Propidium Iodide
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 13-6990-T200 | 200 tests | $29.00 | ||
| 13-6990-T500 | 500 tests | $57.00 |
Technical Data Sheet
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Propidium Iodide (PI) is a membrane-impermeant DNA binding dye that cannot penetrate viable cells. PI rapidly enters cells with compromised membranes and intercalates between ≥ ≥ ≥ base pairs allowing exclusion of non-viable cells from analysis of flow cytometry data. PI has a broad emission spectrum from 535-617 nm and can be detected in either FL2 or FL3 detectors. When used with Annexin V FITC, it is recommended to analyze PI in FL2.
Propidium Iodide (PI) is a membrane-impermeant DNA binding dye that cannot penetrate viable cells. PI rapidly enters cells with compromised membranes and intercalates between ≥ ≥ ≥ base pairs allowing exclusion of non-viable cells from analysis of flow cytometry data. PI has a broad emission spectrum from 535-617 nm and can be detected in either FL2 or FL3 detectors. When used with Annexin V FITC, it is recommended to analyze PI in FL2.
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Product Details
| Name | Propidium Iodide |
|---|---|
| Cat. No. | 13-6990 |
| Protocol | Propidium Iodide or 7-AAD Viability Staining Protocol |
| Excitation Laser | Blue (488 nm), Green (532 nm), Yellow-Green (561 nm) |
| Emission (nm) | 535 - 617 |
| Formulation | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2 |
| Application | Flow Cytometry |
Application Key:
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.