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Gibson Assembly® Positive Control
  • Gibson Assembly® Positive Control

Gibson Assembly® Positive Control

Cat No. Size Price Quantity
GA1000-05C 5rxn $67.00

Description

The positive control consists of a mixture of 10 ng of a 1.5 kb insert and 30 ng of a 2.7 kb vector containing an ampicillin resistance gene. Select for the 4.2 kb assembled construct on LB agar plates with 100 ug/ml ampicillin, 0.1 mM IPTG, and 40 ug/mlL X-Gal. The positive control DNA supplied with this kit is sufficient for 2 reactions (Cat. GA1100-S and GA1100-10) or 5 reactions (Cat. GA1100-50).

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The positive control consists of a mixture of 10 ng of a 1.5 kb insert and 30 ng of a 2.7 kb vector containing an ampicillin resistance gene. Select for the 4.2 kb assembled construct on LB agar plates with 100 ug/ml ampicillin, 0.1 mM IPTG, and 40 ug/mlL X-Gal. The positive control DNA supplied with this kit is sufficient for 2 reactions (Cat. GA1100-S and GA1100-10) or 5 reactions (Cat. GA1100-50).

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Product Details

Name Gibson Assembly® Positive Control
Cat. No. GA1000
Alternative Names
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.