Ghost Dye™ Violet 510
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 13-0870-T100 | 100 tests | $44.00 | ||
| 13-0870-T500 | 500 tests (5 x 100 tests) | $170.00 |
Technical Data Sheet
Request Custom/BulkDescription
Ghost Dye™ Violet 510 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Violet 510 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.
Cindy L. Zuleger, Ph.D.
University of Wisconsin
Recent Publications:
Clark NM, Martinez LM, Murdock S, deLigio JT, Olex AL, Effi C, Dozmorov MG, Bos PD. Regulatory T Cells Support Breast Cancer Progression by Opposing IFN-γ-Dependent Functional Reprogramming of Myeloid Cells. Cell Rep. 2020 Dec 8;33(10):108482. doi: 10.1016/j.celrep.2020.108482. PMID: 33296659.
Li C, Park JH. Assessing IL-2-Induced STAT5 Phosphorylation in Fixed, Permeabilized Foxp3+ Treg Cells by Multiparameter Flow Cytometry. STAR Protoc. 2020 Dec 1;1(3):100195. doi: 10.1016/j.xpro.2020.100195. PMID: 33377089; PMCID: PMC7757559.
Heninger E, Sethakorn N, Kosoff D, Hematti P, Kuczler MD, Pienta KJ, Lang JM. Immune profiling of the bone marrow microenvironment in patients with high-risk localized prostate cancer. Oncotarget. 2020 Nov 17;11(46):4253-4265. doi: 10.18632/oncotarget.27817. PMID: 33245727; PMCID: PMC7679037.
Kline JM, Arriaga-Gomez E, Yangdon T, Boo B, et al. Repeated dermal application of the common preservative methylisothiazolinone triggers local inflammation, T cell influx, and prolonged mast cell-dependent tactile sensitivity in mice. PLoS One. 2020 Oct 26;15(10):e0241218. doi: 10.1371/journal.pone.0241218. PMID: 33104726.
Mishra S, Liao W, Liu Y, Yang M, Ma C, Wu H, Zhao M, Zhang X, Qiu Y, Lu Q, Zhang N. TGF-β and Eomes control the homeostasis of CD8+ regulatory T cells. J Exp Med. 2021 Jan 4;218(1):e20200030. doi: 10.1084/jem.20200030. PMID: 32991667.
Boyd DF, Allen EK, Randolph AG, Guo XJ, et al. Exuberant fibroblast activity compromises lung function via ADAMTS4. Nature. 2020 Oct 28. doi: 10.1038/s41586-020-2877-5. PMID: 33116313.
Clifton GT, Hale D, Vreeland TJ, et al. Results of a Randomized Phase IIb Trial of Nelipepimut-S + Trastuzumab versus Trastuzumab to Prevent Recurrences in Patients with High-Risk HER2 Low-Expressing Breast Cancer. Clin Cancer Res. 2020 Jun 1;26(11):2515-2523. doi: 10.1158/1078-0432.CCR-19-2741. Epub 2020 Feb 18. PMID: 32071118.
Wali S, Flores JR, Jaramillo AM, et al. Immune Modulation to Improve Survival of Viral Pneumonia in Mice. Am J Respir Cell Mol Biol. 2020;10.1165/rcmb.2020-0241OC. doi:10.1165/rcmb.2020-0241OC
Wu B, Chiang HC, Sun X, et al. Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation. J Immunother Cancer. 2020;8(2):e000964. doi:10.1136/jitc-2020-000964.
Shubina M, Tummers B, Boyd DF, et al. Necroptosis restricts influenza A virus as a stand-alone cell death mechanism. J Exp Med. 2020;217(11):e20191259. doi:10.1084/jem.20191259.
Kumar A, Suryadevara NC, Wolf KJ, et al. Heterotypic immunity against vaccinia virus in an HLA-B*07:02 transgenic mousepox infection model. Sci Rep. 2020;10(1):13167. Published 2020 Aug 5. doi:10.1038/s41598-020-69897-w.
Lustig A, Manor T, Shi G, et al. Lipid Microbubble-Conjugated Anti-CD3 and Anti-CD28 Antibodies (Microbubble-Based Human T Cell Activator) Offer Superior Long-Term Expansion of Human Naive T Cells In Vitro. Immunohorizons. 2020;4(8):475-484. Published 2020 Aug 7. doi:10.4049/immunohorizons.2000056.
