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Purified Anti-Mouse CD16 / CD32 (Fc Shield) (2.4G2)
  • Purified Anti-Mouse CD16 / CD32 (Fc Shield) (2.4G2)

Purified Anti-Mouse CD16 / CD32 (Fc Shield) (2.4G2)

Cat No. Size Price Quantity
70-0161-U100 100 µg $53.00
70-0161-U500 500 µg $115.00
70-0161-M001 1 mg $230.00

Description

The 2.4G2 antibody is specific for a common epitope found in the extracellular regions of mouse Fc-receptors Fc-gamma II (CD32) and Fc-gamma III (CD16). As these are receptors for the Fc portion of mouse IgG, they may also bind laboratory antibody preparations and products used in a variety of cell analysis protocols such as flow cytometry, immunohistochemistry and functional cell assays. The 2.4G2 antibody is therefore widely used as a pre-treatment reagent to block binding of specific antibodies of interest, e.g. fluorescently conjugated antibodies, to Fc receptors via their Fc domains and contributing to “non-specific” staining.

Recent Publications:

Shen M, Song Y, Ichinose T, et al. In vivo immune activation of splenocytes following exposure to tar from Asian sand dust. J Toxicol Environ Health A. 2020;1-10. doi:10.1080/15287394.2020.1806160.  

» View on PubMed

Yamamuro T, Kawabata T, Fukuhara A, et al. Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy. Nat Commun. 2020;11(1):4150. Published 2020 Aug 18. doi:10.1038/s41467-020-17985-w.  

 » View on Nature Communications

Wu B, Chiang HC, Sun X, et al. Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation. J Immunother Cancer. 2020;8(2):e000964. doi:10.1136/jitc-2020-000964.

» View on PubMed

Kajihara N, Kitagawa F, Kobayashi T, Wada H, Otsuka R, Seino KI. Interleukin-34 contributes to poor prognosis in triple-negative breast cancer [published online ahead of print, 2020 Jun 23]. Breast Cancer. 2020;10.1007/s12282-020-01123-x. doi:10.1007/s12282-020-01123-x.

» View on PubMed

Wormsbaecher C, Hindman AR, Avendano A, et al. In utero estrogenic endocrine disruption alters the stroma to increase extracellular matrix density and mammary gland stiffness. Breast Cancer Res. 2020;22(1):41. Published 2020 May 5. doi:10.1186/s13058-020-01275-w.

» View on PubMed

McDaniel MM, Kottyan LC, Singh H, Pasare C. Suppression of Inflammasome Activation by IRF8 and IRF4 in cDCs Is Critical for T Cell Priming. Cell Rep. 2020 May 5;31(5):107604. doi: 10.1016/j.celrep.2020.107604

» View on PubMed

Markman, J.L., Porritt, R.A., Wakita, D. et al. Loss of testosterone impairs anti-tumor neutrophil function. Nat Commun 11, 1613 (2020). https://doi.org/10.1038/s41467-020-15397-4.

» View on Nature Communications

Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059

» View on PubMed

Kim IS, Gao Y, Welte T, et al. Immuno-subtyping of breast cancer reveals distinct myeloid cell profiles and immunotherapy resistance mechanisms. Nat Cell Biol. 2019;21(9):1113-1126. doi:10.1038/s41556-019-0373-7.

» View on PubMed

Sarika Saraswati, Stephanie M.W. Marrow, Lester A. Watch1 & Pampee P. Young. Identification of a pro-angiogenic functional role for FSP1-positive fibroblast subtype in wound healing. Nat Commun. 2019 Jul 9;10(1):3027. doi: 10.1038/s41467-019-10965-9

» View on PubMed

Yang BH, Wang K, Wan S, et al. TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases. Cell Rep. 2019;27(12):3629-3645.e6. doi:10.1016/j.celrep.2019.05.061.

