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C57Bl/6 splenocytes were stained with 0.125 ug PerCP-Cy5.5 Anti-Mouse TCR beta (65-5961) (solid line) or 0.125 ug PerCP-Cy5.5 Armenian hamster IgG isotype control (dashed line).
  • C57Bl/6 splenocytes were stained with 0.125 ug PerCP-Cy5.5 Anti-Mouse TCR beta (65-5961) (solid line) or 0.125 ug PerCP-Cy5.5 Armenian hamster IgG isotype control (dashed line).
  • PerCP-Cyanine5.5 Anti-Mouse TCR beta (H57-597)

PerCP-Cyanine5.5 Anti-Mouse TCR beta (H57-597)

Cat No. Size Price Quantity
65-5961-U025 25 µg $60.00
65-5961-U100 100 µg $154.00

Description

The H57-597 antibody is specific for the beta chain of the mouse T cell Receptor (TCR). This cell surface protein combines with a second protein chain (alpha chain) to form the alpha-beta TCR that is expressed by NK1.1+ thymocytes, NKT cells, and the majority of peripheral T cells. A small number of T cells may express an alternative heteromer of gamma/delta protein chains, known as the g/d TCR. These receptors participate in a complex with CD3, and with the co-receptors CD4 or CD8, to recognize and respond to antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death.

The H57-597 antibody is used as a phenotypic marker for T cells expressing the alpha-beta TCR. It is also widely used to cross-link surface TCR and thereby mimic TCR-mediated cell activation or induction of apoptosis. The antibody does not cross-react with cells expressing the g/d TCR.

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The H57-597 antibody is specific for the beta chain of the mouse T cell Receptor (TCR). This cell surface protein combines with a second protein chain (alpha chain) to form the alpha-beta TCR that is expressed by NK1.1+ thymocytes, NKT cells, and the majority of peripheral T cells. A small number of T cells may express an alternative heteromer of gamma/delta protein chains, known as the g/d TCR. These receptors participate in a complex with CD3, and with the co-receptors CD4 or CD8, to recognize and respond to antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death.

The H57-597 antibody is used as a phenotypic marker for T cells expressing the alpha-beta TCR. It is also widely used to cross-link surface TCR and thereby mimic TCR-mediated cell activation or induction of apoptosis. The antibody does not cross-react with cells expressing the g/d TCR.

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Product Details

Name PerCP-Cyanine5.5 Anti-Mouse TCR beta (H57-597)
Cat. No. 65-5961
Alternative Names TCRb, TCRbeta, TCR-b chain, TCR-b, b-TCR
Gene ID 21577
Clone H57-597
Isotype Armenian Hamster IgG
Reactivity Mouse
Cross Reactivity
Format PerCP-Cyanine5.5
Application Flow Cytometry
Citations*

Qiumei Du, Ashley R. Hoover, Igor Dozmorov, Ondine B. Cleaver,Joshua T. Mendell, Nicolai S.C. van Oers. MIR205HG Is a Long Noncoding RNA that Regulates Growth Hormone and Prolactin Production in the Anterior Pituitary. Dev Cell. 2019 May 20;49(4):618-631.e5. doi: 10.1016/j.devcel.2019.03.012

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Avery L, Filderman J, Szymczak-Workman AL, Kane LP4. Tim-3 co-stimulation promotes short-lived effector T cells, restricts memory precursors, and is dispensable for T cell exhaustion. Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2455-2460. doi: 10.1073/pnas.1712107115

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Grinberg-Bleyer Y, Oh H, Desrichard A, Bhatt DM, Caron R, Chan TA, Schmid RM, Klein U, Hayden MS and Ghosh S. 2017. NF-κB c-Rel Is Crucial for the Regulatory T Cell Immune Checkpoint in Cancer. Cell. doi: 10.1016/j.cell.2017.08.004. 

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Berent-Maoz B, Montecino-Rodriguez E, Signer RAJ, and Dorshkind K. 2012. Blood. 199:5715-5721. (Flow cytometry)

Wang D, Qin H, Du W, Shen Y-W, Lee W-H, Riggs AD, and Liu C-P. 2012. Proc. Natl. Acad. Sci. 109:9493-9498. (in vitro induction of apoptosis)

O’Brian RL, Taylor MA, Hartley J, Nuhsbaum T, Dugan S, Lahmers K, Aydintug MK, Wands JM, Roark CL, and Born WK. 2009. Invest. Ophthalmol. Vis. Sci. 50: 3266-3274. (Immunofluorescence microscopy – OCT embedded frozen tissue)

Matei IR, Gladdy RA, Nutter LMJ, Canty A, Guidos CJ, and Danska JS. 2007. Blood. 109:1887-1896. (Immunoprecipitation)

Harada N, Shimada M, Okano S, Suehiro T, Soejima Y, Tomita Y, and Maehara Y. 2004. J. Immunol. 173:6635-6644. (in vivo T cell depletion)

Kubo RT, Born W, Kappler JW, Marrack P, and Pigeon M. 1989. J. Immunol. 142: 2736-2742. (Origination of clone, Immunoprecipitation, in vitro activation)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.