Poholek CH, Raphael I, Wu D, et al. Noncanonical STAT3 activity sustains pathogenic Th17 proliferation and cytokine response to antigen. J Exp Med. 2020;217(10):e20191761. doi:10.1084/jem.20191761.
» View on Journal of Experimental Medicine
Jennifer S. Chen, Jessica D.S. Grassmann, Uthaman Gowthaman, Sam J. Olyha, Tregony Simoneau, M. Cecilia Berin, Stephanie C. Eisenbarth, Adam Williams. Flow cytometric identification of Tfh13 cells in mouse and human. Journal of Allergy and Clinical Immunology, 2020. https://doi.org/10.1016/j.jaci.2020.04.063.
Stearoyl-CoA desaturase mediated monounsaturated fatty acid availability supports humoral immunity. Xian Zhou, Xingxing Zhu, Chaofan Li, Yanfeng Li, Zhenqing Ye, Virginia ShapiroJohn A. Copland III, Taro Hitosugi, David Bernlohr, Jie Sun, Hu Zeng. bioRxiv 2020.04.22.028613; doi: https://doi.org/10.1101/2020.04.22.028613.
Hong-Xia ZhouRun-Feng LiYi-Feng Wang, et al. Total alkaloids from Alstonia scholaris inhibit influenza A virus replication and lung immunopathology by regulating the innate immune response. ScienceDirect. June 29 2020. https://doi.org/10.1016/j.phymed.2020.153272.
Marcovecchio, Paola M et al. Frontline Science: Kindlin-3 is essential for patrolling and phagocytosis functions of nonclassical monocytes during metastatic cancer surveillance. Journal of leukocyte biology vol. 107,6 (2020): 883-892. doi:10.1002/JLB.4HI0420-098R.
Mahuron KM, Moreau JM, Glasgow JE, et al. Layilin augments integrin activation to promote antitumor immunity. J Exp Med. 2020;217(9):e20192080. doi:10.1084/jem.20192080.
Logsdon, A.F., Schindler, A.G., Meabon, J.S. et al. Nitric oxide synthase mediates cerebellar dysfunction in mice exposed to repetitive blast-induced mild traumatic brain injury. Sci Rep 10, 9420 (2020). https://doi.org/10.1038/s41598-020-66113-7.
» View on Nature Communications
Irini Evnouchidou, Pascal Chappert, Samira Benadda, et al. IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses. Nat Commun. 2020 Jun 2;11(1):2779. doi: 10.1038/s41467-020-16471-7.
» View on Nature Communications
Nadia S. Kurd, Zhaoren He, Tiani L. Louis, et al. Chang. Early precursors and molecular determinants of tissue-resident memory CD8+ T lymphocytes revealed by single-cell RNA sequencing. Sci Immunol 2020 May 15;5(47):eaaz6894. doi: 10.1126/sciimmunol.aaz6894.
Caplan HW, Prabhakara KS, Kumar A, et al. Human cord blood-derived regulatory T-cell therapy modulates the central and peripheral immune response after traumatic brain injury. Stem Cells Transl Med. 2020 May 7:e12706. doi: 10.1002/sctm.19-0444.
Alexander J Neuwelt, Abigail K Kimball, Amber M Johnson, et al. Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms. J Immunother Cancer. 2020 Apr;8(1). pii: e000441. doi: 10.1136/jitc-2019-000441.
Alkhani A, Levy CS, Tsui M, et al. Ly6cLo non-classical monocytes promote resolution of rhesus rotavirus-mediated perinatal hepatic inflammation. Sci Rep. 2020 Apr 28;10(1):7165. doi: 10.1038/s41598-020-64158-2.
Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059
» View on PubMed
Sarika Saraswati, Stephanie M.W. Marrow, Lester A. Watch1 & Pampee P. Young. Identification of a pro-angiogenic functional role for FSP1-positive fibroblast subtype in wound healing. Nat Commun. 2019 Jul 9;10(1):3027. doi: 10.1038/s41467-019-10965-9
» View on PubMed
Martinov T, Swanson LA, Breed ER, Tucker CG, Dwyer AJ, Johnson JK, Mitchell JS, Sahli NL, Wilson JC, Singh LM, Hogquist KA, Spanier JA, Fife BT. Programmed Death-1 Restrains the Germinal Center in Type 1 Diabetes. J Immunol. 2019 Aug 15;203(4):844-852. doi: 10.4049/jimmunol.1801535
» View on PubMed
Kevin C. Osum, Adam L. Burrack, Tijana Martinov, et al. Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes. Sci Rep. 2018 May 29;8(1):8295. doi: 10.1038/s41598-018-26471-9
» View on PubMed
Avery L, Filderman J, Szymczak-Workman AL, Kane LP4. Tim-3 co-stimulation promotes short-lived effector T cells, restricts memory precursors, and is dispensable for T cell exhaustion. Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2455-2460. doi: 10.1073/pnas.1712107115
Mande P, Zirak B, Ko WC, Taravati K, Bride KL, Brodeur TY, Deng A, Dresser K, Jiang Z, Ettinger R, Fitzgerald KA, Rosenblum MD, Harris JE, Marshak-Rothstein A. Fas ligand promotes an inducible TLR-dependent model of cutaneous lupus-like inflammation. J Clin Invest. 2018 Jul 2;128(7):2966-2978. doi: 10.1172/JCI98219
» View on PubMed
Benck CJ, Martinov T, Fife BT and Chatterjea D. 2016. J. Vis. Exp. (107), e53638, doi:10.3791/53638. (Flow Cytometry)
Lee RA, Mao C, Vo H, Gao W and Zhong X. 2015. Ann NY Acad Sci. doi: 10.1111/nyas.12865. (Flow Cytometry) Watkin LB, Jessen B, Wiszniewski W, Vece TJ, Jan M, Sha Y, Thamsen M, Santos-Cortez RL et al. 2015. Nat Genet. 47: 654-660. (Flow Cytometry) Vo H, Chiu J, Allaimo D, Mao C, Wang Y, Gong Y, Ow H, Porter T and Zhong X. 2014. Immun, Inflamm & Dis. 2(4): 254-261. (Flow Cytometry) Miles B, Miller SM, Folkvord JM, Kimball A, Chamanian M, Meditz AL, Arends T et al. 2015. Nat Commun. doi: 10.1038/ncomms9608. (Flow Cytometry)Ghost Dye™ Violet 510 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Violet 510 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.
Cindy L. Zuleger, Ph.D.
University of Wisconsin
Recent Publications:
Clark NM, Martinez LM, Murdock S, deLigio JT, Olex AL, Effi C, Dozmorov MG, Bos PD. Regulatory T Cells Support Breast Cancer Progression by Opposing IFN-γ-Dependent Functional Reprogramming of Myeloid Cells. Cell Rep. 2020 Dec 8;33(10):108482. doi: 10.1016/j.celrep.2020.108482. PMID: 33296659.
Li C, Park JH. Assessing IL-2-Induced STAT5 Phosphorylation in Fixed, Permeabilized Foxp3+ Treg Cells by Multiparameter Flow Cytometry. STAR Protoc. 2020 Dec 1;1(3):100195. doi: 10.1016/j.xpro.2020.100195. PMID: 33377089; PMCID: PMC7757559.
Heninger E, Sethakorn N, Kosoff D, Hematti P, Kuczler MD, Pienta KJ, Lang JM. Immune profiling of the bone marrow microenvironment in patients with high-risk localized prostate cancer. Oncotarget. 2020 Nov 17;11(46):4253-4265. doi: 10.18632/oncotarget.27817. PMID: 33245727; PMCID: PMC7679037.
Kline JM, Arriaga-Gomez E, Yangdon T, Boo B, et al. Repeated dermal application of the common preservative methylisothiazolinone triggers local inflammation, T cell influx, and prolonged mast cell-dependent tactile sensitivity in mice. PLoS One. 2020 Oct 26;15(10):e0241218. doi: 10.1371/journal.pone.0241218. PMID: 33104726.
Mishra S, Liao W, Liu Y, Yang M, Ma C, Wu H, Zhao M, Zhang X, Qiu Y, Lu Q, Zhang N. TGF-β and Eomes control the homeostasis of CD8+ regulatory T cells. J Exp Med. 2021 Jan 4;218(1):e20200030. doi: 10.1084/jem.20200030. PMID: 32991667.
Boyd DF, Allen EK, Randolph AG, Guo XJ, et al. Exuberant fibroblast activity compromises lung function via ADAMTS4. Nature. 2020 Oct 28. doi: 10.1038/s41586-020-2877-5. PMID: 33116313.
