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LEFT: Mouse thymocytes were incubated overnight at 4°C (dashed) or 37°C (solid) and stained with Ghost Red 780.   RIGHT:  Mouse splenocytes were stimulated overnight with PMA and stained with Ghost Red 780. Viable gate is indicated.
  • LEFT: Mouse thymocytes were incubated overnight at 4°C (dashed) or 37°C (solid) and stained with Ghost Red 780.   RIGHT:  Mouse splenocytes were stimulated overnight with PMA and stained with Ghost Red 780. Viable gate is indicated.
  • Ghost Dye™ Red 780

Ghost Dye™ Red 780

Cat No. Size Price Quantity
13-0865-T100 100 tests $44.00
13-0865-T500 500 tests (5 x 100 tests) $170.00

Description

Ghost Dye™ Red 780 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Red 780 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

Recent Publications:

Andrea PennatiEmily A Nylen, Ian D Duncan, Jacques Galipeau . Regulatory B Cells Normalize CNS Myeloid Cell Content in a Mouse Model of Multiple Sclerosis and Promote Oligodendrogenesis and Remyelination. J Neuroscience. 2020 May 19;JN-RM-2840-19. doi: 10.1523/JNEUROSCI.2840-19.2020. 

» View on PubMed

Wormsbaecher C, Hindman AR, Avendano A, et al. In utero estrogenic endocrine disruption alters the stroma to increase extracellular matrix density and mammary gland stiffness. Breast Cancer Res. 2020;22(1):41. Published 2020 May 5. doi:10.1186/s13058-020-01275-w.

» View on PubMed

Giri J, Galipeau J. Mesenchymal stromal cell therapeutic potency is dependent upon viability, route of delivery, and immune match. Blood Adv. 2020 May 12;4(9):1987-1997. doi: 10.1182/bloodadvances.2020001711.

» View on PubMed

Roth TL, Li PJ, Blaeschke F, Nies JF, Apathy R, Mowery C, Yu R, Nguyen MLT, Lee Y, Truong A, Hiatt J, Wu D, Nguyen DN, Goodman D, Bluestone JA, Ye CJ, Roybal K, Shifrut E, Marson A. Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies. Cell. 2020 Apr 15. pii: S0092-8674(20)30332-9. doi: 10.1016/j.cell.2020.03.039.

» View on ScienceDirect

Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059

» View on PubMed

Jessica K. Fiege, Ian A. Stone, Elizabeth J. Fay, Matthew W. Markman, Sathi Wijeyesinghe, Marissa G. Macchietto, Steven Shen, David Masopust and Ryan A. Langlois. The Impact of TCR Signal Strength on Resident Memory T Cell Formation during Influenza Virus Infection. J Immunol. 2019 Aug 15;203(4):936-945. doi: 10.4049/jimmunol.1900093

» View on PubMed

Jessica K. Fiege, Ian A. Stone, Rebekah E. Dumm, Barbara M. Waring, Brian T. FifeI, Judith Agudo, Brian D. Brown, Nicholas S. Heaton, Ryan A. Langlois. Long-term surviving influenza infected cells evade CD8+ T cell mediated clearance. PLoS Pathog. 2019 Sep 26;15(9):e1008077. doi: 10.1371/journal.ppat.1008077

» View on PubMed

Matthew A. Huggins, Frances V. Sjaastad, Mark Pierson, Tamara A. Kucaba, Whitney Swanson, Christopher Staley, Alexa R. Weingarden, Isaac J. Jensen, Derek B. Danahy, Vladimir P. Badovinac, Stephen C. Jameson, Vaiva Vezys, David Masopust, Alexander Khoruts, Thomas S. Griffith, and Sara E. Hamilton. Microbial Exposure Enhances Immunity to Pathogens Recognized by TLR2 but Increases Susceptibility to Cytokine Storm through TLR4 Sensitization. Cell Rep. 2019 Aug 13;28(7):1729-1743.e5. doi: 10.1016/j.celrep.2019.07.028

» View on PubMed

Uzodinma U. Uche, Ann R. Piccirillo, Shunsuke Kataoka, Stephanie J. Grebinoski, Louise M. D’Cruz, and Lawrence P. Kane. PIK3IP1/TrIP restricts activation of T cells through inhibition of PI3K/Akt. J Exp Med. 2018 Dec 3;215(12):3165-3179. doi: 10.1084/jem.20172018

» View on PubMed

Manan M. Mehta, Samuel E. Weinberg, Elizabeth M. Steinert, Krishan Chhiba, Carlos Alberto Martinez, Peng Gao, Harris R. Perlman, Paul Bryce, Nissim Hay, and Navdeep S. Chandel. Hexokinase 2 is dispensable for T cell-dependent immunity. Cancer Metab. 2018 Aug 17;6:10. doi: 10.1186/s40170-018-0184-5

» View on PubMed

Pushpak Bhattacharjee & Peter A. Keyel. Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses. Sci Rep. 2018 Apr 4;8(1):6458. doi: 10.1038/s41598-018-24955-2

» View on PubMed

Guthmiller JJ, Zander RA and Butler NS. 2015. Methods Mol Biol. 1325: 19-37. (Flow Cytometry)

Malhotra D, Linehan JL, Dileepan T, Lee YJ, Purtha WE, Lu JV, Nelson RW, Fife BT, Orr HT, Anderson MS, Hogquist KA and Jenkins MK. 2016. Nat Immunol. doi: 10.1038/ni.3327. (Flow Cytometry)

View More
Ghost Dye™ Red 780 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Red 780 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

Recent Publications:

Andrea PennatiEmily A Nylen, Ian D Duncan, Jacques Galipeau . Regulatory B Cells Normalize CNS Myeloid Cell Content in a Mouse Model of Multiple Sclerosis and Promote Oligodendrogenesis and Remyelination. J Neuroscience. 2020 May 19;JN-RM-2840-19. doi: 10.1523/JNEUROSCI.2840-19.2020. 

