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C57Bl/6 bone marrow cells were stained with 0.25 ug PerCP-Cy5.5 Anti-Hu/Mo CD11b (65-0112) (solid line) or 0.25 ug PerCP-Cy5.5 Rat IgG2b isotype control (dashed line).
  • C57Bl/6 bone marrow cells were stained with 0.25 ug PerCP-Cy5.5 Anti-Hu/Mo CD11b (65-0112) (solid line) or 0.25 ug PerCP-Cy5.5 Rat IgG2b isotype control (dashed line).
  • PerCP-Cyanine5.5 Anti-Human/Mouse CD11b (M1/70)

PerCP-Cyanine5.5 Anti-Human/Mouse CD11b (M1/70)

Cat No. Size Price Quantity
65-0112-U025 25 µg $48.00
65-0112-U100 100 µg $154.00

Description

The M1/70 antibody reacts with human and mouse CD11b, also known as integrin αalpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin αreceptors that mediate adhesion between ≥ ≥ ≥ cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrin αs are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin αreceptors consist of distinct combinations of integrin αchains which are differentially expressed. integrin αalpha M (CD11b) assembles with integrin αbeta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.

The M1/70 antibody is widely used as a marker for CD11b expression on mouse macrophages, granulocytes, neutrophils, and NK cells. The antibody is also reported to be cross-reactive for Rhesus macaque CD11b.

Recent Publications:

Hiroshi Kobayashi and Keiyo Takubo. Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells. STAR Protocols.  Published 2 August 2020. https://doi.org/10.1016/j.xpro.2020.100078. 

» View on ScienceDirect

Andersen TK, Huszthy PC, Gopalakrishnan RP, et al. Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules. NPJ Vaccines. 2019;4:9. Published 2019 Feb 11. doi:10.1038/s41541-019-0101-0.

» View on PubMed

Kobayashi H, Morikawa T, Okinaga A, et al. Environmental Optimization Enables Maintenance of Quiescent Hematopoietic Stem Cells Ex Vivo. Cell Rep. 2019;28(1):145-158.e9. doi:10.1016/j.celrep.2019.06.008.

» View on PubMed

Udden SN, Kwak YT, Godfrey V, et al. NLRP12 suppresses hepatocellular carcinoma via downregulation of cJun N-terminal kinase activation in the hepatocyte. Elife. 2019;8:e40396. Published 2019 Apr 16. doi:10.7554/eLife.40396.

» View on PubMed

Abe Y, Kamachi F, Kawamoto T, Makino F, Ito J, Kojima Y, Moustapha AEDH, Usui Y, Yagita H, Takasaki Y, Okumura K and Akiba H. 2013. J. Immunol. 191:4562-4572. (Flow Cytometry)

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The M1/70 antibody reacts with human and mouse CD11b, also known as integrin αalpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin αreceptors that mediate adhesion between ≥ ≥ ≥ cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrin αs are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin αreceptors consist of distinct combinations of integrin αchains which are differentially expressed. integrin αalpha M (CD11b) assembles with integrin αbeta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.

The M1/70 antibody is widely used as a marker for CD11b expression on mouse macrophages, granulocytes, neutrophils, and NK cells. The antibody is also reported to be cross-reactive for Rhesus macaque CD11b.

Recent Publications:

Hiroshi Kobayashi and Keiyo Takubo. Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells. STAR Protocols.  Published 2 August 2020. https://doi.org/10.1016/j.xpro.2020.100078. 

» View on ScienceDirect

Andersen TK, Huszthy PC, Gopalakrishnan RP, et al. Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules. NPJ Vaccines. 2019;4:9. Published 2019 Feb 11. doi:10.1038/s41541-019-0101-0.

» View on PubMed

Kobayashi H, Morikawa T, Okinaga A, et al. Environmental Optimization Enables Maintenance of Quiescent Hematopoietic Stem Cells Ex Vivo. Cell Rep. 2019;28(1):145-158.e9. doi:10.1016/j.celrep.2019.06.008.

» View on PubMed

Udden SN, Kwak YT, Godfrey V, et al. NLRP12 suppresses hepatocellular carcinoma via downregulation of cJun N-terminal kinase activation in the hepatocyte. Elife. 2019;8:e40396. Published 2019 Apr 16. doi:10.7554/eLife.40396.

