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C57Bl/6 peripheral blood cells were stained with APC Anti-Mouse CD11b (20-0112) and 0.125 ug PE Anti-Mouse CD115  (50-1152) (right panel) or 0.125 ug PE Rat IgG2a (left panel).
  • C57Bl/6 peripheral blood cells were stained with APC Anti-Mouse CD11b (20-0112) and 0.125 ug PE Anti-Mouse CD115  (50-1152) (right panel) or 0.125 ug PE Rat IgG2a (left panel).

PE Anti-Mouse CD115 (c-fms) (AFS98)

Cat No. Size Price Quantity
50-1152-U025 25 µg $58.00
50-1152-U100 100 µg $124.00

Description

The AFS98 antibody is specific for mouse CD115, also known as Colony-Stimulating Factor-1 Receptor (CSF-1R), a 145 kDa receptor from the PDGF receptor family. Receptor activation by the ligands IL-34 or CSF-1 (M-CSF) occurs via homodimerization of CD115 and subsequent tyrosine phosphorylation and ubiquitination of intracellular domains. CD115 signaling promotes differentiation of myeloid precursors, as well as the continued regulation of proliferation, survival and function of mononuclear phagocytes, dendritic cells and osteoclasts. While IL-34 and CSF-1 may induce similar cellular responses, they are differentially expressed and as such exert complimentary actions via CD115.

The AFS98 antibody may be used for identification of myeloid lineage cells by flow cytometry, and is commonly used for in vivo or in vitro neutralization of CSF-1 Receptor (use format appropriate for functional assays).

Recent Publications:
Grajales-Reyes GE, Iwata A, Albring J, Wu X, Tussiwand R, Kc W, Kretzer NM, Briseno CG, Durai V, Bagadia P, Haldar M, Schonheit J, Rosenbauer F, Murphy TL and Murphy KM. 2015.  Nat Immunol. 16(7): 708-717. (Flow Cytometry)

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The AFS98 antibody is specific for mouse CD115, also known as Colony-Stimulating Factor-1 Receptor (CSF-1R), a 145 kDa receptor from the PDGF receptor family. Receptor activation by the ligands IL-34 or CSF-1 (M-CSF) occurs via homodimerization of CD115 and subsequent tyrosine phosphorylation and ubiquitination of intracellular domains. CD115 signaling promotes differentiation of myeloid precursors, as well as the continued regulation of proliferation, survival and function of mononuclear phagocytes, dendritic cells and osteoclasts. While IL-34 and CSF-1 may induce similar cellular responses, they are differentially expressed and as such exert complimentary actions via CD115.

The AFS98 antibody may be used for identification of myeloid lineage cells by flow cytometry, and is commonly used for in vivo or in vitro neutralization of CSF-1 Receptor (use format appropriate for functional assays).

Recent Publications:
Grajales-Reyes GE, Iwata A, Albring J, Wu X, Tussiwand R, Kc W, Kretzer NM, Briseno CG, Durai V, Bagadia P, Haldar M, Schonheit J, Rosenbauer F, Murphy TL and Murphy KM. 2015.  Nat Immunol. 16(7): 708-717. (Flow Cytometry)

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Product Details

Name PE Anti-Mouse CD115 (c-fms) (AFS98)
Cat. No. 50-1152
Alternative Names CSF-1-R, FMS, Colony-Stimulating Factor 1 Receptor, M-CSF Receptor, CSF1R
Gene ID 12978
Clone AFS98
Isotype Rat IgG2a, κ
Reactivity Mouse
Cross Reactivity
Format PE
Application Flow Cytometry
Citations*

Gautier EL, Chow A, Spanbroek R, Marcelin G, Greter M, Jakubzick C, Bogunovic M, Leboeuf M, van Rooijen N, Habenicht AJ, Merad M, and Randolph GJ. 2012. J. Immunol. 189: 2614-2624. (Flow cytometry)

Nakamichi Y, Mizoguchi T, Arai A, Kobayashi Y, Sato M, Penninger JM, Yasuda H, Kato, S, DeLuca HF, Suda T, Udagawa N, and Takahashi N. Proc. Natl. Acad. Sci. 109: 10006-10011. (in vitro blocking)

Okuno Y, Nakamura-Ishizu A, Kishi K, Suda T, and Kubota Y. 2011. Blood. 117: 5264-5272. (in vivo blocking)

Fixley FJ, Xiong Y, Yu R Y-L, Sahai EA, Stanley ER, and Ye BH. 2005. J. Cell Sci. 118: 1873-1883. (Western blot)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.