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C57Bl/6 bone marrow cells were stained with APC Anti-Mouse CD11b (20-0112) and 0.25 ug PE-Cyanine7 Anti-Mouse F4/80 Antigen (60-4801) (right panel) or 0.25 ug PE-Cyanine7 Rat IgG2b isotype control (left panel).
  • C57Bl/6 bone marrow cells were stained with APC Anti-Mouse CD11b (20-0112) and 0.25 ug PE-Cyanine7 Anti-Mouse F4/80 Antigen (60-4801) (right panel) or 0.25 ug PE-Cyanine7 Rat IgG2b isotype control (left panel).

PE-Cyanine7 Anti-Mouse F4/80 Antigen (BM8.1)

Cat No. Size Price Quantity
60-4801-U025 25 µg $85.00
60-4801-U100 100 µg $265.00

Description

The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

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The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

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Product Details

Name PE-Cyanine7 Anti-Mouse F4/80 Antigen (BM8.1)
Cat. No. 60-4801
Alternative Names EMR1, Ly71
Gene ID 13733
Clone BM8.1
Isotype Rat IgG2b, κ
Reactivity Mouse
Cross Reactivity
Format PE-Cyanine7
Application Flow Cytometry
Citations*

Palacios M, Miner TA, Frederick DR, et al. Identification of Two Regulators of Virulence That Are Conserved in Klebsiella pneumoniae Classical and Hypervirulent Strains. mBio. 2018;9(4):e01443-18. Published 2018 Aug 7. doi:10.1128/mBio.01443-18.

» View on PubMed

Ioannou M, Alissafi T, Boon L, Boumpas D, and Verginis P. 2013. J. Immunol. 190: 2631-2640. (Flow Cytometry)

Papadopoulos G, Weinberg EO, Massari P, Gibson FC, Wetzler LM, Morgan EF, and Genco CA. 2013. J. Immunol. 190: 1148-1157.

Chen Q and Snapper CM. 2013. J. Immunol. 190: 1048-1055.

Rankin AL, Mumm JB, Murphy E, Turner S, Yu N, McClanahan TK, Bourne PA, Pierce RH, Kastelein R and Pflanz S. 2010. J. Immunol. 184(3): 1526-1535. (Immunohistochemistry - paraffin-embedded tissue)

Geutskens SB, Otonkoski T, Pulkkinen MA, Drexhage HA and Leenen PJ. 2005. J. Leukoc. Biol. 78(4): 845-52 (Immunohistochemistry - frozen tissue)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.