» View on PubMed

Wormsbaecher C, Hindman AR, Avendano A, et al. In utero estrogenic endocrine disruption alters the stroma to increase extracellular matrix density and mammary gland stiffness. Breast Cancer Res. 2020;22(1):41. Published 2020 May 5. doi:10.1186/s13058-020-01275-w.

» View on PubMed

Giri J, Galipeau J. Mesenchymal stromal cell therapeutic potency is dependent upon viability, route of delivery, and immune match. Blood Adv. 2020 May 12;4(9):1987-1997. doi: 10.1182/bloodadvances.2020001711.

» View on PubMed

Roth TL, Li PJ, Blaeschke F, Nies JF, Apathy R, Mowery C, Yu R, Nguyen MLT, Lee Y, Truong A, Hiatt J, Wu D, Nguyen DN, Goodman D, Bluestone JA, Ye CJ, Roybal K, Shifrut E, Marson A. Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies. Cell. 2020 Apr 15. pii: S0092-8674(20)30332-9. doi: 10.1016/j.cell.2020.03.039.

» View on ScienceDirect

Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059

» View on PubMed

Jessica K. Fiege, Ian A. Stone, Elizabeth J. Fay, Matthew W. Markman, Sathi Wijeyesinghe, Marissa G. Macchietto, Steven Shen, David Masopust and Ryan A. Langlois. The Impact of TCR Signal Strength on Resident Memory T Cell Formation during Influenza Virus Infection. J Immunol. 2019 Aug 15;203(4):936-945. doi: 10.4049/jimmunol.1900093

» View on PubMed

Jessica K. Fiege, Ian A. Stone, Rebekah E. Dumm, Barbara M. Waring, Brian T. FifeI, Judith Agudo, Brian D. Brown, Nicholas S. Heaton, Ryan A. Langlois. Long-term surviving influenza infected cells evade CD8+ T cell mediated clearance. PLoS Pathog. 2019 Sep 26;15(9):e1008077. doi: 10.1371/journal.ppat.1008077

» View on PubMed

Matthew A. Huggins, Frances V. Sjaastad, Mark Pierson, Tamara A. Kucaba, Whitney Swanson, Christopher Staley, Alexa R. Weingarden, Isaac J. Jensen, Derek B. Danahy, Vladimir P. Badovinac, Stephen C. Jameson, Vaiva Vezys, David Masopust, Alexander Khoruts, Thomas S. Griffith, and Sara E. Hamilton. Microbial Exposure Enhances Immunity to Pathogens Recognized by TLR2 but Increases Susceptibility to Cytokine Storm through TLR4 Sensitization. Cell Rep. 2019 Aug 13;28(7):1729-1743.e5. doi: 10.1016/j.celrep.2019.07.028

» View on PubMed

Uzodinma U. Uche, Ann R. Piccirillo, Shunsuke Kataoka, Stephanie J. Grebinoski, Louise M. D’Cruz, and Lawrence P. Kane. PIK3IP1/TrIP restricts activation of T cells through inhibition of PI3K/Akt. J Exp Med. 2018 Dec 3;215(12):3165-3179. doi: 10.1084/jem.20172018

» View on PubMed

Manan M. Mehta, Samuel E. Weinberg, Elizabeth M. Steinert, Krishan Chhiba, Carlos Alberto Martinez, Peng Gao, Harris R. Perlman, Paul Bryce, Nissim Hay, and Navdeep S. Chandel. Hexokinase 2 is dispensable for T cell-dependent immunity. Cancer Metab. 2018 Aug 17;6:10. doi: 10.1186/s40170-018-0184-5

» View on PubMed

Pushpak Bhattacharjee & Peter A. Keyel. Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses. Sci Rep. 2018 Apr 4;8(1):6458. doi: 10.1038/s41598-018-24955-2

» View on PubMed

Guthmiller JJ, Zander RA and Butler NS. 2015. Methods Mol Biol. 1325: 19-37. (Flow Cytometry)

Malhotra D, Linehan JL, Dileepan T, Lee YJ, Purtha WE, Lu JV, Nelson RW, Fife BT, Orr HT, Anderson MS, Hogquist KA and Jenkins MK. 2016. Nat Immunol. doi: 10.1038/ni.3327. (Flow Cytometry)

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Product Details

Name Ghost Dye™ Red 780
Cat. No. 13-0865
Protocol Download Protocol
Excitation Laser Red (633 nm)
Emission (nm) 780
Formulation 1 µL/test in DMSO
Application Flow Cytometry
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.