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C57Bl/6 bone marrow cells were stained with APC Anti-Mouse CD11b (20-0112) and 0.25 ug PE Anti-Mouse F4/80 Antigen (50-4801) (right panel) or 0.25 ug PE Rat IgG2b isotype control (left panel).
  • C57Bl/6 bone marrow cells were stained with APC Anti-Mouse CD11b (20-0112) and 0.25 ug PE Anti-Mouse F4/80 Antigen (50-4801) (right panel) or 0.25 ug PE Rat IgG2b isotype control (left panel).

PE Anti-Mouse F4/80 Antigen (BM8.1)

Cat No. Size Price Quantity
50-4801-U025 25 µg $45.00
50-4801-U100 100 µg $141.00

Description

The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

Recent Publications:

Ishida Y, Kuninaka Y, Nosaka M, et al. Prevention of CaCl2-induced aortic inflammation and subsequent aneurysm formation by the CCL3-CCR5 axis. Nat Commun. 2020 Nov 25;11(1):5994. doi: 10.1038/s41467-020-19763-0. PMID: 33239616.

» View on Nature Communications 

Saeki N, Imai Y. Reprogramming of synovial macrophage metabolism by synovial fibroblasts under inflammatory conditions. Cell Commun Signal. 2020 Nov 30;18(1):188. doi: 10.1186/s12964-020-00678-8. PMID: 33256735; PMCID: PMC7708128. 

» View on PubMed

Wang H, Najibi AJ, Sobral MC, et al. Biomaterial-based scaffold for in situ chemo-immunotherapy to treat poorly immunogenic tumors. Nat Commun. 2020 Nov 10;11(1):5696. doi: 10.1038/s41467-020-19540-z. PMID: 33173046; PMCID: PMC7655953.

» View on PubMed

Sardari M, Skuljec J, Yin D, et al. Lipopolysaccharide-induced sepsis-like state compromises post-ischemic neurological recovery, brain tissue survival and remodeling via mechanisms involving microvascular thrombosis and brain T cell infiltration. Brain Behav Immun. 2020 Oct 22:S0889-1591(20)32365-5. doi: 10.1016/j.bbi.2020.10.015. PMID: 33122024. 

» View on ScienceDirect

Singh V, Holla S, Ramachandra SG and Balaji KN. 2015.   J. Immunol. doi: 10.4049/jimmunol.1402498 (Flow cytometry)

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The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

Recent Publications:

Ishida Y, Kuninaka Y, Nosaka M, et al. Prevention of CaCl2-induced aortic inflammation and subsequent aneurysm formation by the CCL3-CCR5 axis. Nat Commun. 2020 Nov 25;11(1):5994. doi: 10.1038/s41467-020-19763-0. PMID: 33239616.

» View on Nature Communications 

Saeki N, Imai Y. Reprogramming of synovial macrophage metabolism by synovial fibroblasts under inflammatory conditions. Cell Commun Signal. 2020 Nov 30;18(1):188. doi: 10.1186/s12964-020-00678-8. PMID: 33256735; PMCID: PMC7708128. 

» View on PubMed

Wang H, Najibi AJ, Sobral MC, et al. Biomaterial-based scaffold for in situ chemo-immunotherapy to treat poorly immunogenic tumors. Nat Commun. 2020 Nov 10;11(1):5696. doi: 10.1038/s41467-020-19540-z. PMID: 33173046; PMCID: PMC7655953.

» View on PubMed

Sardari M, Skuljec J, Yin D, et al. Lipopolysaccharide-induced sepsis-like state compromises post-ischemic neurological recovery, brain tissue survival and remodeling via mechanisms involving microvascular thrombosis and brain T cell infiltration. Brain Behav Immun. 2020 Oct 22:S0889-1591(20)32365-5. doi: 10.1016/j.bbi.2020.10.015. PMID: 33122024. 

» View on ScienceDirect

Singh V, Holla S, Ramachandra SG and Balaji KN. 2015.   J. Immunol. doi: 10.4049/jimmunol.1402498 (Flow cytometry)

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Product Details

Name PE Anti-Mouse F4/80 Antigen (BM8.1)
Cat. No. 50-4801
Alternative Names EMR1, Ly71
Gene ID 13733
Clone BM8.1
Isotype Rat IgG2b, κ
Reactivity Mouse
Cross Reactivity
Format PE
Application Flow Cytometry
Citations*

Ioannou M, Alissafi T, Boon L, Boumpas D, and Verginis P. 2013. J. Immunol. 190: 2631-2640. (Flow Cytometry)

Papadopoulos G, Weinberg EO, Massari P, Gibson FC, Wetzler LM, Morgan EF, and Genco CA. 2013. J. Immunol. 190: 1148-1157.

Chen Q and Snapper CM. 2013. J. Immunol. 190: 1048-1055.

Rankin AL, Mumm JB, Murphy E, Turner S, Yu N, McClanahan TK, Bourne PA, Pierce RH, Kastelein R and Pflanz S. 2010. J. Immunol. 184(3): 1526-1535. (Immunohistochemistry - paraffin-embedded tissue)

Geutskens SB, Otonkoski T, Pulkkinen MA, Drexhage HA and Leenen PJ. 2005. J. Leukoc. Biol. 78(4): 845-52 (Immunohistochemistry - frozen tissue)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.