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C57Bl/6 splenocytes were stained with FITC Anti-Mouse CD4 (35-0041), APC Anti-Human/Mouse Foxp3 (20-5773) and 0.25 ug PE Anti-Mouse TIGIT (50-1421) (right panel) or 0.25 ug PE Mouse IgG1 (left panel).
  • C57Bl/6 splenocytes were stained with FITC Anti-Mouse CD4 (35-0041), APC Anti-Human/Mouse Foxp3 (20-5773) and 0.25 ug PE Anti-Mouse TIGIT (50-1421) (right panel) or 0.25 ug PE Mouse IgG1 (left panel).
  • PE Anti-Mouse TIGIT (1G9)

PE Anti-Mouse TIGIT (1G9)

Cat No. Size Price Quantity
50-1421-U025 25 µg $69.00
50-1421-U100 100 µg $168.00

Description

The 1G9 antibody reacts with mouse TIGIT (T cell Ig and ITIM domain), a 26 kDa member of the CD28 receptor family which is reported to regulate T cell receptor (TCR) activation. Within the CD28 family of receptors there are those which have co-stimulatory activity, such as CD28 and CTLA-4, as well as more recently identified receptors like TIGIT which are proposed to provide co-inhibitory signals. TIGIT is expressed and upregulated on activated T cells, and is also expressed on memory and regulatory T cells. Upon engagement by its ligands, CD112 and CD155, TIGIT signaling inhibits T cell proliferation and suppresses T cell responses, without triggering cell deletion. A second inhibitory effect of TIGIT signaling is the generation of immunoregulatory dendritic cells, which secrete IL-10 and TGF-beta to further inhibit T cell function.

The 1G9 antibody may be used for flow cytometric analysis of TIGIT, which is expressed at very high levels on T regulatory cells (Tregs) and activated conventional T cells, as well as memory T cells and NK cells.

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The 1G9 antibody reacts with mouse TIGIT (T cell Ig and ITIM domain), a 26 kDa member of the CD28 receptor family which is reported to regulate T cell receptor (TCR) activation. Within the CD28 family of receptors there are those which have co-stimulatory activity, such as CD28 and CTLA-4, as well as more recently identified receptors like TIGIT which are proposed to provide co-inhibitory signals. TIGIT is expressed and upregulated on activated T cells, and is also expressed on memory and regulatory T cells. Upon engagement by its ligands, CD112 and CD155, TIGIT signaling inhibits T cell proliferation and suppresses T cell responses, without triggering cell deletion. A second inhibitory effect of TIGIT signaling is the generation of immunoregulatory dendritic cells, which secrete IL-10 and TGF-beta to further inhibit T cell function.

The 1G9 antibody may be used for flow cytometric analysis of TIGIT, which is expressed at very high levels on T regulatory cells (Tregs) and activated conventional T cells, as well as memory T cells and NK cells.

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Product Details

Name PE Anti-Mouse TIGIT (1G9)
Cat. No. 50-1421
Alternative Names VSTM3
Gene ID 100043314
Clone 1G9
Isotype Mouse IgG1, kappa
Reactivity Mouse
Cross Reactivity
Format PE
Application Flow Cytometry
Citations*

Joller N, Peters A, Anderson AC, and Kuchroo VK. 2012. Immunol. Rev. 248(1):122-139. (Flow cytometry)

Joller N, Hafler JP, Brynedal B, Kassam N, Spoerl S, Levin SD, Sharpe AH, and Kuchroo VK. 2011. J. Immunol. 186: 1338-1342. (Flow cytometry)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.