Customer Service: (855) 848-6626 | 8:30 a.m - 4:30 p.m. PST | Happy New Year!

LEFT: Mouse thymocytes were incubated overnight at 4°C (dashed) or 37°C (solid) and stained with Ghost Violet 510.  RIGHT:  Mouse splenocytes were stimulated overnight with PMA and stained with Ghost Violet 510. Viable gate is indicated.
  • LEFT: Mouse thymocytes were incubated overnight at 4°C (dashed) or 37°C (solid) and stained with Ghost Violet 510.  RIGHT:  Mouse splenocytes were stimulated overnight with PMA and stained with Ghost Violet 510. Viable gate is indicated.
  • Ghost Dye™ Violet 510

Ghost Dye™ Violet 510

Cat No. Size Price Quantity
13-0870-T100 100 tests $44.00
13-0870-T500 500 tests (5 x 100 tests) $170.00

Description

Ghost Dye™ Violet 510 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Violet 510 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

I recently obtained an aliquot of Ghost Dye 510 from my core flow lab. I titrated the reagent against a mixture of heat-killed and untreated PBMC. Spooooky Bright! I will be replacing my current Live/Dead indicator with the Ghost Dye 510.

Cindy L. Zuleger, Ph.D.
University of Wisconsin

Recent Publications:

Wu D, Poholek CH, Majumder S, Liu Q, Revu SK, Mohib K, Rothstein DM, McGeachy MJ. IL-17-dependent fibroblastic reticular cell training boosts tissue protective mucosal immunity through IL-10-producing B cells. Sci Immunol. 2021 Dec 17;6(66):eaao3669. doi: 10.1126/sciimmunol.aao3669. Epub 2021 Dec 17. PMID: 34919443.

» View on PubMed

Wan Z, Sun R, Liu YW, Li S, Sun J, Li J, Zhu J, Moharil P, Zhang B, Ren P, Ren G, Zhang M, Ma X, Dai S, Yang D, Lu B, Li S. Targeting metabotropic glutamate receptor 4 for cancer immunotherapy. Sci Adv. 2021 Dec 10;7(50):eabj4226. doi: 10.1126/sciadv.abj4226. Epub 2021 Dec 10. PMID: 34890233. 

» View on PubMed

Wilfahrt D, Philips RL, Lama J, Kizerwetter M, Shapiro MJ, McCue SA, Kennedy MM, Rajcula MJ, Zeng H, Shapiro VS. Histone deacetylase 3 represses cholesterol efflux during CD4+ T-cell activation. Elife. 2021 Dec 2;10:e70978. doi: 10.7554/eLife.70978. PMID: 34854376; PMCID: PMC8639145. 

» View on PubMed

Nyhoff LE, Griffith AS, Clark ES, Thomas JW, Khan WN, Kendall PL. Btk Supports Autoreactive B Cell Development and Protects against Apoptosis but Is Expendable for Antigen Presentation. J Immunol. 2021 Nov 19:ji2000558. doi: 10.4049/jimmunol.2000558. Epub ahead of print. PMID: 34799428.

» View on Journal of Immunology

Young IC, Wu B, Andricovich J, Chuang ST, Li R, Tzatsos A, Wu RC, Wu MY. Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling. Cell Rep. 2021 Nov 23;37(8):110036. doi: 10.1016/j.celrep.2021.110036. PMID: 34818550.

» View on ScienceDirect

Prinzing B, Zebley CC, Petersen CT, Fan Y, Anido AA, Yi Z, Nguyen P, Houke H, Bell M, Haydar D, Brown C, Boi SK, Alli S, Crawford JC, Riberdy JM, Park JJ, Zhou S, Velasquez MP, DeRenzo C, Lazzarotto CR, Tsai SQ, Vogel P, Pruett-Miller SM, Langfitt DM, Gottschalk S, Youngblood B, Krenciute G. Deleting DNMT3A in CAR T cells prevents exhaustion and enhances antitumor activity. Sci Transl Med. 2021 Nov 17;13(620):eabh0272. doi: 10.1126/scitranslmed.abh0272. Epub 2021 Nov 17. PMID: 34788079.

» View on PubMed

Stephens WZ, Kubinak JL, Ghazaryan A, Bauer KM, Bell R, Buhrke K, Chiaro TR, Weis AM, Tang WW, Monts JK, Soto R, Ekiz HA, O'Connell RM, Round JL. Epithelial-myeloid exchange of MHC class II constrains immunity and microbiota composition. Cell Rep. 2021 Nov 2;37(5):109916. doi: 10.1016/j.celrep.2021.109916. PMID: 34731608.

» View on ScienceDirect

Boothby IC, Kinet MJ, Boda DP, Kwan EY, Clancy S, Cohen JN, Habrylo I, Lowe MM, Pauli M, Yates AE, Chan JD, Harris HW, Neuhaus IM, McCalmont TH, Molofsky AB, Rosenblum MD. Early-life inflammation primes a T helper 2 cell-fibroblast niche in skin. Nature. 2021 Oct 27. doi: 10.1038/s41586-021-04044-7. Epub ahead of print. PMID: 34707292.

