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LEFT: Mouse thymocytes were incubated overnight at 4°C (dashed) or 37°C (solid) and stained with Ghost Red 780.   RIGHT:  Mouse splenocytes were stimulated overnight with PMA and stained with Ghost Red 780. Viable gate is indicated.
  • LEFT: Mouse thymocytes were incubated overnight at 4°C (dashed) or 37°C (solid) and stained with Ghost Red 780.   RIGHT:  Mouse splenocytes were stimulated overnight with PMA and stained with Ghost Red 780. Viable gate is indicated.
  • Ghost Dye™ Red 780

Ghost Dye™ Red 780

Cat No. Size Price Quantity
13-0865-T100 100 tests $44.00
13-0865-T500 500 tests (5 x 100 tests) $170.00

Description

Ghost Dye™ Red 780 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Red 780 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

 

Recent Publications: 

Davenport BJ, Morrison TE, Kedl RM, Klarquist J. Conserved and Novel Mouse CD8 T Cell Epitopes within SARS-CoV-2 Spike Receptor Binding Domain Protein Identified following Subunit Vaccination. J Immunol. 2021 May 10:ji2100195. doi: 10.4049/jimmunol.2100195. PMID: 33972373.

» View on JournalofImmunology

Marín-Jiménez JA, Capasso A, Lewis MS, et al. Testing Cancer Immunotherapy in a Human Immune System Mouse Model: Correlating Treatment Responses to Human Chimerism, Therapeutic Variables and Immune Cell Phenotypes. Front Immunol. 2021 Mar 29;12:607282. doi: 10.3389/fimmu.2021.607282. PMID: 33854497; PMCID: PMC8040953.

» View on PubMed

Lee W, Kingstad-Bakke B, Kedl RM, Kawaoka Y, Suresh M. CCR2 Regulates Vaccine-Induced Mucosal T-Cell Memory to Influenza A Virus. bioRxiv. 2021 Mar 25:2021.03.24.436901. doi: 10.1101/2021.03.24.436901. PMID: 33791695; PMCID: PMC8010722.

» View on PubMed

Mitchell JE, Lund MM, Starmer J, Ge K, Magnuson T, Shpargel KB, Whitmire JK. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021 Apr 13;35(2):108966. doi: 10.1016/j.celrep.2021.108966. PMID: 33852868.

» View on ScienceDirect

Reimann M, Schrezenmeier JF, Richter-Pechanska P, Dolnik A, et al. Adaptive T-cell immunity controls senescence-prone MyD88- or CARD11-mutant B-cell lymphomas. Blood. 2020 Nov 24:blood.2020005244. doi: 10.1182/blood.2020005244. PMID: 33232972.

» View on PubMed

McCoy SS, Giri J, Das R, et al. Minor salivary gland mesenchymal stromal cells derived from patients with Sjӧgren's syndrome deploy intact immune plasticity. Cytotherapy. 2020 Nov 28:S1465-3249(20)30861-6. doi: 10.1016/j.jcyt.2020.09.008. PMID: 33262072.

» View on PubMed

Fisher C, Patel R. Rifampin, Rifapentine, and Rifabutin are Active Against Intracellular Periprosthetic Joint Infection-Associated Staphylococcus epidermidis. Antimicrob Agents Chemother. 2020 Nov 16:AAC.01275-20. doi: 10.1128/AAC.01275-20. PMID: 33199387.

» View on PubMed

Bescucci DM, Clarke ST, Brown CLJ, et al. The absence of murine cathelicidin-related antimicrobial peptide impacts host responses enhancing Salmonella enterica serovar Typhimurium infection. Gut Pathog. 2020 Nov 13;12(1):53. doi: 10.1186/s13099-020-00386-1. PMID: 33292444; PMCID: PMC7666523.

» View on PubMed

Schmidt JK, Keding LT, Block LN, et al. Placenta-derived macaque trophoblast stem cells: differentiation to syncytiotrophoblasts and extravillous trophoblasts reveals phenotypic reprogramming. Sci Rep. 2020 Nov 5;10(1):19159. doi: 10.1038/s41598-020-76313-w. PMID: 33154556; PMCID: PMC7644694.

