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LEFT: Mouse thymocytes were incubated overnight at 4°C (dashed) or 37°C (solid) and stained with Ghost Violet 450.  RIGHT:  Mouse splenocytes were stimulated overnight with PMA and stained with Ghost Violet 450. Viable gate is indicated.
  • LEFT: Mouse thymocytes were incubated overnight at 4°C (dashed) or 37°C (solid) and stained with Ghost Violet 450.  RIGHT:  Mouse splenocytes were stimulated overnight with PMA and stained with Ghost Violet 450. Viable gate is indicated.
  • Ghost Dye™ Violet 450

Ghost Dye™ Violet 450

Cat No. Size Price Quantity
13-0863-T100 100 tests $44.00
13-0863-T500 500 tests (5 x 100 tests) $170.00

Description

Ghost Dye™ Violet 450 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Violet 450 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

 

Recent Publications:

Poholek CH, Raphael I, Wu D, et al. Noncanonical STAT3 activity sustains pathogenic Th17 proliferation and cytokine response to antigen. J Exp Med. 2020;217(10):e20191761. doi:10.1084/jem.20191761.

» View on Journal of Experimental Medicine

Zhou W, Zhang J, Toki S, et al. COX Inhibition Increases Alternaria-Induced Pulmonary Group 2 Innate Lymphoid Cell Responses and IL-33 Release in Mice [published online ahead of print, 2020 Jul 20]. J Immunol. 2020;ji1901544. doi:10.4049/jimmunol.1901544.

» View on Journal of Immunology

Terranova-Barberio, M., Pawlowska, N., Dhawan, M. et al. Exhausted T cell signature predicts immunotherapy response in ER-positive breast cancer. Nat Commun 11, 3584 (2020). https://doi.org/10.1038/s41467-020-17414-y.

» View on Nature

Alexander J Neuwelt, Abigail K Kimball, Amber M Johnson, Benjamin W Arnold, Bonnie L Bullock, Rachael E Kaspar, Emily K Kleczko, Jeff W Kwak, Meng-Han Wu, Lynn E Heasley, Robert C Doebele, Howard Y Li,Raphael A Nemenoff, Eric T Clambey. Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms.  J Immunother Cancer. 2020 Apr;8(1). pii: e000441. doi: 10.1136/jitc-2019-000441.

» View on PubMed

Miles B, Miller SM, Folkvord JM, Kimball A, Chamanian M, Meditz AL, Arends T et al. 2015. Nat Commun. doi: 10.1038/ncomms9608. (Flow Cytometry)

View More
Ghost Dye™ Violet 450 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Violet 450 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

 

Recent Publications:

Poholek CH, Raphael I, Wu D, et al. Noncanonical STAT3 activity sustains pathogenic Th17 proliferation and cytokine response to antigen. J Exp Med. 2020;217(10):e20191761. doi:10.1084/jem.20191761.

» View on Journal of Experimental Medicine

Zhou W, Zhang J, Toki S, et al. COX Inhibition Increases Alternaria-Induced Pulmonary Group 2 Innate Lymphoid Cell Responses and IL-33 Release in Mice [published online ahead of print, 2020 Jul 20]. J Immunol. 2020;ji1901544. doi:10.4049/jimmunol.1901544.

» View on Journal of Immunology

Terranova-Barberio, M., Pawlowska, N., Dhawan, M. et al. Exhausted T cell signature predicts immunotherapy response in ER-positive breast cancer. Nat Commun 11, 3584 (2020). https://doi.org/10.1038/s41467-020-17414-y.

» View on Nature

Alexander J Neuwelt, Abigail K Kimball, Amber M Johnson, Benjamin W Arnold, Bonnie L Bullock, Rachael E Kaspar, Emily K Kleczko, Jeff W Kwak, Meng-Han Wu, Lynn E Heasley, Robert C Doebele, Howard Y Li,Raphael A Nemenoff, Eric T Clambey. Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms.  J Immunother Cancer. 2020 Apr;8(1). pii: e000441. doi: 10.1136/jitc-2019-000441.

» View on PubMed

Miles B, Miller SM, Folkvord JM, Kimball A, Chamanian M, Meditz AL, Arends T et al. 2015. Nat Commun. doi: 10.1038/ncomms9608. (Flow Cytometry)

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Product Details

Name Ghost Dye™ Violet 450
Cat. No. 13-0863
Protocol Download Protocol
Excitation Laser Violet (405 nm)
Emission (nm) 450
Formulation 1 µL/test in DMSO
Application Flow Cytometry
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.