 » View on Nature

Morgan PK, Huynh K, Pernes G, Miotto PM, Mellett NA, Giles C, Meikle PJ, Murphy AJ, Lancaster GI. Macrophage polarization state affects lipid composition and the channelling of exogenous fatty acids into endogenous lipid pools. J Biol Chem. 2021 Oct 22:101341. doi: 10.1016/j.jbc.2021.101341. PMID: 34695418.

 » View on ScienceDirect

Zebley CC, Abdelsamed HA, Ghoneim HE, Alli S, Brown C, Haydar D, Mi T, Harris T, McGargill MA, Krenciute G, Youngblood B. Proinflammatory cytokines promote TET2-mediated DNA demethylation during CD8 T cell effector differentiation. Cell Rep. 2021 Oct 12;37(2):109796. doi: 10.1016/j.celrep.2021.109796. PMID: 34644568.

 » View on ScienceDirect

Jin YB, Cao X, Shi CW, et al. Lactobacillus rhamnosus GG Promotes Early B Lineage Development and IgA Production in the Lamina Propria in Piglets. J Immunol. 2021 Sep 8: ji2100102. doi: 10.4049/jimmunol.2100102. PMID: 34497150.

» View on PubMed

Yagi M, Mizuno M, Fujisawa R, et al. Optimal Pore Size of Honeycomb Polylactic Acid Films for In Vitro Cartilage Formation by Synovial Mesenchymal Stem Cells. Stem Cells Int. 2021 Aug 2;2021:9239728. doi: 10.1155/2021/9239728. PMID: 34394358; PMCID: PMC8356005.

» View on PubMed

Lopez-Ichikawa M, Vu NK, Nijagal A, Rubinsky B, Chang TT. Neutrophils are important for the development of pro-reparative macrophages after irreversible electroporation of the liver in mice. Sci Rep. 2021 Jul 22;11(1):14986. doi: 10.1038/s41598-021-94016-8. PMID: 34294763.

» View on NatureCommunications

Dodagatta-Marri E, Ma HY, Liang B, et al. Integrin αvβ8 on T cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy. Cell Rep. 2021 Jul 6;36(1):109309. doi: 10.1016/j.celrep.2021.109309. PMID: 34233193.

» View on ScienceDirect

Xu C, Sun S, Johnson T, Qi R, Zhang S, Zhang J, Yang K. The glutathione peroxidase Gpx4 prevents lipid peroxidation and ferroptosis to sustain Treg cell activation and suppression of antitumor immunity. Cell Rep. 2021 Jun 15;35(11):109235. doi: 10.1016/j.celrep.2021.109235. PMID: 34133924.

» View on ScienceDirect

Yan C, Saleh N, Yang J, et al. Novel induction of CD40 expression by tumor cells with RAS/RAF/PI3K pathway inhibition augments response to checkpoint blockade. Mol Cancer. 2021 Jun 6;20(1):85. doi: 10.1186/s12943-021-01366-y. PMID: 34092233; PMCID: PMC8182921.

» View on PubMed

Dikiy S, Li J, Bai L, Jiang M, Janke L, Zong X, Hao X, Hoyos B, Wang ZM, Xu B, Fan Y, Rudensky AY, Feng Y. A distal Foxp3 enhancer enables interleukin-2 dependent thymic Treg cell lineage commitment for robust immune tolerance. Immunity. 2021 May 11;54(5):931-946.e11. doi: 10.1016/j.immuni.2021.03.020. Epub 2021 Apr 9. PMID: 33838102; PMCID: PMC8317508.

 » View on PubMed

Battaglia NG, Uccello TP, Hughson A, et al. Co-administration of a clinically relevant dexamethasone dosage with ablative radiotherapy reduces peripheral lymphocytes but does not alter in vivo intratumoral lymphocyte phenotype or inhibit efficacy of radiotherapy in a murine colorectal tumor model. Int J Radiat Oncol Biol Phys. 2021 Apr 29:S0360-3016(21)00403-X. doi: 10.1016/j.ijrobp.2021.04.021. PMID: 33933481.

» View on ScienceDirect

Clark NM, Martinez LM, Murdock S, deLigio JT, Olex AL, Effi C, Dozmorov MG, Bos PD. Regulatory T Cells Support Breast Cancer Progression by Opposing IFN-γ-Dependent Functional Reprogramming of Myeloid Cells. Cell Rep. 2020 Dec 8;33(10):108482. doi: 10.1016/j.celrep.2020.108482. PMID: 33296659.

» View on ScienceDirect

Li C, Park JH. Assessing IL-2-Induced STAT5 Phosphorylation in Fixed, Permeabilized Foxp3+ Treg Cells by Multiparameter Flow Cytometry. STAR Protoc. 2020 Dec 1;1(3):100195. doi: 10.1016/j.xpro.2020.100195. PMID: 33377089; PMCID: PMC7757559.