» View on PubMed

Abe Y, Kamachi F, Kawamoto T, Makino F, Ito J, Kojima Y, Moustapha AEDH, Usui Y, Yagita H, Takasaki Y, Okumura K and Akiba H. 2013. J. Immunol. 191:4562-4572. (Flow Cytometry)

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Product Details

Name PerCP-Cyanine5.5 Anti-Human/Mouse CD11b (M1/70)
Cat. No. 65-0112
Alternative Names Mac-1, integrin α?M, CR3, ITGAM
Gene ID 16409 / 3684
Clone M1/70
Isotype Rat IgG2b, κ
Reactivity Human, Mouse
Cross Reactivity Chimpanzee, Baboon, Cynomolgus, Rhesus
Format PerCP-Cyanine5.5
Application Flow Cytometry
Citations*

Hiroshi Kobayashi and Keiyo Takubo. Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells. STAR Protocols.  Published 2 August 2020. https://doi.org/10.1016/j.xpro.2020.100078. 

» View on ScienceDirect

Andersen TK, Huszthy PC, Gopalakrishnan RP, et al. Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules. NPJ Vaccines. 2019;4:9. Published 2019 Feb 11. doi:10.1038/s41541-019-0101-0.

» View on PubMed

Kobayashi H, Morikawa T, Okinaga A, et al. Environmental Optimization Enables Maintenance of Quiescent Hematopoietic Stem Cells Ex Vivo. Cell Rep. 2019;28(1):145-158.e9. doi:10.1016/j.celrep.2019.06.008.

» View on PubMed

Udden SN, Kwak YT, Godfrey V, et al. NLRP12 suppresses hepatocellular carcinoma via downregulation of cJun N-terminal kinase activation in the hepatocyte. Elife. 2019;8:e40396. Published 2019 Apr 16. doi:10.7554/eLife.40396.

» View on PubMed

Lefort CT, Rossaint J, Moser M, Petrich BG, Zarbock A, Monkley SJ, Critchley DR, Ginsberg MH, Fassler R, and Ley K. 2012. Blood. 119:4275-4282. (in vitro blocking)

Grewal JS, Pilgrim MJ, Grewal S, Kasman L, Werner P, Bruorton ME, London SD, and London L. 2011. FASEB J. 25:1680-1696. (Immunofluorescence microscopy – frozen tissue)

Kim W-K, Sun Y, Do H, Autissier P, Halpern EF, Piatak M, Lifson JD, Burdo TH, McGrath MS, and Williams K. 2010. J. Leukoc. Biol. 87: 557-567. (Flow Cytometry – Rhesus macaque)

Roland CL, Dineen SP, Lynn KD, Sullivan LA, Dellinger MT, Sadegh L, Sullivan JP, Shames DS, and Brekken RA. 2009. Mol. Cancer Ther. 8:1761-1771. (Immunofluorescence microscopy – frozen tissue)

Sorg H, Lorch B, Jaster R, Fitzner B, Ibrahim S, Holzhueter S, Nizze H, and Vollmar B. 2008. Am. J. Physio. Gastrointest. Liver Physiol. 295: G1274-1280. (Immunohistochemistry - formalin-fixed paraffin embedded tissue)

Kim DD, Miwa T, Kimura Y, Schwendener RA, van Lookeren Campagne M, and Song W-C. 2008. Blood. 112:1109-1119. (in vivo blocking)

Ou R, Zhang M, Huang L, Flavell RA, Koni PA, and Moskophidis D. 2008. J. Virol. 82:2952-2965. (Immunohistochemistry – OCT embedded frozen tissue)

Nutt SL, Metcalf D, D’Amico A, Polli M, and Wu L. 2005. J. Exp. Med. 201:221-231. (Immunomagnetic bead depletion)

Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, and Hill TJ. 1995. J. Histochem. Cytochem. 43:313-320. (Immunohistochemistry – frozen tissue, paraffin embedded tissue)

Miller LJ, Schwarting R, and Springer TA. 1986. J. Immunol. 137:2891-2900. (Immunoprecipitation)

Specific References:
Abe Y, Kamachi F, Kawamoto T, Makino F, Ito J, Kojima Y, Moustapha AEDH, Usui Y, Yagita H, Takasaki Y, Okumura K and Akiba H. 2013. J. Immunol. 191:4562-4572. (Flow Cytometry)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.