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Mouse splenocytes were stimulated overnight with PMA and ionomycin and stained with Ghost UV 450. Viable gate is indicated.
  • Mouse splenocytes were stimulated overnight with PMA and ionomycin and stained with Ghost UV 450. Viable gate is indicated.
  • Ghost Dye™ UV 450

Ghost Dye™ UV 450

Cat No. Size Price Quantity
13-0868-T100 100 tests $44.00
13-0868-T500 500 tests (5 x 100 tests) $170.00

Description

Ghost Dye™ UV 450 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ UV 450 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

 

Recent Publications:

 

Marcia de Almeida Monteiro Melo Ferraz, Mayako Fujihara, Jennifer Beth NagashimaMichael James NoonanMiho Inoue-MurayamaNucharin Songsasen. Follicular Extracellular Vesicles Enhance Meiotic Resumption of Domestic Cat Vitrified Oocytes. Sci Rep2020 May 25;10(1):8619. doi: 10.1038/s41598-020-65497-w.

» View on PubMed

Derek W. Trobaugh, Chengqun Sun, Matthew D. Dunn, Douglas S. Reed, William B. Klimstra. Rational design of a live-attenuated eastern equine encephalitis virus vaccine through informed mutation of virulence determinants. PLoS Pathog. 2019 Feb 11;15(2):e1007584. doi: 10.1371/journal.ppat.1007584
» View on PubMed

Jessica K. Fiege, Ian A. Stone, Elizabeth J. Fay, Matthew W. Markman, Sathi Wijeyesinghe, Marissa G. Macchietto, Steven Shen, David Masopust and Ryan A. Langlois. The Impact of TCR Signal Strength on Resident Memory T Cell Formation during Influenza Virus Infection. J Immunol. 2019 Aug 15;203(4):936-945. doi: 10.4049/jimmunol.1900093
» View on PubMed

Jessica K. Fiege, Ian A. Stone, Rebekah E. Dumm, Barbara M. Waring, Brian T. FifeI, Judith Agudo, Brian D. Brown, Nicholas S. Heaton, Ryan A. Langlois. Long-term surviving influenza infected cells evade CD8+ T cell mediated clearance. PLoS Pathog. 2019 Sep 26;15(9):e1008077. doi: 10.1371/journal.ppat.1008077
» View on PubMed

 

Name Ghost Dye™ UV 450
Cat. No. 13-0868
Protocol Download Protocol
Excitation Laser UV (355 nm)
Emission (nm) 450
Formulation 1 µL/test in DMSO
Application Flow Cytometry
View More

Ghost Dye™ UV 450 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ UV 450 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

 

Recent Publications:

 

Marcia de Almeida Monteiro Melo Ferraz, Mayako Fujihara, Jennifer Beth NagashimaMichael James NoonanMiho Inoue-MurayamaNucharin Songsasen. Follicular Extracellular Vesicles Enhance Meiotic Resumption of Domestic Cat Vitrified Oocytes. Sci Rep2020 May 25;10(1):8619. doi: 10.1038/s41598-020-65497-w.

» View on PubMed

Derek W. Trobaugh, Chengqun Sun, Matthew D. Dunn, Douglas S. Reed, William B. Klimstra. Rational design of a live-attenuated eastern equine encephalitis virus vaccine through informed mutation of virulence determinants. PLoS Pathog. 2019 Feb 11;15(2):e1007584. doi: 10.1371/journal.ppat.1007584
» View on PubMed

Jessica K. Fiege, Ian A. Stone, Elizabeth J. Fay, Matthew W. Markman, Sathi Wijeyesinghe, Marissa G. Macchietto, Steven Shen, David Masopust and Ryan A. Langlois. The Impact of TCR Signal Strength on Resident Memory T Cell Formation during Influenza Virus Infection. J Immunol. 2019 Aug 15;203(4):936-945. doi: 10.4049/jimmunol.1900093
» View on PubMed

Jessica K. Fiege, Ian A. Stone, Rebekah E. Dumm, Barbara M. Waring, Brian T. FifeI, Judith Agudo, Brian D. Brown, Nicholas S. Heaton, Ryan A. Langlois. Long-term surviving influenza infected cells evade CD8+ T cell mediated clearance. PLoS Pathog. 2019 Sep 26;15(9):e1008077. doi: 10.1371/journal.ppat.1008077
» View on PubMed

 

Name Ghost Dye™ UV 450
Cat. No. 13-0868
Protocol Download Protocol
Excitation Laser UV (355 nm)
Emission (nm) 450
Formulation 1 µL/test in DMSO
Application Flow Cytometry
Hide

Product Details

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.