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C57Bl/6 splenocytes were stained with 0.125 ug PE Anti-Mouse CD19 (50-0193) (solid line) or 0.125 ug PE Rat IgG2a isotype control (dashed line).
  • C57Bl/6 splenocytes were stained with 0.125 ug PE Anti-Mouse CD19 (50-0193) (solid line) or 0.125 ug PE Rat IgG2a isotype control (dashed line).

PE Anti-Mouse CD19 (1D3)

Cat No. Size Price Quantity
50-0193-U025 25 µg $45.00
50-0193-U100 100 µg $74.00

Description

The 1D3 antibody reacts with mouse CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21 and CD81, in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells. The 1D3 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.

Recent Publications:

Weber A, Pawar HN, Klein JR. Thyroid stimulating hormone β-subunit splice variant is expressed in all fractional subsets of bone marrow hematopoietic cells and peripheral blood leukocytes and is modulated during bacterial infection. Gen Comp Endocrinol. 2020 Apr 30:113495. doi: 10.1016/j.ygcen.2020.113495

» View on PubMed

Qiumei Du, Ashley R. Hoover, Igor Dozmorov, Ondine B. Cleaver,Joshua T. Mendell, Nicolai S.C. van Oers. MIR205HG Is a Long Noncoding RNA that Regulates Growth Hormone and Prolactin Production in the Anterior Pituitary. Dev Cell. 2019 May 20;49(4):618-631.e5. doi: 10.1016/j.devcel.2019.03.012

» View on PubMed

Ortega SB, Noorbhai I, Poinsatte K, Kong X, Anderson A, Monson NL and Stowe AM. 2015. Discov Med. 19(106): 381-392. (Flow Cytometry)

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The 1D3 antibody reacts with mouse CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21 and CD81, in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells. The 1D3 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.

Recent Publications:

Weber A, Pawar HN, Klein JR. Thyroid stimulating hormone β-subunit splice variant is expressed in all fractional subsets of bone marrow hematopoietic cells and peripheral blood leukocytes and is modulated during bacterial infection. Gen Comp Endocrinol. 2020 Apr 30:113495. doi: 10.1016/j.ygcen.2020.113495

» View on PubMed

Qiumei Du, Ashley R. Hoover, Igor Dozmorov, Ondine B. Cleaver,Joshua T. Mendell, Nicolai S.C. van Oers. MIR205HG Is a Long Noncoding RNA that Regulates Growth Hormone and Prolactin Production in the Anterior Pituitary. Dev Cell. 2019 May 20;49(4):618-631.e5. doi: 10.1016/j.devcel.2019.03.012

» View on PubMed

Ortega SB, Noorbhai I, Poinsatte K, Kong X, Anderson A, Monson NL and Stowe AM. 2015. Discov Med. 19(106): 381-392. (Flow Cytometry)

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Product Details

Name PE Anti-Mouse CD19 (1D3)
Cat. No. 50-0193
Alternative Names Leu-12, B4
Gene ID 12478
Clone 1D3
Isotype Rat IgG2a, κ
Reactivity Mouse
Cross Reactivity
Format PE
Application Flow Cytometry
Citations*

Weber A, Pawar HN, Klein JR. Thyroid stimulating hormone β-subunit splice variant is expressed in all fractional subsets of bone marrow hematopoietic cells and peripheral blood leukocytes and is modulated during bacterial infection. Gen Comp Endocrinol. 2020 Apr 30:113495. doi: 10.1016/j.ygcen.2020.113495

» View on PubMed

Qiumei Du, Ashley R. Hoover, Igor Dozmorov, Ondine B. Cleaver,Joshua T. Mendell, Nicolai S.C. van Oers. MIR205HG Is a Long Noncoding RNA that Regulates Growth Hormone and Prolactin Production in the Anterior Pituitary. Dev Cell. 2019 May 20;49(4):618-631.e5. doi: 10.1016/j.devcel.2019.03.012

» View on PubMed

Ghosn EEB, Yamamoto R, Hamanada S, Yang Y, Herzenberg LA, Nakauchi H, and Herzenberg LA. 2012. Proc. Natl. Acad. Sci. 109:5394-5398. (Flow cytometry)

Raghavan S, Ostberg AK, Flach C-F, Ekman A, Blomquist M, Czerkinsky C, and Holmgren J. 2010. Infect. Immun. 78(10)4251-4260. (Immunohistochemistry – acetone fixed tissue)

Togayachi A, Kozono Y, Ikehara Y, Ito H, et al. 2010. Proc. Natl. Acad. Sci. 107:11900-11905. (Immunoprecipitation)

Poitrasson-Riviere M, Bienvenu B, Le Campion A, Becourt C, Martin B, and Lucas B. 2008. J. Immunol. 180:7294-7304. (Immunohistochemistry – frozen tissue)

Lee Y, Haas KM, Gor DO, Ding X, Karp DR, Greenspan NS, Poe JC, and Tedder TF. 2005. J. Immunol. 175:8011-8023. (Immunoprecipitation)

Bobbitt KR and Justement LB. 2000. J. Immunol. 165: 5588-5596. (in vitro stimulation, Immunoprecipitation)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.