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Lymphocyte Separation Medium
  • Lymphocyte Separation Medium

Lymphocyte Separation Medium

Cat No. Size Price Quantity
TNB-4700-L100 100 mL $39.00

Description

Lymphocyte Separation Medium (LSM) is a sterile-filtered iso-osmotic solution designed for the in vitro isolation of lymphocytes from diluted whole blood. The use of LSM is based on the method of isolating lymphocytes using density gradient centrifugation (differential centrifugation), resulting in the formation of several distinct cell layers. Mononuclear cells (lymphocytes and monocytes) and platelets will be contained in the banded plasma-LSM interphase due to their density. Erythrocytes and granulocytes will migrate through the gradient to the bottom of the tube. Lymphocytes can be recovered by aspirating the plasma layer and removing the cells.

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Lymphocyte Separation Medium (LSM) is a sterile-filtered iso-osmotic solution designed for the in vitro isolation of lymphocytes from diluted whole blood. The use of LSM is based on the method of isolating lymphocytes using density gradient centrifugation (differential centrifugation), resulting in the formation of several distinct cell layers. Mononuclear cells (lymphocytes and monocytes) and platelets will be contained in the banded plasma-LSM interphase due to their density. Erythrocytes and granulocytes will migrate through the gradient to the bottom of the tube. Lymphocytes can be recovered by aspirating the plasma layer and removing the cells.

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Product Details

Name Lymphocyte Separation Medium
Cat. No. TNB-4700
Protocol Download Protocol
Application
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.