LEFT: Mouse thymocytes were incubated overnight at 4°C (dashed) or 37°C (solid) and stained with Ghost Red 780.   RIGHT:  Mouse splenocytes were stimulated overnight with PMA and stained with Ghost Red 780. Viable gate is indicated.
  • LEFT: Mouse thymocytes were incubated overnight at 4°C (dashed) or 37°C (solid) and stained with Ghost Red 780.   RIGHT:  Mouse splenocytes were stimulated overnight with PMA and stained with Ghost Red 780. Viable gate is indicated.
  • Ghost Dye™ Red 780

Ghost Dye™ Red 780

Cat No. Size Price Quantity
13-0865-T100 100 tests $44.00
13-0865-T500 500 tests (5 x 100 tests) $170.00

Description

Ghost Dye™ Red 780 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Red 780 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

 

Recent Publications: 

Stephens WZ, Kubinak JL, Ghazaryan A, Bauer KM, Bell R, Buhrke K, Chiaro TR, Weis AM, Tang WW, Monts JK, Soto R, Ekiz HA, O'Connell RM, Round JL. Epithelial-myeloid exchange of MHC class II constrains immunity and microbiota composition. Cell Rep. 2021 Nov 2;37(5):109916. doi: 10.1016/j.celrep.2021.109916. PMID: 34731608.

» View on ScienceDirect

Fiege JK, Block KE, Pierson MJ, Nanda H, Shepherd FK, Mickelson CK, Stolley JM, Matchett WE, Wijeyesinghe S, Meyerholz DK, Vezys V, Shen SS, Hamilton SE, Masopust D, Langlois RA. Mice with diverse microbial exposure histories as a model for preclinical vaccine testing. Cell Host Microbe. 2021 Oct 27:S1931-3128(21)00463-7. doi: 10.1016/j.chom.2021.10.001. Epub ahead of print. PMID: 34731647.

 » View on ScienceDirect

Sturmlechner I, Zhang C, Sine CC, van Deursen EJ, Jeganathan KB, Hamada N, Grasic J, Friedman D, Stutchman JT, Can I, Hamada M, Lim DY, Lee JH, Ordog T, Laberge RM, Shapiro V, Baker DJ, Li H, van Deursen JM. p21 produces a bioactive secretome that places stressed cells under immunosurveillance. Science. 2021 Oct 29;374(6567):eabb3420. doi: 10.1126/science.abb3420. Epub 2021 Oct 29. PMID: 34709885.

 » View on Science

Ayupe AC, Beckedorff F, Levay K, Yon B, Salgueiro Y, Shiekhattar R, Park KK. Identification of long noncoding RNAs in injury-resilient and injury-susceptible mouse retinal ganglion cells. BMC Genomics. 2021 Oct 14;22(1):741. doi: 10.1186/s12864-021-08050-x. PMID: 34649511.

» View on PubMed

Tei R, Iijima K, Matsumoto K, et al. TLR3-driven IFN-β antagonizes STAT5-activating cytokines and suppresses innate type 2 response in the lung. J Allergy Clin Immunol. 2021 Aug 21:S0091-6749(21)01296-3. doi: 10.1016/j.jaci.2021.07.041. PMID: 34428519.

» View on ScienceDirect

Magee K, Marsh IR, Turek MM, et al. Safety and feasibility of an in situ vaccination and immunomodulatory targeted radionuclide combination immuno-radiotherapy approach in a comparative (companion dog) setting. PLoS One. 2021 Aug 12;16(8):e0255798. doi: 10.1371/journal.pone.0255798. PMID: 34383787; PMCID: PMC8360580.

» View on PubMed

Jairaman A, Othy S, Dynes JL, et al. Piezo1 channels restrain regulatory T cells but are dispensable for effector CD4+ T cell responses. Sci Adv. 2021 Jul 7;7(28):eabg5859. doi: 10.1126/sciadv.abg5859. PMID: 34233878.

» View on PubMed

Burbach BJ, O'Flanagan SD, Shao Q, et al. Irreversible electroporation augments checkpoint immunotherapy in prostate cancer and promotes tumor antigen-specific tissue-resident memory CD8+ T cells. Nat Commun. 2021 Jun 23;12(1):3862. doi: 10.1038/s41467-021-24132-6. PMID: 34162858.