Clifton GT, Hale D, Vreeland TJ, et al. Results of a Randomized Phase IIb Trial of Nelipepimut-S + Trastuzumab versus Trastuzumab to Prevent Recurrences in Patients with High-Risk HER2 Low-Expressing Breast Cancer. Clin Cancer Res. 2020 Jun 1;26(11):2515-2523. doi: 10.1158/1078-0432.CCR-19-2741. Epub 2020 Feb 18. PMID: 32071118.
Wali S, Flores JR, Jaramillo AM, et al. Immune Modulation to Improve Survival of Viral Pneumonia in Mice. Am J Respir Cell Mol Biol. 2020;10.1165/rcmb.2020-0241OC. doi:10.1165/rcmb.2020-0241OC
Wu B, Chiang HC, Sun X, et al. Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation. J Immunother Cancer. 2020;8(2):e000964. doi:10.1136/jitc-2020-000964.
Shubina M, Tummers B, Boyd DF, et al. Necroptosis restricts influenza A virus as a stand-alone cell death mechanism. J Exp Med. 2020;217(11):e20191259. doi:10.1084/jem.20191259.
Kumar A, Suryadevara NC, Wolf KJ, et al. Heterotypic immunity against vaccinia virus in an HLA-B*07:02 transgenic mousepox infection model. Sci Rep. 2020;10(1):13167. Published 2020 Aug 5. doi:10.1038/s41598-020-69897-w.
Lustig A, Manor T, Shi G, et al. Lipid Microbubble-Conjugated Anti-CD3 and Anti-CD28 Antibodies (Microbubble-Based Human T Cell Activator) Offer Superior Long-Term Expansion of Human Naive T Cells In Vitro. Immunohorizons. 2020;4(8):475-484. Published 2020 Aug 7. doi:10.4049/immunohorizons.2000056.
Poholek CH, Raphael I, Wu D, et al. Noncanonical STAT3 activity sustains pathogenic Th17 proliferation and cytokine response to antigen. J Exp Med. 2020;217(10):e20191761. doi:10.1084/jem.20191761.
» View on Journal of Experimental Medicine
Jennifer S. Chen, Jessica D.S. Grassmann, Uthaman Gowthaman, Sam J. Olyha, Tregony Simoneau, M. Cecilia Berin, Stephanie C. Eisenbarth, Adam Williams. Flow cytometric identification of Tfh13 cells in mouse and human. Journal of Allergy and Clinical Immunology, 2020. https://doi.org/10.1016/j.jaci.2020.04.063.
Stearoyl-CoA desaturase mediated monounsaturated fatty acid availability supports humoral immunity. Xian Zhou, Xingxing Zhu, Chaofan Li, Yanfeng Li, Zhenqing Ye, Virginia ShapiroJohn A. Copland III, Taro Hitosugi, David Bernlohr, Jie Sun, Hu Zeng. bioRxiv 2020.04.22.028613; doi: https://doi.org/10.1101/2020.04.22.028613.
Hong-Xia ZhouRun-Feng LiYi-Feng Wang, et al. Total alkaloids from Alstonia scholaris inhibit influenza A virus replication and lung immunopathology by regulating the innate immune response. ScienceDirect. June 29 2020. https://doi.org/10.1016/j.phymed.2020.153272.
Marcovecchio, Paola M et al. Frontline Science: Kindlin-3 is essential for patrolling and phagocytosis functions of nonclassical monocytes during metastatic cancer surveillance. Journal of leukocyte biology vol. 107,6 (2020): 883-892. doi:10.1002/JLB.4HI0420-098R.
Mahuron KM, Moreau JM, Glasgow JE, et al. Layilin augments integrin activation to promote antitumor immunity. J Exp Med. 2020;217(9):e20192080. doi:10.1084/jem.20192080.
Logsdon, A.F., Schindler, A.G., Meabon, J.S. et al. Nitric oxide synthase mediates cerebellar dysfunction in mice exposed to repetitive blast-induced mild traumatic brain injury. Sci Rep 10, 9420 (2020). https://doi.org/10.1038/s41598-020-66113-7.
» View on Nature Communications
Irini Evnouchidou, Pascal Chappert, Samira Benadda, et al. IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses. Nat Commun. 2020 Jun 2;11(1):2779. doi: 10.1038/s41467-020-16471-7.
