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C57Bl/6 bone marrow cells were stained with 0.25 ug PE-Cy7 Anti-Mouse Ly-6G (60-5931) (solid line) or 0.25 ug PE-Cy7 Rat IgG2b isotype control (dashed line).
  • C57Bl/6 bone marrow cells were stained with 0.25 ug PE-Cy7 Anti-Mouse Ly-6G (60-5931) (solid line) or 0.25 ug PE-Cy7 Rat IgG2b isotype control (dashed line).
  • PE-Cyanine7 Anti-Mouse Ly-6G (Gr-1) (RB6-8C5)

PE-Cyanine7 Anti-Mouse Ly-6G (Gr-1) (RB6-8C5)

Cat No. Size Price Quantity
60-5931-U025 25 µg $51.00
60-5931-U100 100 µg $113.00

Description

The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.

Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.

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The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.

Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.

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Product Details

Name PE-Cyanine7 Anti-Mouse Ly-6G (Gr-1) (RB6-8C5)
Cat. No. 60-5931
Alternative Names Gr1, Ly6G
Gene ID 17067
Clone RB6-8C5
Isotype Rat IgG2b, κ
Reactivity Mouse
Cross Reactivity
Format PE-Cyanine7
Application Flow Cytometry
Citations*

Hiroshi Kobayashi and Keiyo Takubo. Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells. STAR Protocols.  Published 2 August 2020. https://doi.org/10.1016/j.xpro.2020.100078. 

» View on ScienceDirect

Alkhani A, Levy CS, Tsui M, Rosenberg KA, Polovina K, Mattis AN, Mack M, Van Dyken S, Wang BM, Maher JJ, Nijagal A. Ly6cLo non-classical monocytes promote resolution of rhesus rotavirus-mediated perinatal hepatic inflammation. Sci Rep. 2020 Apr 28;10(1):7165. doi: 10.1038/s41598-020-64158-2.

» View on PubMed

Kobayashi H, Morikawa T, Okinaga A, et al. Environmental Optimization Enables Maintenance of Quiescent Hematopoietic Stem Cells Ex Vivo. Cell Rep. 2019;28(1):145-158.e9. doi:10.1016/j.celrep.2019.06.008.

» View on PubMed

Berent-Maoz B, Montecino-Rodriguez E, Signer RAJ, and Dorshkind K. 2012. Blood. 199:5715-5721. (Flow cytometry)

von Bruhl M-L, Stark K, Steinhart A, et al. 2012. J. Exp. Med. 209: 819-835. (Intravital fluorescent microscopy - video)

Le HT, Tran VG, Kim W, Kim H, Cho HR, and Kwon B. 2012. J. Immunol. 189:287-295. (in vivo neutrophil depletion)

Doring Y, Soehnlein O, Drechsler M, Shagdarsuren E, Chaudhari SM, Meiler S, Hartwig H, Hristov M, Koenen RR, Hieronymus T, Zenke M, Weber C, and Zernecke A. 2012. Arterioscler. Thromb. Vasc. Biol. 32: 1613-1623. (in vivo depletion)

Hickman HD, Li L, Reynoso GV, Rubin EJ, Skon CN, Mays JW, Gibbs J, Schwartz O, Bennink JR, and Yewdell JW. 2011. J. Exp. Med. 208: 2511-2524. (Immunohistochemistry – OCT embedded frozen tissue)

Wang T, Tian L, Haino M, Gao J-L, Lake R, Ward Y, Wang H, Siebenlist U, Murphy PM, and Kelly K. 2007. Infect. Immun. 75(3):1144-1153. (Immunohistochemistry – zinc fixed tissue)

Nutt SL, Metcalf D, D’Amico A, Polli M, and Wu L. 2005. J. Exp. Med. 201:221-231. (Immunomagnetic bead depletion)

Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, and Hill TJ. 1995. J. Histochem. Cytochem. 43:313-320. (Immunohistochemistry – frozen tissue, paraffin embedded tissue)

Fleming TJ, Fleming ML, and Malek TR. 1993. J. Immunol. 151:2399-2408. (in vitro blocking, immunoprecipitation)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.