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Mouse bone marrow cells heat killed at 65°C for 10 minutes and then mixed with live mouse bone marrow cells. Cells were then stained with Ghost Violet 540. Viable gate is indicated.
  • Mouse bone marrow cells heat killed at 65°C for 10 minutes and then mixed with live mouse bone marrow cells. Cells were then stained with Ghost Violet 540. Viable gate is indicated.

Ghost Dye™ Violet 540

Cat No. Size Price Quantity
13-0879-T100 100 tests $44.00
13-0879-T500 500 tests (5 x 100 tests) $170.00

Description

Ghost Dye™ Violet 540 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Violet 540 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.
Recent Citations:

Tapanes SA, Arizanovska D, Díaz MM, Folorunso OO, Harvey T, Brown SE, Radzishevsky I, Close LN, Jagid JR, Graciolli Cordeiro J, Wolosker H, Balu DT, Liebl DJ. Inhibition of glial D-serine release rescues synaptic damage after brain injury. Glia. 2022 Feb 23. doi: 10.1002/glia.24161. Epub ahead of print. PMID: 35195906.

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Ghost Dye™ Violet 540 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Violet 540 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.
Recent Citations:

Tapanes SA, Arizanovska D, Díaz MM, Folorunso OO, Harvey T, Brown SE, Radzishevsky I, Close LN, Jagid JR, Graciolli Cordeiro J, Wolosker H, Balu DT, Liebl DJ. Inhibition of glial D-serine release rescues synaptic damage after brain injury. Glia. 2022 Feb 23. doi: 10.1002/glia.24161. Epub ahead of print. PMID: 35195906.

» View on PubMed

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Product Details

Name Ghost Dye™ Violet 540
Cat. No. 13-0879
Protocol Download Protocol
Excitation Laser Violet (405 nm)
Emission (nm) 537
Formulation 1 µL/test in DMSO
Application Flow Cytometry
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.