» View on PubMed

Heninger E, Sethakorn N, Kosoff D, Hematti P, Kuczler MD, Pienta KJ, Lang JM. Immune profiling of the bone marrow microenvironment in patients with high-risk localized prostate cancer. Oncotarget. 2020 Nov 17;11(46):4253-4265. doi: 10.18632/oncotarget.27817. PMID: 33245727; PMCID: PMC7679037.

» View on PubMed

Kline JM, Arriaga-Gomez E, Yangdon T, Boo B, et al. Repeated dermal application of the common preservative methylisothiazolinone triggers local inflammation, T cell influx, and prolonged mast cell-dependent tactile sensitivity in mice. PLoS One. 2020 Oct 26;15(10):e0241218. doi: 10.1371/journal.pone.0241218. PMID: 33104726.

» View on PubMed

Mishra S, Liao W, Liu Y, Yang M, Ma C, Wu H, Zhao M, Zhang X, Qiu Y, Lu Q, Zhang N. TGF-β and Eomes control the homeostasis of CD8+ regulatory T cells. J Exp Med. 2021 Jan 4;218(1):e20200030. doi: 10.1084/jem.20200030. PMID: 32991667.  

» View on PubMed

Boyd DF, Allen EK, Randolph AG, Guo XJ, et al. Exuberant fibroblast activity compromises lung function via ADAMTS4. Nature. 2020 Oct 28. doi: 10.1038/s41586-020-2877-5. PMID: 33116313.

» View on Nature

Clifton GT, Hale D, Vreeland TJ, et al. Results of a Randomized Phase IIb Trial of Nelipepimut-S + Trastuzumab versus Trastuzumab to Prevent Recurrences in Patients with High-Risk HER2 Low-Expressing Breast Cancer. Clin Cancer Res. 2020 Jun 1;26(11):2515-2523. doi: 10.1158/1078-0432.CCR-19-2741. Epub 2020 Feb 18. PMID: 32071118.

» View on PubMed

Wali S, Flores JR, Jaramillo AM, et al. Immune Modulation to Improve Survival of Viral Pneumonia in Mice.  Am J Respir Cell Mol Biol. 2020;10.1165/rcmb.2020-0241OC. doi:10.1165/rcmb.2020-0241OC

» View on PubMed

Wu B, Chiang HC, Sun X, et al. Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation. J Immunother Cancer. 2020;8(2):e000964. doi:10.1136/jitc-2020-000964.

» View on PubMed

Shubina M, Tummers B, Boyd DF, et al. Necroptosis restricts influenza A virus as a stand-alone cell death mechanism. J Exp Med. 2020;217(11):e20191259. doi:10.1084/jem.20191259.

» View on PubMed

Kumar A, Suryadevara NC, Wolf KJ, et al. Heterotypic immunity against vaccinia virus in an HLA-B*07:02 transgenic mousepox infection model. Sci Rep. 2020;10(1):13167. Published 2020 Aug 5. doi:10.1038/s41598-020-69897-w.

» View on Nature

Lustig A, Manor T, Shi G, et al. Lipid Microbubble-Conjugated Anti-CD3 and Anti-CD28 Antibodies (Microbubble-Based Human T Cell Activator) Offer Superior Long-Term Expansion of Human Naive T Cells In Vitro. Immunohorizons. 2020;4(8):475-484. Published 2020 Aug 7. doi:10.4049/immunohorizons.2000056.

» View on PubMed

Poholek CH, Raphael I, Wu D, et al. Noncanonical STAT3 activity sustains pathogenic Th17 proliferation and cytokine response to antigen. J Exp Med. 2020;217(10):e20191761. doi:10.1084/jem.20191761.

» View on Journal of Experimental Medicine

Jennifer S. Chen, Jessica D.S. Grassmann, Uthaman Gowthaman, Sam J. Olyha, Tregony Simoneau, M. Cecilia Berin, Stephanie C. Eisenbarth, Adam Williams. Flow cytometric identification of Tfh13 cells in mouse and human. Journal of Allergy and Clinical Immunology, 2020.  https://doi.org/10.1016/j.jaci.2020.04.063.

» View on ScienceDirect

Stearoyl-CoA desaturase mediated monounsaturated fatty acid availability supports humoral immunity. Xian Zhou, Xingxing Zhu, Chaofan Li, Yanfeng Li, Zhenqing Ye, Virginia ShapiroJohn A. Copland III, Taro Hitosugi, David Bernlohr, Jie Sun, Hu Zeng. bioRxiv 2020.04.22.028613; doi: https://doi.org/10.1101/2020.04.22.028613.

 » View on bioRxiv 

Hong-Xia ZhouRun-Feng LiYi-Feng Wang, et al. Total alkaloids from Alstonia scholaris inhibit influenza A virus replication and lung immunopathology by regulating the innate immune response.  ScienceDirect.  June 29 2020. https://doi.org/10.1016/j.phymed.2020.153272.