» View on Nature Communications
Nadia S. Kurd, Zhaoren He, Tiani L. Louis, et al. Chang. Early precursors and molecular determinants of tissue-resident memory CD8+ T lymphocytes revealed by single-cell RNA sequencing. Sci Immunol 2020 May 15;5(47):eaaz6894. doi: 10.1126/sciimmunol.aaz6894.
Caplan HW, Prabhakara KS, Kumar A, et al. Human cord blood-derived regulatory T-cell therapy modulates the central and peripheral immune response after traumatic brain injury. Stem Cells Transl Med. 2020 May 7:e12706. doi: 10.1002/sctm.19-0444.
Alexander J Neuwelt, Abigail K Kimball, Amber M Johnson, et al. Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms. J Immunother Cancer. 2020 Apr;8(1). pii: e000441. doi: 10.1136/jitc-2019-000441.
Alkhani A, Levy CS, Tsui M, et al. Ly6cLo non-classical monocytes promote resolution of rhesus rotavirus-mediated perinatal hepatic inflammation. Sci Rep. 2020 Apr 28;10(1):7165. doi: 10.1038/s41598-020-64158-2.
Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059
» View on PubMed
Sarika Saraswati, Stephanie M.W. Marrow, Lester A. Watch1 & Pampee P. Young. Identification of a pro-angiogenic functional role for FSP1-positive fibroblast subtype in wound healing. Nat Commun. 2019 Jul 9;10(1):3027. doi: 10.1038/s41467-019-10965-9
» View on PubMed
Martinov T, Swanson LA, Breed ER, Tucker CG, Dwyer AJ, Johnson JK, Mitchell JS, Sahli NL, Wilson JC, Singh LM, Hogquist KA, Spanier JA, Fife BT. Programmed Death-1 Restrains the Germinal Center in Type 1 Diabetes. J Immunol. 2019 Aug 15;203(4):844-852. doi: 10.4049/jimmunol.1801535
» View on PubMed
Kevin C. Osum, Adam L. Burrack, Tijana Martinov, et al. Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes. Sci Rep. 2018 May 29;8(1):8295. doi: 10.1038/s41598-018-26471-9
» View on PubMed
Avery L, Filderman J, Szymczak-Workman AL, Kane LP4. Tim-3 co-stimulation promotes short-lived effector T cells, restricts memory precursors, and is dispensable for T cell exhaustion. Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2455-2460. doi: 10.1073/pnas.1712107115
Mande P, Zirak B, Ko WC, Taravati K, Bride KL, Brodeur TY, Deng A, Dresser K, Jiang Z, Ettinger R, Fitzgerald KA, Rosenblum MD, Harris JE, Marshak-Rothstein A. Fas ligand promotes an inducible TLR-dependent model of cutaneous lupus-like inflammation. J Clin Invest. 2018 Jul 2;128(7):2966-2978. doi: 10.1172/JCI98219
» View on PubMed
Benck CJ, Martinov T, Fife BT and Chatterjea D. 2016. J. Vis. Exp. (107), e53638, doi:10.3791/53638. (Flow Cytometry)
Lee RA, Mao C, Vo H, Gao W and Zhong X. 2015. Ann NY Acad Sci. doi: 10.1111/nyas.12865. (Flow Cytometry) Watkin LB, Jessen B, Wiszniewski W, Vece TJ, Jan M, Sha Y, Thamsen M, Santos-Cortez RL et al. 2015. Nat Genet. 47: 654-660. (Flow Cytometry) Vo H, Chiu J, Allaimo D, Mao C, Wang Y, Gong Y, Ow H, Porter T and Zhong X. 2014. Immun, Inflamm & Dis. 2(4): 254-261. (Flow Cytometry) Miles B, Miller SM, Folkvord JM, Kimball A, Chamanian M, Meditz AL, Arends T et al. 2015. Nat Commun. doi: 10.1038/ncomms9608. (Flow Cytometry)Product Details
| Name | Ghost Dye™ Violet 510 |
|---|---|
| Cat. No. | 13-0870 |
| Protocol | Download Protocol |
| Excitation Laser | Violet (405 nm) |
| Emission (nm) | 510 |
| Formulation | 1 µL/test in DMSO |
| Application | Flow Cytometry |
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.