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Mouse macrophages were stimulated in the presence of a protein transport inhibitor. Cells were then fixed, permeabilized and stained intracellularly with 0.125 ug PE Anti-Mouse IL-12/IL-23 p40 (50-7123) (left panel) or 0.125 ug PE Rat IgG2a (left panel).
  • Mouse macrophages were stimulated in the presence of a protein transport inhibitor. Cells were then fixed, permeabilized and stained intracellularly with 0.125 ug PE Anti-Mouse IL-12/IL-23 p40 (50-7123) (left panel) or 0.125 ug PE Rat IgG2a (left panel).
  • PE Anti-Mouse IL-12/IL-23 p40 (C17.8)

PE Anti-Mouse IL-12/IL-23 p40 (C17.8)

Cat No. Size Price Quantity
50-7123-U025 25 µg $58.00
50-7123-U100 100 µg $162.00

Description

The C17.8 antibody is specific for the 40 kDa (p40) protein subunit shared by the cytokines IL-12 and IL-23. To form IL-12, p40 assembles with a separate 35 kDa protein known as p35, resulting in a 70 kDa functional cytokine. IL-12 is secreted by activated monocytes, macrophages, and dendritic cells, and has been shown to target naïve, resting CD4+ T cells to promote their proliferation and secretion of cytokines. IL-23 contains the p40 subunit in combination with a 19 kDa protein chain, p19; its primary source being activated dendritic cells and other antigen-presenting cells. IL-23 appears to target different cell types than IL-12, acting on memory CD4+ T cells to induce a strong proliferative response and contributing to the generation and expansion of Th17 cells. Like the cytokines themselves, the receptors for IL-12 and IL-23 share one subunit, as well as containing distinct cytokine-specific subunits.

As the C17.8 antibody binds to a shared subunit of both IL-12 and IL-23, it may be used as a marker for either IL-12 or IL-23 expression in dendritic cells, monocytes and macrophages, and is widely used for neutralization of activity associated with either cytokine. Please choose the appropriate format for each application.

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The C17.8 antibody is specific for the 40 kDa (p40) protein subunit shared by the cytokines IL-12 and IL-23. To form IL-12, p40 assembles with a separate 35 kDa protein known as p35, resulting in a 70 kDa functional cytokine. IL-12 is secreted by activated monocytes, macrophages, and dendritic cells, and has been shown to target naïve, resting CD4+ T cells to promote their proliferation and secretion of cytokines. IL-23 contains the p40 subunit in combination with a 19 kDa protein chain, p19; its primary source being activated dendritic cells and other antigen-presenting cells. IL-23 appears to target different cell types than IL-12, acting on memory CD4+ T cells to induce a strong proliferative response and contributing to the generation and expansion of Th17 cells. Like the cytokines themselves, the receptors for IL-12 and IL-23 share one subunit, as well as containing distinct cytokine-specific subunits.

As the C17.8 antibody binds to a shared subunit of both IL-12 and IL-23, it may be used as a marker for either IL-12 or IL-23 expression in dendritic cells, monocytes and macrophages, and is widely used for neutralization of activity associated with either cytokine. Please choose the appropriate format for each application.

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Product Details

Name PE Anti-Mouse IL-12/IL-23 p40 (C17.8)
Cat. No. 50-7123
Alternative Names Interleukin-12, IL12 / Interleukin-23, IL23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
Gene ID 16160
Clone C17.8
Isotype Rat IgG2a, κ
Reactivity Mouse
Cross Reactivity
Format PE
Application Flow Cytometry
Citations*

Dolina JS, Lee J, Griswold RQ, Labarta-Bajo L, Kannan S, Greenbaum JA, Bahia El Idrissi N, Pont MJ, Croft M, Schoenberger SP. TLR9 Sensing of Self-DNA Controls Cell-Mediated Immunity to Listeria Infection via Rapid Conversion of Conventional CD4+ T Cells to Treg. Cell Rep. 2020 Apr 7;31(1):107249. doi: 10.1016/j.celrep.2020.01.040.

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Dong H, Franklin NA, Roberts DJ, Yagita H, Glennie MJ and Bullock TNJ. 2012. J. Immunol. 188: 3829-3838. (in vivo blocking)

Prabhakara R, Harro JM, Leid JG, Keegan AD, Prior ML, and Shirtliff ME. 2011. Infect. Immun. 79: 5010-5018. (in vivo blocking)

Chmielewski M, Kopecky C, Hombach AA, and Abken H. 2011. Cancer Res. 71: 5697-5706. (ELISA)

Lo C-H, Lee S-C, Wu P-Y, Pan W-Y et al. 2003. J. Immunol. 171: 600-607. (Immunoprecipitation)

Belladonna ML, Renauld J-C, Bianchi R, Vacca C, Fallarino F, Orabona C, Fioretti MC, Grohmann, and Puccetti P. 2002. J. Immunol. 168: 5448-5454. (Western Blot)

Ludviksson BR, Ehrhardt RO, and Strober W. 1999. J. Immunol. 163: 4349-4359. (Immunofluorescence microscopy – frozen tissue)

Kato K, Shimozato O, Hoshi K, Wakimoto H, Hamada H, Yagita H, and Okumura K. 1996. Proc. Natl. Acad. Sci. 93:9085-9089. (Immunoprecipitation; ELISA)

Wysocka M, Kubin M, Vieira LQ, Ozmen L, Garotta G, Scott P and Trinchieri G. 1995. Eur. J. Immunol. 25: 672-676. (Origination of clone; Immunoprecipitation, Western Blot)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.