 » View on ScienceDirect

Marcovecchio, Paola M et al. Frontline Science: Kindlin-3 is essential for patrolling and phagocytosis functions of nonclassical monocytes during metastatic cancer surveillance. Journal of leukocyte biology vol. 107,6 (2020): 883-892. doi:10.1002/JLB.4HI0420-098R.

 » View on PubMed

Mahuron KM, Moreau JM, Glasgow JE, et al. Layilin augments integrin activation to promote antitumor immunity. J Exp Med. 2020;217(9):e20192080. doi:10.1084/jem.20192080.

 » View on PubMed

Logsdon, A.F., Schindler, A.G., Meabon, J.S. et al. Nitric oxide synthase mediates cerebellar dysfunction in mice exposed to repetitive blast-induced mild traumatic brain injury. Sci Rep 10, 9420 (2020). https://doi.org/10.1038/s41598-020-66113-7.

 » View on Nature Communications

Irini EvnouchidouPascal Chappert, Samira Benadda, et al. IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses. Nat Commun. 2020 Jun 2;11(1):2779. doi: 10.1038/s41467-020-16471-7.

» View on Nature Communications

Nadia S. Kurd, Zhaoren He, Tiani L. Louis, et al. Chang. Early precursors and molecular determinants of tissue-resident memory CD8+ T lymphocytes revealed by single-cell RNA sequencing. Sci Immunol 2020 May 15;5(47):eaaz6894. doi: 10.1126/sciimmunol.aaz6894.

» View on ScienceImmunology

Caplan HW, Prabhakara KS, Kumar A, et al. Human cord blood-derived regulatory T-cell therapy modulates the central and peripheral immune response after traumatic brain injury. Stem Cells Transl Med. 2020 May 7:e12706. doi: 10.1002/sctm.19-0444.

» View on PubMed

Alexander J Neuwelt, Abigail K Kimball, Amber M Johnson, et al. Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms.  J Immunother Cancer. 2020 Apr;8(1). pii: e000441. doi: 10.1136/jitc-2019-000441.

» View on PubMed

Alkhani A, Levy CS, Tsui M, et al. Ly6cLo non-classical monocytes promote resolution of rhesus rotavirus-mediated perinatal hepatic inflammation. Sci Rep. 2020 Apr 28;10(1):7165. doi: 10.1038/s41598-020-64158-2.

» View on PubMed

Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059

» View on PubMed

Sarika Saraswati, Stephanie M.W. Marrow, Lester A. Watch1 & Pampee P. Young. Identification of a pro-angiogenic functional role for FSP1-positive fibroblast subtype in wound healing. Nat Commun. 2019 Jul 9;10(1):3027. doi: 10.1038/s41467-019-10965-9

» View on PubMed

Martinov T, Swanson LA, Breed ER, Tucker CG, Dwyer AJ, Johnson JK, Mitchell JS, Sahli NL, Wilson JC, Singh LM, Hogquist KA, Spanier JA, Fife BT. Programmed Death-1 Restrains the Germinal Center in Type 1 Diabetes. J Immunol. 2019 Aug 15;203(4):844-852. doi: 10.4049/jimmunol.1801535

» View on PubMed

Kevin C. Osum, Adam L. Burrack, Tijana Martinov, et al. Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes. Sci Rep. 2018 May 29;8(1):8295. doi: 10.1038/s41598-018-26471-9

» View on PubMed

Avery L, Filderman J, Szymczak-Workman AL, Kane LP4. Tim-3 co-stimulation promotes short-lived effector T cells, restricts memory precursors, and is dispensable for T cell exhaustion. Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2455-2460. doi: 10.1073/pnas.1712107115

» View on PubMed

Mande P, Zirak B, Ko WC, Taravati K, Bride KL, Brodeur TY, Deng A, Dresser K, Jiang Z, Ettinger R, Fitzgerald KA, Rosenblum MD, Harris JE, Marshak-Rothstein A. Fas ligand promotes an inducible TLR-dependent model of cutaneous lupus-like inflammation. J Clin Invest. 2018 Jul 2;128(7):2966-2978. doi: 10.1172/JCI98219

» View on PubMed

Benck CJ, Martinov T, Fife BT and Chatterjea D. 2016. J. Vis. Exp. (107), e53638, doi:10.3791/53638. (Flow Cytometry)

Lee RA, Mao C, Vo H, Gao W and Zhong X. 2015. Ann NY Acad Sci. doi: 10.1111/nyas.12865. (Flow Cytometry) Watkin LB, Jessen B, Wiszniewski W, Vece TJ, Jan M, Sha Y, Thamsen M, Santos-Cortez RL et al. 2015. Nat Genet. 47: 654-660. (Flow Cytometry) Vo H, Chiu J, Allaimo D, Mao C, Wang Y, Gong Y, Ow H, Porter T and Zhong X. 2014. Immun, Inflamm & Dis. 2(4): 254-261. (Flow Cytometry) Miles B, Miller SM, Folkvord JM, Kimball A, Chamanian M, Meditz AL, Arends T et al. 2015. Nat Commun. doi: 10.1038/ncomms9608. (Flow Cytometry)
View More

Ghost Dye™ Violet 510 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Violet 510 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

I recently obtained an aliquot of Ghost Dye 510 from my core flow lab. I titrated the reagent against a mixture of heat-killed and untreated PBMC. Spooooky Bright! I will be replacing my current Live/Dead indicator with the Ghost Dye 510.

