:Antiviral and anti-inflammatory properties, pathways of bioactive compounds, and a clinical real-world pragmatic study. Phytomedicine. 2020 Oct 22:153390. doi: 10.1016/j.phymed.2020.153390. PMID: 33158717; PMCID: PMC7581328. 

» View on ScienceDirect

Jennifer S. Chen, Jessica D.S. Grassmann, Uthaman Gowthaman, Sam J. Olyha, Tregony Simoneau, M. Cecilia Berin, Stephanie C. Eisenbarth, Adam Williams. Flow cytometric identification of Tfh13 cells in mouse and human. Journal of Allergy and Clinical Immunology, 2020.  https://doi.org/10.1016/j.jaci.2020.04.063.

» View on ScienceDirect

Jackson LP, Tjoa BA, Mellert H, Pestano GA. Development of a TCR beta repertoire assay for profiling liquid biopsies from NSCLC donors. Cancer Drug Resist 2020;3. http://dx.doi.org/10.20517/cdr.2020.07.

» View on Cancer Drug Resist

Kim IS, Gao Y, Welte T, et al. Immuno-subtyping of breast cancer reveals distinct myeloid cell profiles and immunotherapy resistance mechanisms. Nat Cell Biol. 2019;21(9):1113-1126. doi:10.1038/s41556-019-0373-7.

» View on PubMed

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Product Details

Name RBC Lysis Buffer (10X)
Cat. No. TNB-4300
Protocol RBC Lysing Protocol
Application
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.