» View on PubMed

Dutta R, Lunzer MM, Auger JL, Akgün E, Portoghese PS, Binstadt BA. A bivalent compound targeting CCR5 and the mu opioid receptor treats inflammatory arthritis pain in mice without inducing pharmacologic tolerance. Arthritis Res Ther. 2018;20(1):154. Published 2018 Jul 27. doi:10.1186/s13075-018-1661-5.

 » View on PubMed

Guo M, Price MJ, Patterson DG, et al. EZH2 Represses the B Cell Transcriptional Program and Regulates Antibody-Secreting Cell Metabolism and Antibody Production. J Immunol. 2018;200(3):1039-1052. doi:10.4049/jimmunol.1701470.

» View on PubMed

Adami AJ, Bracken SJ, Guernsey LA, et al. Early-life antibiotics attenuate regulatory T cell generation and increase the severity of murine house dust mite-induced asthma. Pediatr Res. 2018;84(3):426-434. doi:10.1038/s41390-018-0031-y.

» View on PubMed

Grinberg-Bleyer Y, Oh H, Desrichard A, Bhatt DM, Caron R, Chan TA, Schmid RM, Klein U, Hayden MS and Ghosh S. 2017. NF-κB c-Rel Is Crucial for the Regulatory T Cell Immune Checkpoint in Cancer. Cell. doi: 10.1016/j.cell.2017.08.004. 

» View on PubMed

Benck CJ, Martinov T, Fife BT and Chatterjea D. 2016. J. Vis. Exp. (107), e53638, doi:10.3791/53638. (Blocking - Flow Cytometry)

 

Shim YR and Lee HK. 2015. Immune Netw. 15(2): 73-82. (Blocking - Flow Cytometry)

 

Ishizuka M, Miyazaki Y, Masuo M, Shuara K, Tateishi T, Yasui M and Inase N. 2015. PLoS One. doi: 10.1371/journal.pone.0137978. (Blocking - Flow Cytometry)

 

Oh JE, Lee MS, Kim YJ and Lee HK. 2016. Sci Rep. doi: 10.1038/srep19089. (Blocking - Flow Cytometry)
View More

The 2.4G2 antibody is specific for a common epitope found in the extracellular regions of mouse Fc-receptors Fc-gamma II (CD32) and Fc-gamma III (CD16). As these are receptors for the Fc portion of mouse IgG, they may also bind laboratory antibody preparations and products used in a variety of cell analysis protocols such as flow cytometry, immunohistochemistry and functional cell assays. The 2.4G2 antibody is therefore widely used as a pre-treatment reagent to block binding of specific antibodies of interest, e.g. fluorescently conjugated antibodies, to Fc receptors via their Fc domains and contributing to “non-specific” staining.

Recent Publications:

Shen M, Song Y, Ichinose T, et al. In vivo immune activation of splenocytes following exposure to tar from Asian sand dust. J Toxicol Environ Health A. 2020;1-10. doi:10.1080/15287394.2020.1806160.  

» View on PubMed

Yamamuro T, Kawabata T, Fukuhara A, et al. Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy. Nat Commun. 2020;11(1):4150. Published 2020 Aug 18. doi:10.1038/s41467-020-17985-w.  

 » View on Nature Communications

Wu B, Chiang HC, Sun X, et al. Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation. J Immunother Cancer. 2020;8(2):e000964. doi:10.1136/jitc-2020-000964.

» View on PubMed

Kajihara N, Kitagawa F, Kobayashi T, Wada H, Otsuka R, Seino KI. Interleukin-34 contributes to poor prognosis in triple-negative breast cancer [published online ahead of print, 2020 Jun 23]. Breast Cancer. 2020;10.1007/s12282-020-01123-x. doi:10.1007/s12282-020-01123-x.

» View on PubMed

Wormsbaecher C, Hindman AR, Avendano A, et al. In utero estrogenic endocrine disruption alters the stroma to increase extracellular matrix density and mammary gland stiffness. Breast Cancer Res. 2020;22(1):41. Published 2020 May 5. doi:10.1186/s13058-020-01275-w.