Cindy L. Zuleger, Ph.D.
University of Wisconsin

Recent Publications:

Wu D, Poholek CH, Majumder S, Liu Q, Revu SK, Mohib K, Rothstein DM, McGeachy MJ. IL-17-dependent fibroblastic reticular cell training boosts tissue protective mucosal immunity through IL-10-producing B cells. Sci Immunol. 2021 Dec 17;6(66):eaao3669. doi: 10.1126/sciimmunol.aao3669. Epub 2021 Dec 17. PMID: 34919443.

» View on PubMed

Wan Z, Sun R, Liu YW, Li S, Sun J, Li J, Zhu J, Moharil P, Zhang B, Ren P, Ren G, Zhang M, Ma X, Dai S, Yang D, Lu B, Li S. Targeting metabotropic glutamate receptor 4 for cancer immunotherapy. Sci Adv. 2021 Dec 10;7(50):eabj4226. doi: 10.1126/sciadv.abj4226. Epub 2021 Dec 10. PMID: 34890233. 

» View on PubMed

Wilfahrt D, Philips RL, Lama J, Kizerwetter M, Shapiro MJ, McCue SA, Kennedy MM, Rajcula MJ, Zeng H, Shapiro VS. Histone deacetylase 3 represses cholesterol efflux during CD4+ T-cell activation. Elife. 2021 Dec 2;10:e70978. doi: 10.7554/eLife.70978. PMID: 34854376; PMCID: PMC8639145. 

» View on PubMed

Nyhoff LE, Griffith AS, Clark ES, Thomas JW, Khan WN, Kendall PL. Btk Supports Autoreactive B Cell Development and Protects against Apoptosis but Is Expendable for Antigen Presentation. J Immunol. 2021 Nov 19:ji2000558. doi: 10.4049/jimmunol.2000558. Epub ahead of print. PMID: 34799428.

» View on Journal of Immunology

Young IC, Wu B, Andricovich J, Chuang ST, Li R, Tzatsos A, Wu RC, Wu MY. Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling. Cell Rep. 2021 Nov 23;37(8):110036. doi: 10.1016/j.celrep.2021.110036. PMID: 34818550.

» View on ScienceDirect

Prinzing B, Zebley CC, Petersen CT, Fan Y, Anido AA, Yi Z, Nguyen P, Houke H, Bell M, Haydar D, Brown C, Boi SK, Alli S, Crawford JC, Riberdy JM, Park JJ, Zhou S, Velasquez MP, DeRenzo C, Lazzarotto CR, Tsai SQ, Vogel P, Pruett-Miller SM, Langfitt DM, Gottschalk S, Youngblood B, Krenciute G. Deleting DNMT3A in CAR T cells prevents exhaustion and enhances antitumor activity. Sci Transl Med. 2021 Nov 17;13(620):eabh0272. doi: 10.1126/scitranslmed.abh0272. Epub 2021 Nov 17. PMID: 34788079.

» View on PubMed

Stephens WZ, Kubinak JL, Ghazaryan A, Bauer KM, Bell R, Buhrke K, Chiaro TR, Weis AM, Tang WW, Monts JK, Soto R, Ekiz HA, O'Connell RM, Round JL. Epithelial-myeloid exchange of MHC class II constrains immunity and microbiota composition. Cell Rep. 2021 Nov 2;37(5):109916. doi: 10.1016/j.celrep.2021.109916. PMID: 34731608.

» View on ScienceDirect

Boothby IC, Kinet MJ, Boda DP, Kwan EY, Clancy S, Cohen JN, Habrylo I, Lowe MM, Pauli M, Yates AE, Chan JD, Harris HW, Neuhaus IM, McCalmont TH, Molofsky AB, Rosenblum MD. Early-life inflammation primes a T helper 2 cell-fibroblast niche in skin. Nature. 2021 Oct 27. doi: 10.1038/s41586-021-04044-7. Epub ahead of print. PMID: 34707292.

 » View on Nature

Morgan PK, Huynh K, Pernes G, Miotto PM, Mellett NA, Giles C, Meikle PJ, Murphy AJ, Lancaster GI. Macrophage polarization state affects lipid composition and the channelling of exogenous fatty acids into endogenous lipid pools. J Biol Chem. 2021 Oct 22:101341. doi: 10.1016/j.jbc.2021.101341. PMID: 34695418.