» View on NatureCommunications

Hamilton JR, Tsuchida CA, Nguyen DN, et al. Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering. Cell Rep. 2021 Jun 1;35(9):109207. doi: 10.1016/j.celrep.2021.109207. PMID: 34077734.

 » View on ScienceDirect

Davenport BJ, Morrison TE, Kedl RM, Klarquist J. Conserved and Novel Mouse CD8 T Cell Epitopes within SARS-CoV-2 Spike Receptor Binding Domain Protein Identified following Subunit Vaccination. J Immunol. 2021 May 10:ji2100195. doi: 10.4049/jimmunol.2100195. PMID: 33972373.

» View on JournalofImmunology

Trott DW, Islam MT, Buckley DJ, Donato AJ, Dutson T, Sorensen ES, Cai J, Gogulamudi VR, Phuong TTT, Lesniewski LA. T lymphocyte depletion ameliorates age-related metabolic impairments in mice. Geroscience. 2021 Apr 24. doi: 10.1007/s11357-021-00368-4. PMID: 33893902.

» View on PubMed

Marín-Jiménez JA, Capasso A, Lewis MS, et al. Testing Cancer Immunotherapy in a Human Immune System Mouse Model: Correlating Treatment Responses to Human Chimerism, Therapeutic Variables and Immune Cell Phenotypes. Front Immunol. 2021 Mar 29;12:607282. doi: 10.3389/fimmu.2021.607282. PMID: 33854497; PMCID: PMC8040953.

» View on PubMed

Lee W, Kingstad-Bakke B, Kedl RM, Kawaoka Y, Suresh M. CCR2 Regulates Vaccine-Induced Mucosal T-Cell Memory to Influenza A Virus. bioRxiv. 2021 Mar 25:2021.03.24.436901. doi: 10.1101/2021.03.24.436901. PMID: 33791695; PMCID: PMC8010722.

» View on PubMed

Mitchell JE, Lund MM, Starmer J, Ge K, Magnuson T, Shpargel KB, Whitmire JK. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021 Apr 13;35(2):108966. doi: 10.1016/j.celrep.2021.108966. PMID: 33852868.

» View on ScienceDirect

Reimann M, Schrezenmeier JF, Richter-Pechanska P, Dolnik A, et al. Adaptive T-cell immunity controls senescence-prone MyD88- or CARD11-mutant B-cell lymphomas. Blood. 2020 Nov 24:blood.2020005244. doi: 10.1182/blood.2020005244. PMID: 33232972.

» View on PubMed

McCoy SS, Giri J, Das R, et al. Minor salivary gland mesenchymal stromal cells derived from patients with Sjӧgren's syndrome deploy intact immune plasticity. Cytotherapy. 2020 Nov 28:S1465-3249(20)30861-6. doi: 10.1016/j.jcyt.2020.09.008. PMID: 33262072.

» View on PubMed

Fisher C, Patel R. Rifampin, Rifapentine, and Rifabutin are Active Against Intracellular Periprosthetic Joint Infection-Associated Staphylococcus epidermidis. Antimicrob Agents Chemother. 2020 Nov 16:AAC.01275-20. doi: 10.1128/AAC.01275-20. PMID: 33199387.

» View on PubMed

Bescucci DM, Clarke ST, Brown CLJ, et al. The absence of murine cathelicidin-related antimicrobial peptide impacts host responses enhancing Salmonella enterica serovar Typhimurium infection. Gut Pathog. 2020 Nov 13;12(1):53. doi: 10.1186/s13099-020-00386-1. PMID: 33292444; PMCID: PMC7666523.

» View on PubMed

Schmidt JK, Keding LT, Block LN, et al. Placenta-derived macaque trophoblast stem cells: differentiation to syncytiotrophoblasts and extravillous trophoblasts reveals phenotypic reprogramming. Sci Rep. 2020 Nov 5;10(1):19159. doi: 10.1038/s41598-020-76313-w. PMID: 33154556; PMCID: PMC7644694.

