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C57Bl/6 splenocytes were stained with 0.5 ug PE Anti-Mouse CD45.2 (50-0454) (solid line) or 0.5 ug PE Mouse IgG2a isotype control (dashed line).
  • C57Bl/6 splenocytes were stained with 0.5 ug PE Anti-Mouse CD45.2 (50-0454) (solid line) or 0.5 ug PE Mouse IgG2a isotype control (dashed line).

PE Anti-Mouse CD45.2 (104)

Cat No. Size Price Quantity
50-0454-U025 25 µg $41.00
50-0454-U100 100 µg $110.00

Description

The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.

The 104 antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to be specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells.

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The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.

The 104 antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to be specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells.

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Product Details

Name PE Anti-Mouse CD45.2 (104)
Cat. No. 50-0454
Alternative Names Ly5.2
Gene ID 19264
Clone 104
Isotype Mouse IgG2a, κ
Reactivity Mouse
Cross Reactivity
Format PE
Application Flow Cytometry
Citations*

Scharer CD, Patterson DG, Mi T, Price MJ, Hicks SL, Boss JM. Antibody-secreting cell destiny emerges during the initial stages of B-cell activation. Nat Commun. 2020;11(1):3989. Published 2020 Aug 10. doi:10.1038/s41467-020-17798-x.

» View on Nature Communications

Willinger T and Flavell, RA. 2012. Proc. Natl. Acad. Sci. 109: 8670 - 8675. (Flow Cytometry)

Hale JS, Nelson LT, Simmons KB, and Fink PJ. 2011. J. Immunol. 186: 799 - 806. (Flow Cytometry)

Orr MT, Beilke JN, Proekt I, and Lanier LL. 2010. Proc. Natl. Acad. Sci. 107: 15844 - 15849. (Flow Cytometry)

Banerjee K, Biswas PS, Kumaraguru U, Schoenberger SP, and Rouse BT. 2004. 173: 7575-7583. (Immunofluorescence microscopy – frozen tissue)

Favre CJ, Mancuso M, Maas K, McLean JW, Baluk P, and Mcdonald DM. 2003. Am. J. Physiol. Heart Circ. Physiol. 285:H1917-H1938. (Immunocytochemistry)

Shen F-W, Tung J-S, and Boyse EA. 1986. Immunogenetics. 24(3): 146-149. (Immunoprecipitation).

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.