 » View on ScienceDirect

Zebley CC, Abdelsamed HA, Ghoneim HE, Alli S, Brown C, Haydar D, Mi T, Harris T, McGargill MA, Krenciute G, Youngblood B. Proinflammatory cytokines promote TET2-mediated DNA demethylation during CD8 T cell effector differentiation. Cell Rep. 2021 Oct 12;37(2):109796. doi: 10.1016/j.celrep.2021.109796. PMID: 34644568.

 » View on ScienceDirect

Jin YB, Cao X, Shi CW, et al. Lactobacillus rhamnosus GG Promotes Early B Lineage Development and IgA Production in the Lamina Propria in Piglets. J Immunol. 2021 Sep 8: ji2100102. doi: 10.4049/jimmunol.2100102. PMID: 34497150.

» View on PubMed

Yagi M, Mizuno M, Fujisawa R, et al. Optimal Pore Size of Honeycomb Polylactic Acid Films for In Vitro Cartilage Formation by Synovial Mesenchymal Stem Cells. Stem Cells Int. 2021 Aug 2;2021:9239728. doi: 10.1155/2021/9239728. PMID: 34394358; PMCID: PMC8356005.

» View on PubMed

Lopez-Ichikawa M, Vu NK, Nijagal A, Rubinsky B, Chang TT. Neutrophils are important for the development of pro-reparative macrophages after irreversible electroporation of the liver in mice. Sci Rep. 2021 Jul 22;11(1):14986. doi: 10.1038/s41598-021-94016-8. PMID: 34294763.

» View on NatureCommunications

Dodagatta-Marri E, Ma HY, Liang B, et al. Integrin αvβ8 on T cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy. Cell Rep. 2021 Jul 6;36(1):109309. doi: 10.1016/j.celrep.2021.109309. PMID: 34233193.

» View on ScienceDirect

Xu C, Sun S, Johnson T, Qi R, Zhang S, Zhang J, Yang K. The glutathione peroxidase Gpx4 prevents lipid peroxidation and ferroptosis to sustain Treg cell activation and suppression of antitumor immunity. Cell Rep. 2021 Jun 15;35(11):109235. doi: 10.1016/j.celrep.2021.109235. PMID: 34133924.

» View on ScienceDirect

Yan C, Saleh N, Yang J, et al. Novel induction of CD40 expression by tumor cells with RAS/RAF/PI3K pathway inhibition augments response to checkpoint blockade. Mol Cancer. 2021 Jun 6;20(1):85. doi: 10.1186/s12943-021-01366-y. PMID: 34092233; PMCID: PMC8182921.

» View on PubMed

Dikiy S, Li J, Bai L, Jiang M, Janke L, Zong X, Hao X, Hoyos B, Wang ZM, Xu B, Fan Y, Rudensky AY, Feng Y. A distal Foxp3 enhancer enables interleukin-2 dependent thymic Treg cell lineage commitment for robust immune tolerance. Immunity. 2021 May 11;54(5):931-946.e11. doi: 10.1016/j.immuni.2021.03.020. Epub 2021 Apr 9. PMID: 33838102; PMCID: PMC8317508.

 » View on PubMed

Battaglia NG, Uccello TP, Hughson A, et al. Co-administration of a clinically relevant dexamethasone dosage with ablative radiotherapy reduces peripheral lymphocytes but does not alter in vivo intratumoral lymphocyte phenotype or inhibit efficacy of radiotherapy in a murine colorectal tumor model. Int J Radiat Oncol Biol Phys. 2021 Apr 29:S0360-3016(21)00403-X. doi: 10.1016/j.ijrobp.2021.04.021. PMID: 33933481.

» View on ScienceDirect

Clark NM, Martinez LM, Murdock S, deLigio JT, Olex AL, Effi C, Dozmorov MG, Bos PD. Regulatory T Cells Support Breast Cancer Progression by Opposing IFN-γ-Dependent Functional Reprogramming of Myeloid Cells. Cell Rep. 2020 Dec 8;33(10):108482. doi: 10.1016/j.celrep.2020.108482. PMID: 33296659.

» View on ScienceDirect

Li C, Park JH. Assessing IL-2-Induced STAT5 Phosphorylation in Fixed, Permeabilized Foxp3+ Treg Cells by Multiparameter Flow Cytometry. STAR Protoc. 2020 Dec 1;1(3):100195. doi: 10.1016/j.xpro.2020.100195. PMID: 33377089; PMCID: PMC7757559.

» View on PubMed

Heninger E, Sethakorn N, Kosoff D, Hematti P, Kuczler MD, Pienta KJ, Lang JM. Immune profiling of the bone marrow microenvironment in patients with high-risk localized prostate cancer. Oncotarget. 2020 Nov 17;11(46):4253-4265. doi: 10.18632/oncotarget.27817. PMID: 33245727; PMCID: PMC7679037.