» View on Scientific Reports

Kong H, Reczek CR, McElroy GS, et al. Metabolic determinants of cellular fitness dependent on mitochondrial reactive oxygen species. Sci Adv. 2020 Nov 4;6(45):eabb7272. doi: 10.1126/sciadv.abb7272. PMID: 33148642.

» View on PubMed

View More

Ghost Dye™ Red 780 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Red 780 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

 

Recent Publications: 

Stephens WZ, Kubinak JL, Ghazaryan A, Bauer KM, Bell R, Buhrke K, Chiaro TR, Weis AM, Tang WW, Monts JK, Soto R, Ekiz HA, O'Connell RM, Round JL. Epithelial-myeloid exchange of MHC class II constrains immunity and microbiota composition. Cell Rep. 2021 Nov 2;37(5):109916. doi: 10.1016/j.celrep.2021.109916. PMID: 34731608.

» View on ScienceDirect

Fiege JK, Block KE, Pierson MJ, Nanda H, Shepherd FK, Mickelson CK, Stolley JM, Matchett WE, Wijeyesinghe S, Meyerholz DK, Vezys V, Shen SS, Hamilton SE, Masopust D, Langlois RA. Mice with diverse microbial exposure histories as a model for preclinical vaccine testing. Cell Host Microbe. 2021 Oct 27:S1931-3128(21)00463-7. doi: 10.1016/j.chom.2021.10.001. Epub ahead of print. PMID: 34731647.

 » View on ScienceDirect

Sturmlechner I, Zhang C, Sine CC, van Deursen EJ, Jeganathan KB, Hamada N, Grasic J, Friedman D, Stutchman JT, Can I, Hamada M, Lim DY, Lee JH, Ordog T, Laberge RM, Shapiro V, Baker DJ, Li H, van Deursen JM. p21 produces a bioactive secretome that places stressed cells under immunosurveillance. Science. 2021 Oct 29;374(6567):eabb3420. doi: 10.1126/science.abb3420. Epub 2021 Oct 29. PMID: 34709885.

 » View on Science

Ayupe AC, Beckedorff F, Levay K, Yon B, Salgueiro Y, Shiekhattar R, Park KK. Identification of long noncoding RNAs in injury-resilient and injury-susceptible mouse retinal ganglion cells. BMC Genomics. 2021 Oct 14;22(1):741. doi: 10.1186/s12864-021-08050-x. PMID: 34649511.

» View on PubMed

Tei R, Iijima K, Matsumoto K, et al. TLR3-driven IFN-β antagonizes STAT5-activating cytokines and suppresses innate type 2 response in the lung. J Allergy Clin Immunol. 2021 Aug 21:S0091-6749(21)01296-3. doi: 10.1016/j.jaci.2021.07.041. PMID: 34428519.

» View on ScienceDirect

Magee K, Marsh IR, Turek MM, et al. Safety and feasibility of an in situ vaccination and immunomodulatory targeted radionuclide combination immuno-radiotherapy approach in a comparative (companion dog) setting. PLoS One. 2021 Aug 12;16(8):e0255798. doi: 10.1371/journal.pone.0255798. PMID: 34383787; PMCID: PMC8360580.

» View on PubMed

Jairaman A, Othy S, Dynes JL, et al. Piezo1 channels restrain regulatory T cells but are dispensable for effector CD4+ T cell responses. Sci Adv. 2021 Jul 7;7(28):eabg5859. doi: 10.1126/sciadv.abg5859. PMID: 34233878.

» View on PubMed

Burbach BJ, O'Flanagan SD, Shao Q, et al. Irreversible electroporation augments checkpoint immunotherapy in prostate cancer and promotes tumor antigen-specific tissue-resident memory CD8+ T cells. Nat Commun. 2021 Jun 23;12(1):3862. doi: 10.1038/s41467-021-24132-6. PMID: 34162858.

» View on NatureCommunications

Hamilton JR, Tsuchida CA, Nguyen DN, et al. Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering. Cell Rep. 2021 Jun 1;35(9):109207. doi: 10.1016/j.celrep.2021.109207. PMID: 34077734.