» View on PubMed

McDaniel MM, Kottyan LC, Singh H, Pasare C. Suppression of Inflammasome Activation by IRF8 and IRF4 in cDCs Is Critical for T Cell Priming. Cell Rep. 2020 May 5;31(5):107604. doi: 10.1016/j.celrep.2020.107604

» View on PubMed

Markman, J.L., Porritt, R.A., Wakita, D. et al. Loss of testosterone impairs anti-tumor neutrophil function. Nat Commun 11, 1613 (2020). https://doi.org/10.1038/s41467-020-15397-4.

» View on Nature Communications

Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059

» View on PubMed

Kim IS, Gao Y, Welte T, et al. Immuno-subtyping of breast cancer reveals distinct myeloid cell profiles and immunotherapy resistance mechanisms. Nat Cell Biol. 2019;21(9):1113-1126. doi:10.1038/s41556-019-0373-7.

» View on PubMed

Sarika Saraswati, Stephanie M.W. Marrow, Lester A. Watch1 & Pampee P. Young. Identification of a pro-angiogenic functional role for FSP1-positive fibroblast subtype in wound healing. Nat Commun. 2019 Jul 9;10(1):3027. doi: 10.1038/s41467-019-10965-9

» View on PubMed

Yang BH, Wang K, Wan S, et al. TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases. Cell Rep. 2019;27(12):3629-3645.e6. doi:10.1016/j.celrep.2019.05.061.

» View on PubMed

Dutta R, Lunzer MM, Auger JL, Akgün E, Portoghese PS, Binstadt BA. A bivalent compound targeting CCR5 and the mu opioid receptor treats inflammatory arthritis pain in mice without inducing pharmacologic tolerance. Arthritis Res Ther. 2018;20(1):154. Published 2018 Jul 27. doi:10.1186/s13075-018-1661-5.

 » View on PubMed

Guo M, Price MJ, Patterson DG, et al. EZH2 Represses the B Cell Transcriptional Program and Regulates Antibody-Secreting Cell Metabolism and Antibody Production. J Immunol. 2018;200(3):1039-1052. doi:10.4049/jimmunol.1701470.

» View on PubMed

Adami AJ, Bracken SJ, Guernsey LA, et al. Early-life antibiotics attenuate regulatory T cell generation and increase the severity of murine house dust mite-induced asthma. Pediatr Res. 2018;84(3):426-434. doi:10.1038/s41390-018-0031-y.

» View on PubMed

Grinberg-Bleyer Y, Oh H, Desrichard A, Bhatt DM, Caron R, Chan TA, Schmid RM, Klein U, Hayden MS and Ghosh S. 2017. NF-κB c-Rel Is Crucial for the Regulatory T Cell Immune Checkpoint in Cancer. Cell. doi: 10.1016/j.cell.2017.08.004. 

» View on PubMed

Benck CJ, Martinov T, Fife BT and Chatterjea D. 2016. J. Vis. Exp. (107), e53638, doi:10.3791/53638. (Blocking - Flow Cytometry)

 

Shim YR and Lee HK. 2015. Immune Netw. 15(2): 73-82. (Blocking - Flow Cytometry)

 

Ishizuka M, Miyazaki Y, Masuo M, Shuara K, Tateishi T, Yasui M and Inase N. 2015. PLoS One. doi: 10.1371/journal.pone.0137978. (Blocking - Flow Cytometry)

 

Oh JE, Lee MS, Kim YJ and Lee HK. 2016. Sci Rep. doi: 10.1038/srep19089. (Blocking - Flow Cytometry)
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Product Details

Name Purified Anti-Mouse CD16 / CD32 (Fc Shield) (2.4G2)
Cat. No. 70-0161
Alternative Names FCGR3, IGFR3; FCGR2, IGFR2; FC Receptor
Gene ID 14130/14131
Clone 2.4G2
Isotype Rat IgG2b
Reactivity Mouse
Cross Reactivity
Format Purified
Application Flow Cytometry, IF, IP
Citations*

Shen M, Song Y, Ichinose T, et al. In vivo immune activation of splenocytes following exposure to tar from Asian sand dust. J Toxicol Environ Health A. 2020;1-10. doi:10.1080/15287394.2020.1806160.  