» View on PubMed

Kline JM, Arriaga-Gomez E, Yangdon T, Boo B, et al. Repeated dermal application of the common preservative methylisothiazolinone triggers local inflammation, T cell influx, and prolonged mast cell-dependent tactile sensitivity in mice. PLoS One. 2020 Oct 26;15(10):e0241218. doi: 10.1371/journal.pone.0241218. PMID: 33104726.

» View on PubMed

Mishra S, Liao W, Liu Y, Yang M, Ma C, Wu H, Zhao M, Zhang X, Qiu Y, Lu Q, Zhang N. TGF-β and Eomes control the homeostasis of CD8+ regulatory T cells. J Exp Med. 2021 Jan 4;218(1):e20200030. doi: 10.1084/jem.20200030. PMID: 32991667.  

» View on PubMed

Boyd DF, Allen EK, Randolph AG, Guo XJ, et al. Exuberant fibroblast activity compromises lung function via ADAMTS4. Nature. 2020 Oct 28. doi: 10.1038/s41586-020-2877-5. PMID: 33116313.

» View on Nature

Clifton GT, Hale D, Vreeland TJ, et al. Results of a Randomized Phase IIb Trial of Nelipepimut-S + Trastuzumab versus Trastuzumab to Prevent Recurrences in Patients with High-Risk HER2 Low-Expressing Breast Cancer. Clin Cancer Res. 2020 Jun 1;26(11):2515-2523. doi: 10.1158/1078-0432.CCR-19-2741. Epub 2020 Feb 18. PMID: 32071118.

» View on PubMed

Wali S, Flores JR, Jaramillo AM, et al. Immune Modulation to Improve Survival of Viral Pneumonia in Mice.  Am J Respir Cell Mol Biol. 2020;10.1165/rcmb.2020-0241OC. doi:10.1165/rcmb.2020-0241OC

» View on PubMed

Wu B, Chiang HC, Sun X, et al. Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation. J Immunother Cancer. 2020;8(2):e000964. doi:10.1136/jitc-2020-000964.

» View on PubMed

Shubina M, Tummers B, Boyd DF, et al. Necroptosis restricts influenza A virus as a stand-alone cell death mechanism. J Exp Med. 2020;217(11):e20191259. doi:10.1084/jem.20191259.

» View on PubMed

Kumar A, Suryadevara NC, Wolf KJ, et al. Heterotypic immunity against vaccinia virus in an HLA-B*07:02 transgenic mousepox infection model. Sci Rep. 2020;10(1):13167. Published 2020 Aug 5. doi:10.1038/s41598-020-69897-w.

» View on Nature

Lustig A, Manor T, Shi G, et al. Lipid Microbubble-Conjugated Anti-CD3 and Anti-CD28 Antibodies (Microbubble-Based Human T Cell Activator) Offer Superior Long-Term Expansion of Human Naive T Cells In Vitro. Immunohorizons. 2020;4(8):475-484. Published 2020 Aug 7. doi:10.4049/immunohorizons.2000056.

» View on PubMed

Poholek CH, Raphael I, Wu D, et al. Noncanonical STAT3 activity sustains pathogenic Th17 proliferation and cytokine response to antigen. J Exp Med. 2020;217(10):e20191761. doi:10.1084/jem.20191761.

» View on Journal of Experimental Medicine

Jennifer S. Chen, Jessica D.S. Grassmann, Uthaman Gowthaman, Sam J. Olyha, Tregony Simoneau, M. Cecilia Berin, Stephanie C. Eisenbarth, Adam Williams. Flow cytometric identification of Tfh13 cells in mouse and human. Journal of Allergy and Clinical Immunology, 2020.  https://doi.org/10.1016/j.jaci.2020.04.063.

» View on ScienceDirect

Stearoyl-CoA desaturase mediated monounsaturated fatty acid availability supports humoral immunity. Xian Zhou, Xingxing Zhu, Chaofan Li, Yanfeng Li, Zhenqing Ye, Virginia ShapiroJohn A. Copland III, Taro Hitosugi, David Bernlohr, Jie Sun, Hu Zeng. bioRxiv 2020.04.22.028613; doi: https://doi.org/10.1101/2020.04.22.028613.

 » View on bioRxiv 

Hong-Xia ZhouRun-Feng LiYi-Feng Wang, et al. Total alkaloids from Alstonia scholaris inhibit influenza A virus replication and lung immunopathology by regulating the innate immune response.  ScienceDirect.  June 29 2020. https://doi.org/10.1016/j.phymed.2020.153272.

 » View on ScienceDirect

Marcovecchio, Paola M et al. Frontline Science: Kindlin-3 is essential for patrolling and phagocytosis functions of nonclassical monocytes during metastatic cancer surveillance. Journal of leukocyte biology vol. 107,6 (2020): 883-892. doi:10.1002/JLB.4HI0420-098R.

 » View on PubMed

Mahuron KM, Moreau JM, Glasgow JE, et al. Layilin augments integrin activation to promote antitumor immunity. J Exp Med. 2020;217(9):e20192080. doi:10.1084/jem.20192080.

