Customer Service: (855) 848-6626 | 8:00 a.m - 4:30 p.m. PST | Receive a Free Mask with Orders over $300

C57Bl/6 splenocytes were unstimulated (left panel) or stimulated for 3 days with LPS (right panel) and stained with 0.125 ug PE Anti-Mouse CD86 (50-0861) (solid line) or 0.125 ug PE Rat IgG2b isotype control (dashed line).
  • C57Bl/6 splenocytes were unstimulated (left panel) or stimulated for 3 days with LPS (right panel) and stained with 0.125 ug PE Anti-Mouse CD86 (50-0861) (solid line) or 0.125 ug PE Rat IgG2b isotype control (dashed line).

PE Anti-Mouse CD86 (B7-2) (PO3.1)

Cat No. Size Price Quantity
50-0861-U025 25 µg $40.00
50-0861-U100 100 µg $118.00

Description

The PO3.1 antibody reacts with mouse CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between ≥ ≥ ≥ the B7 ligands and CD28 provides crucial communication between ≥ ≥ ≥ T cells and B cells or APCs to coordinate the adaptive immune response.

The PO3.1 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.

View More

The PO3.1 antibody reacts with mouse CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between ≥ ≥ ≥ the B7 ligands and CD28 provides crucial communication between ≥ ≥ ≥ T cells and B cells or APCs to coordinate the adaptive immune response.

The PO3.1 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.

Hide

Product Details

Name PE Anti-Mouse CD86 (B7-2) (PO3.1)
Cat. No. 50-0861
Alternative Names B7.2, B70, Ly-58
Gene ID 12524
Clone PO3.1
Isotype Rat IgG2b, κ
Reactivity Mouse
Cross Reactivity
Format PE
Application Flow Cytometry
Citations*

Ioannou M, Alissafi T, Lazaridis I, Deraos G, Matsoukas J, Gravanis A, Mastorodemos V, Plaitakis A, Sharpe A, Boumpas D, and Vergiinis P. 2012. J. Immunol. 188: 1136-1146. (Flow cytometry)

Zhang J, Kawashima N, Suda H, Nakano Y, Takano Y, and Azuma M. 2006. Int. Immunol. 18: 1375-1384. (Immunohistochemistry – frozen tissue)

Kin NW and Sanders VM. 2006. J. Immunol. 176: 6727 – 6735. (in vitro activation)

Spadaro M, Ambrosino E, Iezzi M, Di Carlo E, Sacchetti P, Curcio C, Amici A, Wei W-Z, Musiani P, Lollini P-L, Cavallo F, Forni G. 2005. Clin. Cancer Res. 11: 1941-1952. (Immunohistochemistry – frozen tissue)

Pokojil JR< Kin Nw, and Sanders VM. 2004. 279: 23394-23404. (in vitro activation)

Iwai H, Kozono Y, Hirose S, Akiba H, Yagita H, Okumura K, Kohsaka H, Miyasaka N, and Azuma M. 2002. J. Immunol. 169: 4332-4339. (in vitro activation)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.