 » View on ScienceDirect

Davenport BJ, Morrison TE, Kedl RM, Klarquist J. Conserved and Novel Mouse CD8 T Cell Epitopes within SARS-CoV-2 Spike Receptor Binding Domain Protein Identified following Subunit Vaccination. J Immunol. 2021 May 10:ji2100195. doi: 10.4049/jimmunol.2100195. PMID: 33972373.

» View on JournalofImmunology

Trott DW, Islam MT, Buckley DJ, Donato AJ, Dutson T, Sorensen ES, Cai J, Gogulamudi VR, Phuong TTT, Lesniewski LA. T lymphocyte depletion ameliorates age-related metabolic impairments in mice. Geroscience. 2021 Apr 24. doi: 10.1007/s11357-021-00368-4. PMID: 33893902.

» View on PubMed

Marín-Jiménez JA, Capasso A, Lewis MS, et al. Testing Cancer Immunotherapy in a Human Immune System Mouse Model: Correlating Treatment Responses to Human Chimerism, Therapeutic Variables and Immune Cell Phenotypes. Front Immunol. 2021 Mar 29;12:607282. doi: 10.3389/fimmu.2021.607282. PMID: 33854497; PMCID: PMC8040953.

» View on PubMed

Lee W, Kingstad-Bakke B, Kedl RM, Kawaoka Y, Suresh M. CCR2 Regulates Vaccine-Induced Mucosal T-Cell Memory to Influenza A Virus. bioRxiv. 2021 Mar 25:2021.03.24.436901. doi: 10.1101/2021.03.24.436901. PMID: 33791695; PMCID: PMC8010722.

» View on PubMed

Mitchell JE, Lund MM, Starmer J, Ge K, Magnuson T, Shpargel KB, Whitmire JK. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021 Apr 13;35(2):108966. doi: 10.1016/j.celrep.2021.108966. PMID: 33852868.

» View on ScienceDirect

Reimann M, Schrezenmeier JF, Richter-Pechanska P, Dolnik A, et al. Adaptive T-cell immunity controls senescence-prone MyD88- or CARD11-mutant B-cell lymphomas. Blood. 2020 Nov 24:blood.2020005244. doi: 10.1182/blood.2020005244. PMID: 33232972.

» View on PubMed

McCoy SS, Giri J, Das R, et al. Minor salivary gland mesenchymal stromal cells derived from patients with Sjӧgren's syndrome deploy intact immune plasticity. Cytotherapy. 2020 Nov 28:S1465-3249(20)30861-6. doi: 10.1016/j.jcyt.2020.09.008. PMID: 33262072.

» View on PubMed

Fisher C, Patel R. Rifampin, Rifapentine, and Rifabutin are Active Against Intracellular Periprosthetic Joint Infection-Associated Staphylococcus epidermidis. Antimicrob Agents Chemother. 2020 Nov 16:AAC.01275-20. doi: 10.1128/AAC.01275-20. PMID: 33199387.

» View on PubMed

Bescucci DM, Clarke ST, Brown CLJ, et al. The absence of murine cathelicidin-related antimicrobial peptide impacts host responses enhancing Salmonella enterica serovar Typhimurium infection. Gut Pathog. 2020 Nov 13;12(1):53. doi: 10.1186/s13099-020-00386-1. PMID: 33292444; PMCID: PMC7666523.

» View on PubMed

Schmidt JK, Keding LT, Block LN, et al. Placenta-derived macaque trophoblast stem cells: differentiation to syncytiotrophoblasts and extravillous trophoblasts reveals phenotypic reprogramming. Sci Rep. 2020 Nov 5;10(1):19159. doi: 10.1038/s41598-020-76313-w. PMID: 33154556; PMCID: PMC7644694.

» View on Scientific Reports

Kong H, Reczek CR, McElroy GS, et al. Metabolic determinants of cellular fitness dependent on mitochondrial reactive oxygen species. Sci Adv. 2020 Nov 4;6(45):eabb7272. doi: 10.1126/sciadv.abb7272. PMID: 33148642.

» View on PubMed

Hide

Product Details

Name Ghost Dye™ Violet 780
Cat. No. 13-0865
Protocol Download Protocol
Excitation Laser red (633-640 nm)
Emission (nm) 780
Formulation 1 µL/test in DMSO
Application Flow Cytometry
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.