Customer Service: (855) 848-6626 | 8:00 a.m - 4:30 p.m. PST | Save 35% on Ghost Dyes™

Gibson Assembly® HiFi - HC 1-Step Kit
  • Gibson Assembly® HiFi - HC 1-Step Kit

Gibson Assembly® HiFi - HC 1-Step Kit

Cat No. Size Price Quantity
GA1100-4XS  5 rxn $88.00
GA1100-4X10 10 rxn $175.00
GA1100-4X50 50 rxn $700.00

Description

The Gibson Assembly® method can be used to rapidly clone multiple DNA fragments into any vector in one hour or less without the use of restriction enzymes. Instead of relying on the presence of restriction sites, user-defined overlapping ends are incorporated into the fragments to allow the seamless joining of adjacent fragments. By designing DNA fragments with homologous overlapping ends, users of the Gibson Assembly® method can create DNA constructs in a single round of cloning. The method is initiated by combining DNA fragments with the Gibson Assembly® Master Mix. The master mix enzyme cocktail mediates strand chew back, exposing a single strand which allows for annealing of the terminal homologous overlap sequences. Annealing of the homologous overlap sequences is followed by extension and ligation to yield an assembled product. Seamless assembly can be readily applied to both routine cloning and large and complex cloning projects. The 4X Gibson Assembly HiFi HC 1-Step kit requires only 2.5 µL of master mix per 10 µL reaction making it ideal for assemblies using less concentrated DNA samples. Please see the Gibson Assembly® HiFi Manual here.

Quick Reference Manual for Gibson Assembly® HiFi 1-Step HC KitsDownload the Quick Reference Manual for Gibson Assembly® HiFi 1-Step HC Kits.

Instructions Manual for Gibson Assembly® HiFi 1-Step HC KitsDownload the Instructions Manual for Gibson Assembly® HiFi 1-Step HC Kits.

Application Notes for Gibson Assembly® HiFi 1-Step HC KitsDownload the Application Notes for Gibson Assembly® HiFi 1-Step HC Kits.

View More

The Gibson Assembly® method can be used to rapidly clone multiple DNA fragments into any vector in one hour or less without the use of restriction enzymes. Instead of relying on the presence of restriction sites, user-defined overlapping ends are incorporated into the fragments to allow the seamless joining of adjacent fragments. By designing DNA fragments with homologous overlapping ends, users of the Gibson Assembly® method can create DNA constructs in a single round of cloning. The method is initiated by combining DNA fragments with the Gibson Assembly® Master Mix. The master mix enzyme cocktail mediates strand chew back, exposing a single strand which allows for annealing of the terminal homologous overlap sequences. Annealing of the homologous overlap sequences is followed by extension and ligation to yield an assembled product. Seamless assembly can be readily applied to both routine cloning and large and complex cloning projects. The 4X Gibson Assembly HiFi HC 1-Step kit requires only 2.5 µL of master mix per 10 µL reaction making it ideal for assemblies using less concentrated DNA samples. Please see the Gibson Assembly® HiFi Manual here.

Quick Reference Manual for Gibson Assembly® HiFi 1-Step HC KitsDownload the Quick Reference Manual for Gibson Assembly® HiFi 1-Step HC Kits.

Instructions Manual for Gibson Assembly® HiFi 1-Step HC KitsDownload the Instructions Manual for Gibson Assembly® HiFi 1-Step HC Kits.

Application Notes for Gibson Assembly® HiFi 1-Step HC KitsDownload the Application Notes for Gibson Assembly® HiFi 1-Step HC Kits.

Hide

Product Details

Name Gibson Assembly® HiFi - HC 1-Step Kit
Cat. No. GA1100-HC
Alternative Names
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.