» View on Scientific Reports

Kong H, Reczek CR, McElroy GS, et al. Metabolic determinants of cellular fitness dependent on mitochondrial reactive oxygen species. Sci Adv. 2020 Nov 4;6(45):eabb7272. doi: 10.1126/sciadv.abb7272. PMID: 33148642.

» View on PubMed

View More

Ghost Dye™ Red 780 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Red 780 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

 

Recent Publications: 

Davenport BJ, Morrison TE, Kedl RM, Klarquist J. Conserved and Novel Mouse CD8 T Cell Epitopes within SARS-CoV-2 Spike Receptor Binding Domain Protein Identified following Subunit Vaccination. J Immunol. 2021 May 10:ji2100195. doi: 10.4049/jimmunol.2100195. PMID: 33972373.

» View on JournalofImmunology

Marín-Jiménez JA, Capasso A, Lewis MS, et al. Testing Cancer Immunotherapy in a Human Immune System Mouse Model: Correlating Treatment Responses to Human Chimerism, Therapeutic Variables and Immune Cell Phenotypes. Front Immunol. 2021 Mar 29;12:607282. doi: 10.3389/fimmu.2021.607282. PMID: 33854497; PMCID: PMC8040953.

» View on PubMed

Lee W, Kingstad-Bakke B, Kedl RM, Kawaoka Y, Suresh M. CCR2 Regulates Vaccine-Induced Mucosal T-Cell Memory to Influenza A Virus. bioRxiv. 2021 Mar 25:2021.03.24.436901. doi: 10.1101/2021.03.24.436901. PMID: 33791695; PMCID: PMC8010722.

» View on PubMed

Mitchell JE, Lund MM, Starmer J, Ge K, Magnuson T, Shpargel KB, Whitmire JK. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021 Apr 13;35(2):108966. doi: 10.1016/j.celrep.2021.108966. PMID: 33852868.

» View on ScienceDirect

Reimann M, Schrezenmeier JF, Richter-Pechanska P, Dolnik A, et al. Adaptive T-cell immunity controls senescence-prone MyD88- or CARD11-mutant B-cell lymphomas. Blood. 2020 Nov 24:blood.2020005244. doi: 10.1182/blood.2020005244. PMID: 33232972.

» View on PubMed

McCoy SS, Giri J, Das R, et al. Minor salivary gland mesenchymal stromal cells derived from patients with Sjӧgren's syndrome deploy intact immune plasticity. Cytotherapy. 2020 Nov 28:S1465-3249(20)30861-6. doi: 10.1016/j.jcyt.2020.09.008. PMID: 33262072.

» View on PubMed

Fisher C, Patel R. Rifampin, Rifapentine, and Rifabutin are Active Against Intracellular Periprosthetic Joint Infection-Associated Staphylococcus epidermidis. Antimicrob Agents Chemother. 2020 Nov 16:AAC.01275-20. doi: 10.1128/AAC.01275-20. PMID: 33199387.

» View on PubMed

Bescucci DM, Clarke ST, Brown CLJ, et al. The absence of murine cathelicidin-related antimicrobial peptide impacts host responses enhancing Salmonella enterica serovar Typhimurium infection. Gut Pathog. 2020 Nov 13;12(1):53. doi: 10.1186/s13099-020-00386-1. PMID: 33292444; PMCID: PMC7666523.

» View on PubMed

Schmidt JK, Keding LT, Block LN, et al. Placenta-derived macaque trophoblast stem cells: differentiation to syncytiotrophoblasts and extravillous trophoblasts reveals phenotypic reprogramming. Sci Rep. 2020 Nov 5;10(1):19159. doi: 10.1038/s41598-020-76313-w. PMID: 33154556; PMCID: PMC7644694.

» View on Scientific Reports

Kong H, Reczek CR, McElroy GS, et al. Metabolic determinants of cellular fitness dependent on mitochondrial reactive oxygen species. Sci Adv. 2020 Nov 4;6(45):eabb7272. doi: 10.1126/sciadv.abb7272. PMID: 33148642.

» View on PubMed

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Product Details

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.