 » View on PubMed

Logsdon, A.F., Schindler, A.G., Meabon, J.S. et al. Nitric oxide synthase mediates cerebellar dysfunction in mice exposed to repetitive blast-induced mild traumatic brain injury. Sci Rep 10, 9420 (2020). https://doi.org/10.1038/s41598-020-66113-7.

 » View on Nature Communications

Irini EvnouchidouPascal Chappert, Samira Benadda, et al. IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses. Nat Commun. 2020 Jun 2;11(1):2779. doi: 10.1038/s41467-020-16471-7.

» View on Nature Communications

Nadia S. Kurd, Zhaoren He, Tiani L. Louis, et al. Chang. Early precursors and molecular determinants of tissue-resident memory CD8+ T lymphocytes revealed by single-cell RNA sequencing. Sci Immunol 2020 May 15;5(47):eaaz6894. doi: 10.1126/sciimmunol.aaz6894.

» View on ScienceImmunology

Caplan HW, Prabhakara KS, Kumar A, et al. Human cord blood-derived regulatory T-cell therapy modulates the central and peripheral immune response after traumatic brain injury. Stem Cells Transl Med. 2020 May 7:e12706. doi: 10.1002/sctm.19-0444.

» View on PubMed

Alexander J Neuwelt, Abigail K Kimball, Amber M Johnson, et al. Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms.  J Immunother Cancer. 2020 Apr;8(1). pii: e000441. doi: 10.1136/jitc-2019-000441.

» View on PubMed

Alkhani A, Levy CS, Tsui M, et al. Ly6cLo non-classical monocytes promote resolution of rhesus rotavirus-mediated perinatal hepatic inflammation. Sci Rep. 2020 Apr 28;10(1):7165. doi: 10.1038/s41598-020-64158-2.

» View on PubMed

Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059

» View on PubMed

Sarika Saraswati, Stephanie M.W. Marrow, Lester A. Watch1 & Pampee P. Young. Identification of a pro-angiogenic functional role for FSP1-positive fibroblast subtype in wound healing. Nat Commun. 2019 Jul 9;10(1):3027. doi: 10.1038/s41467-019-10965-9

» View on PubMed

Martinov T, Swanson LA, Breed ER, Tucker CG, Dwyer AJ, Johnson JK, Mitchell JS, Sahli NL, Wilson JC, Singh LM, Hogquist KA, Spanier JA, Fife BT. Programmed Death-1 Restrains the Germinal Center in Type 1 Diabetes. J Immunol. 2019 Aug 15;203(4):844-852. doi: 10.4049/jimmunol.1801535

» View on PubMed

Kevin C. Osum, Adam L. Burrack, Tijana Martinov, et al. Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes. Sci Rep. 2018 May 29;8(1):8295. doi: 10.1038/s41598-018-26471-9

» View on PubMed

Avery L, Filderman J, Szymczak-Workman AL, Kane LP4. Tim-3 co-stimulation promotes short-lived effector T cells, restricts memory precursors, and is dispensable for T cell exhaustion. Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2455-2460. doi: 10.1073/pnas.1712107115

» View on PubMed

Mande P, Zirak B, Ko WC, Taravati K, Bride KL, Brodeur TY, Deng A, Dresser K, Jiang Z, Ettinger R, Fitzgerald KA, Rosenblum MD, Harris JE, Marshak-Rothstein A. Fas ligand promotes an inducible TLR-dependent model of cutaneous lupus-like inflammation. J Clin Invest. 2018 Jul 2;128(7):2966-2978. doi: 10.1172/JCI98219

» View on PubMed

Benck CJ, Martinov T, Fife BT and Chatterjea D. 2016. J. Vis. Exp. (107), e53638, doi:10.3791/53638. (Flow Cytometry)

Lee RA, Mao C, Vo H, Gao W and Zhong X. 2015. Ann NY Acad Sci. doi: 10.1111/nyas.12865. (Flow Cytometry) Watkin LB, Jessen B, Wiszniewski W, Vece TJ, Jan M, Sha Y, Thamsen M, Santos-Cortez RL et al. 2015. Nat Genet. 47: 654-660. (Flow Cytometry) Vo H, Chiu J, Allaimo D, Mao C, Wang Y, Gong Y, Ow H, Porter T and Zhong X. 2014. Immun, Inflamm & Dis. 2(4): 254-261. (Flow Cytometry) Miles B, Miller SM, Folkvord JM, Kimball A, Chamanian M, Meditz AL, Arends T et al. 2015. Nat Commun. doi: 10.1038/ncomms9608. (Flow Cytometry)
Hide

Product Details

Name Ghost Dye™ Violet 510
Cat. No. 13-0870
Protocol Download Protocol
Excitation Laser Violet (405 nm)
Emission (nm) 510
Formulation 1 µL/test in DMSO
Application Flow Cytometry
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.