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C57Bl/6 spleen cells were stained with APC Anti-Mouse CD3 (20-0031) and 0.06 ug PE Anti-Mouse CD275 (50-5985) (right panel) or 0.06 ug PE Rat IgG2a isotype control (left panel).
  • C57Bl/6 spleen cells were stained with APC Anti-Mouse CD3 (20-0031) and 0.06 ug PE Anti-Mouse CD275 (50-5985) (right panel) or 0.06 ug PE Rat IgG2a isotype control (left panel).

PE Anti-Mouse CD275 (B7-H2) (HK5.3)

Cat No. Size Price Quantity
50-5985-U025 25 µg $54.00
50-5985-U100 100 µg $209.00

Description

The HK5.3 antibody reacts with mouse CD275, an approximately 40 kDa protein that is a member of the Ig superfamily. CD275 is also known as B7-H2, ICOS ligand, and B7-RP1. CD275 is a ligand for ICOS (CD278) that is expressed on activated T cells. CD275 is expressed by antigen presenting cells including B cells, macrophages, and dendritic cells. The interaction of CD275 with its receptor ICOS plays an important role in T cell costimulation.

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The HK5.3 antibody reacts with mouse CD275, an approximately 40 kDa protein that is a member of the Ig superfamily. CD275 is also known as B7-H2, ICOS ligand, and B7-RP1. CD275 is a ligand for ICOS (CD278) that is expressed on activated T cells. CD275 is expressed by antigen presenting cells including B cells, macrophages, and dendritic cells. The interaction of CD275 with its receptor ICOS plays an important role in T cell costimulation.

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Product Details

Name PE Anti-Mouse CD275 (B7-H2) (HK5.3)
Cat. No. 50-5985
Alternative Names B7h, B7-H2, GL50, ICOS Ligand
Gene ID 50723
Clone HK5.3
Isotype Rat IgG2a, κ
Reactivity Mouse
Cross Reactivity
Format PE
Application Flow Cytometry
Citations*

Yoshinaga SK, Whoriskey JS, Khare SD, Sarmiento U, Guo J, Horan T, Shih G, Zhang M, Coccia MA, Kohno T, Tafuri-Bladt A, Brankow D, Campbell P, Chang D, Chiu L, Dai T, Duncan G, Elliott GS, Hui A, McCabe SM, Scully S, Shahinian A, Shaklee CL, Van G, Mak TW, Senaldi G. 1999. Nature. Dec 16;402(6763):827-832.

Ling V, Wu PW, Finnerty HF, Bean KM, Spaulding V, Fouser LA, Leonard JP, Hunter SE, Zollner R, Thomas JL, Miyashiro JS, Jacobs KA, Collins M. 2000. J Immunol. Feb 15;164(4):1653-1657.

Iwai H, Abe M, Hirose S, Tsushima F, Tezuka K, Akiba H, Yagita H, Okumura K, Kohsaka H, Miyasaka N, Azuma M. 2003. J Immunol. Sep 15;171(6):2848-2854.

Kohyama M1, Sugahara D, Sugiyama S, Yagita H, Okumura K, Hozumi N. 2004. Proc Natl Acad Sci USA. Mar 23;101(12):4192-4197.

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.