» View on PubMed

Yamamuro T, Kawabata T, Fukuhara A, et al. Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy. Nat Commun. 2020;11(1):4150. Published 2020 Aug 18. doi:10.1038/s41467-020-17985-w.  

 » View on Nature Communications

Wu B, Chiang HC, Sun X, et al. Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation. J Immunother Cancer. 2020;8(2):e000964. doi:10.1136/jitc-2020-000964.

» View on PubMed

Kajihara N, Kitagawa F, Kobayashi T, Wada H, Otsuka R, Seino KI. Interleukin-34 contributes to poor prognosis in triple-negative breast cancer [published online ahead of print, 2020 Jun 23]. Breast Cancer. 2020;10.1007/s12282-020-01123-x. doi:10.1007/s12282-020-01123-x.

» View on PubMed

McDaniel MM, Kottyan LC, Singh H, Pasare C. Suppression of Inflammasome Activation by IRF8 and IRF4 in cDCs Is Critical for T Cell Priming. Cell Rep. 2020 May 5;31(5):107604. doi: 10.1016/j.celrep.2020.107604

» View on PubMed

Grinberg-Bleyer Y, Oh H, Desrichard A, Bhatt DM, Caron R, Chan TA, Schmid RM, Klein U, Hayden MS and Ghosh S. 2017. NF-κB c-Rel Is Crucial for the Regulatory T Cell Immune Checkpoint in Cancer. Cell. doi: 10.1016/j.cell.2017.08.004. 

» View on PubMed

Sarika Saraswati, Stephanie M.W. Marrow, Lester A. Watch1 & Pampee P. Young. Identification of a pro-angiogenic functional role for FSP1-positive fibroblast subtype in wound healing. Nat Commun. 2019 Jul 9;10(1):3027. doi: 10.1038/s41467-019-10965-9

» View on PubMed

Yang BH, Wang K, Wan S, et al. TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases. Cell Rep. 2019;27(12):3629-3645.e6. doi:10.1016/j.celrep.2019.05.061.

» View on PubMed

Guo M, Price MJ, Patterson DG, et al. EZH2 Represses the B Cell Transcriptional Program and Regulates Antibody-Secreting Cell Metabolism and Antibody Production. J Immunol. 2018;200(3):1039-1052. doi:10.4049/jimmunol.1701470.

» View on PubMed

Adami AJ, Bracken SJ, Guernsey LA, et al. Early-life antibiotics attenuate regulatory T cell generation and increase the severity of murine house dust mite-induced asthma. Pediatr Res. 2018;84(3):426-434. doi:10.1038/s41390-018-0031-y.

» View on PubMed

Stephen TL, Wilson BS, and Laufer TM. 2012. Proc. Natl. Acad. Sci. 109: 7415-7420. (Blocking- Immunofluorescence microscopy)

Yamaji O, Nagaishi T, Totsuka T, Onizawa M, Suzuki M, Tsuge et al. 2012. J. Immunol. 188:2524-2536. (Blocking - in vitro)

Shimazu T, Iida R, Zhang Q, Welner RS, Medina KL, Alberola-Ila J, and Kincade PW. 2012. Blood. 119:4889-4897. (Blocking – Flow cytometry)

Stoeker L, Nordone S, Gunderson S, Zhang L, Kajikawa A, LaVoy A, Miller M, Klaenhammer TR, and Dean GA. 2011. Clin. Vaccine Immunol. 18: 1834-1844. (Blocking – Flow cytometry)

Beretta F, St.-Pierre J, Piccirillo CA, and Stevenson MM. 2011. J. Immunol. 186: 4862-4871. (Blocking - Flow cytometry)

Coudert JD, Scarpellino L, Gros F, Vivier E, and Held W. 2008 Blood. 111: 3571-3578. (Immunoprecipitation)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.