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Tube 1 enables the identification of lymphocytes, total T cells, helper T cells, cytotoxic T cells, memory and naïve T cells, B cells and NK cells. Cells are first gated on viable cells, singlets, lymphocytes and CD45+. The CD45+ cells are then divided into B cells and non-B cells. T cells are characterized by expression of CD3 and TCRb from the non-B cell gate. T cells are further characterized by expression of CD4, CD8, CD44 and CD62L.
  • Tube 1 enables the identification of lymphocytes, total T cells, helper T cells, cytotoxic T cells, memory and naïve T cells, B cells and NK cells. Cells are first gated on viable cells, singlets, lymphocytes and CD45+. The CD45+ cells are then divided into B cells and non-B cells. T cells are characterized by expression of CD3 and TCRb from the non-B cell gate. T cells are further characterized by expression of CD4, CD8, CD44 and CD62L.
  • Tube 2 enables the identification of the myeloid subsets: monocytes, macrophages, and neutrophils in addition to the dendritic subsets: cDC1, cDC2 and pDC. Cells are first gated on viable cells, singlets and CD45+CD3-. The CD45+CD3- cells are then divided to characterize myeloid lineage subsets by expression of CD11b, Ly6C, Ly6G and F4/80 and DC lineage subsets by expression of CD8, CD11c, B220, Ly6C and CD4.
  • Tube 3 enables the identification of regulatory T cells (Tregs) in addition to immune checkpoint receptors. Cells are first gated on viable cells, singlets and CD45+CD3+. The CD45+CD3+ cells are then sub-gated by expression of CD4, CD8, CD25, FoxP3 and CD69 to identify T cell subsets and Treg subsets. Immune checkpoint receptor expression can be determined by gating on CD223 (Lag-3), CD279 (PD-1) and CD152 (CTLA-4) subsets.

Mouse TBNK/Myeloid/Treg Kit

Cat No. Size Price Quantity
95-K001-T025 25 Test Kit $2,450.00

Description

The TonboTM Mouse TBNK/Myeloid/Treg Kit is designed by Cytek scientists to enable researchers to quickly perform basic immunophenotyping and analyze mouse B cell, T cell, NK cell, Myeloid and Treg cell populations in mouse samples. This pre-titered ready-to-use mouse kit contains three sets of reagents that are fully optimized for best performance on the Cytek Aurora and Northern Lights full spectrum systems.

 

Cytek provides the following tools to streamline the laboratory workflow:

Note: experiment and analysis templates are for use with Cytek® SpectroFlo software version 3.0 or newer

    • Complete protocols for sample preparation, staining, and acquisition

Download Sample Preparation Protocol

    • Experiment and analysis templates to streamline workflow and improve laboratory efficiency.

Download TBNK (Tube 1) Template

Download Myeloid (Tube 2) Template

Download Treg (Tube 3) Template

 

The Mouse TBNK/Myeloid/Treg Kit can also be used as a backbone to expand to a broader panel of your desire.

Data was generated using ViaDye™ Red Fixable Viability Dye


488 640 405
FITC PerCP-Cyanine5.5 PE PE-Cyanine5 PE-Cyanine7 APC redFluor® 710 APC-Cyanine7 violetFluor 450 violetFluor 500 cFluor® V610
Tube 1 Specificity CD8 TCRbeta CD62L CD19 CD44 CD49b CD45R/B220 CD3 NK1.1 CD45 CD4
Clone 53-6.7 H57-597 MEL-14 1D3 IM7 HMa2 RA3-6B2 17A2 PK136 30-F11 RM4-5
Tube 2 Specificity CD8 Ly-6C F4/80 CD3 CD11c CD11b CD45R/B220 I-A/I-E Ly-6G CD45 CD4
Clone 53-6.7 HK1.4 BM8.1 17A2 N418 M1/70 RA3-6B2 M5/114.15.2 1A8 30-F11 RM4-5
Tube 3 Specificity CD8 FOXp3 CD152 CD279 CD25 CD223 CD3 CD69 CD45 CD4
Clone 53-6.7 3G3 UC10-4F10-11 RMP1-30 PC61.5 C9B7W 17A2 H1.2F3 30-F11 RM4-5
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The TonboTM Mouse TBNK/Myeloid/Treg Kit is designed by Cytek scientists to enable researchers to quickly perform basic immunophenotyping and analyze mouse B cell, T cell, NK cell, Myeloid and Treg cell populations in mouse samples. This pre-titered ready-to-use mouse kit contains three sets of reagents that are fully optimized for best performance on the Cytek Aurora and Northern Lights full spectrum systems.

 

Cytek provides the following tools to streamline the laboratory workflow:

Note: experiment and analysis templates are for use with Cytek® SpectroFlo software version 3.0 or newer

    • Complete protocols for sample preparation, staining, and acquisition

Download Sample Preparation Protocol

    • Experiment and analysis templates to streamline workflow and improve laboratory efficiency.

Download TBNK (Tube 1) Template

Download Myeloid (Tube 2) Template

Download Treg (Tube 3) Template

 

The Mouse TBNK/Myeloid/Treg Kit can also be used as a backbone to expand to a broader panel of your desire.

Data was generated using ViaDye™ Red Fixable Viability Dye


488 640 405
FITC PerCP-Cyanine5.5 PE PE-Cyanine5 PE-Cyanine7 APC redFluor® 710 APC-Cyanine7 violetFluor 450 violetFluor 500 cFluor® V610
Tube 1 Specificity CD8 TCRbeta CD62L CD19 CD44 CD49b CD45R/B220 CD3 NK1.1 CD45 CD4
Clone 53-6.7 H57-597 MEL-14 1D3 IM7 HMa2 RA3-6B2 17A2 PK136 30-F11 RM4-5
Tube 2 Specificity CD8 Ly-6C F4/80 CD3 CD11c CD11b CD45R/B220 I-A/I-E Ly-6G CD45 CD4
Clone 53-6.7 HK1.4 BM8.1 17A2 N418 M1/70 RA3-6B2 M5/114.15.2 1A8 30-F11 RM4-5
Tube 3 Specificity CD8 FOXp3 CD152 CD279 CD25 CD223 CD3 CD69 CD45 CD4
Clone 53-6.7 3G3 UC10-4F10-11 RMP1-30 PC61.5 C9B7W 17A2 H1.2F3 30-F11 RM4-5
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Product Details

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.