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LEFT: Mouse thymocytes were incubated overnight at 4°C (dashed) or 37°C (solid) and stained with Ghost Red 780.   RIGHT:  Mouse splenocytes were stimulated overnight with PMA and stained with Ghost Red 780. Viable gate is indicated.
  • LEFT: Mouse thymocytes were incubated overnight at 4°C (dashed) or 37°C (solid) and stained with Ghost Red 780.   RIGHT:  Mouse splenocytes were stimulated overnight with PMA and stained with Ghost Red 780. Viable gate is indicated.
  • Ghost Dye™ Red 780

Ghost Dye™ Red 780

Cat No. Size Price Quantity
13-0865-T100 100 tests $44.00
13-0865-T500 500 tests (5 x 100 tests) $170.00

Description

Ghost Dye™ Red 780 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Red 780 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

Recent Publications:

Braun MW, Abdelhakim H, Li M, Hyter S, Pessetto Z, Koestler DC, Pathak HB, Dunavin N, Godwin AK. Adherent cell depletion promotes the expansion of renal cell carcinoma infiltrating T cells with optimal characteristics for adoptive transfer. J Immunother Cancer. 2020 Oct;8(2):e000706. doi: 10.1136/jitc-2020-000706. PMID: 33037114.

» View on PubMed

Wise RM, Harrison MAA, Sullivan BN, Al-Ghadban S, Aleman SJ, Vinluan AT, Monaco ER, Donato UM, Pursell IA, Bunnell BA. Short-Term Rapamycin Preconditioning Diminishes Therapeutic Efficacy of Human Adipose-Derived Stem Cells in a Murine Model of Multiple Sclerosis. Cells. 2020 Sep 30;9(10):E2218. doi: 10.3390/cells9102218. PMID: 33008073.

» View on PubMed

Sakamoto A, Maruyama M. Contribution of DOCK11 to the Expansion of Antigen-Specific Populations among Germinal Center B Cells. Immunohorizons. 2020;4(9):520-529. Published 2020 Sep 2. doi:10.4049/immunohorizons.2000048

» View on PubMed

Rodríguez-Rodríguez N, Madera-Salcedo IK, Cisneros-Segura JA, et al. Protein phosphatase 2A B55ß limits CD8 T cell lifespan following cytokine withdrawal. 2020 Aug 4. J Clin Invest. 2020;129479. doi:10.1172/JCI129479. 

» View on PubMed

Symonds AL, Zheng W, Miao T, et al. Egr2 and 3 control inflammation, but maintain homeostasis, of PD-1high memory phenotype CD4 T cells. Life Sci Alliance. 2020;3(9):e202000766. Published 2020 Jul 24. doi:10.26508/lsa.202000766.

» View on PubMed

Nagayama M, Yano T, Atarashi K, et al. TH1 cell-inducing Escherichia coli strain identified from the small intestinal mucosa of patients with Crohn's disease [published online ahead of print, 2020 Jul 20]. Gut Microbes. 2020;1-14. doi:10.1080/19490976.2020.1788898.

» View on PubMed

Clark PA, Sriramaneni RN, Jin WJ, et al.  In situ vaccination at a peripheral tumor site augments response against melanoma brain metastases. Journal for ImmunoTherapy of Cancer 2020;8:e000809. doi: 10.1136/jitc-2020-000809.

» View on BMJ Journals

Andrea PennatiEmily A Nylen, Ian D Duncan, Jacques Galipeau . Regulatory B Cells Normalize CNS Myeloid Cell Content in a Mouse Model of Multiple Sclerosis and Promote Oligodendrogenesis and Remyelination. J Neuroscience. 2020 May 19;JN-RM-2840-19. doi: 10.1523/JNEUROSCI.2840-19.2020. 

» View on PubMed

Wormsbaecher C, Hindman AR, Avendano A, et al. In utero estrogenic endocrine disruption alters the stroma to increase extracellular matrix density and mammary gland stiffness. Breast Cancer Res. 2020;22(1):41. Published 2020 May 5. doi:10.1186/s13058-020-01275-w.

» View on PubMed

Giri J, Galipeau J. Mesenchymal stromal cell therapeutic potency is dependent upon viability, route of delivery, and immune match. Blood Adv. 2020 May 12;4(9):1987-1997. doi: 10.1182/bloodadvances.2020001711.

» View on PubMed

Roth TL, Li PJ, Blaeschke F, Nies JF, Apathy R, Mowery C, Yu R, Nguyen MLT, Lee Y, Truong A, Hiatt J, Wu D, Nguyen DN, Goodman D, Bluestone JA, Ye CJ, Roybal K, Shifrut E, Marson A. Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies. Cell. 2020 Apr 15. pii: S0092-8674(20)30332-9. doi: 10.1016/j.cell.2020.03.039.

» View on ScienceDirect

Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059

» View on PubMed

Jessica K. Fiege, Ian A. Stone, Elizabeth J. Fay, Matthew W. Markman, Sathi Wijeyesinghe, Marissa G. Macchietto, Steven Shen, David Masopust and Ryan A. Langlois. The Impact of TCR Signal Strength on Resident Memory T Cell Formation during Influenza Virus Infection. J Immunol. 2019 Aug 15;203(4):936-945. doi: 10.4049/jimmunol.1900093

» View on PubMed

Jessica K. Fiege, Ian A. Stone, Rebekah E. Dumm, Barbara M. Waring, Brian T. FifeI, Judith Agudo, Brian D. Brown, Nicholas S. Heaton, Ryan A. Langlois. Long-term surviving influenza infected cells evade CD8+ T cell mediated clearance. PLoS Pathog. 2019 Sep 26;15(9):e1008077. doi: 10.1371/journal.ppat.1008077

» View on PubMed

Matthew A. Huggins, Frances V. Sjaastad, Mark Pierson, Tamara A. Kucaba, Whitney Swanson, Christopher Staley, Alexa R. Weingarden, Isaac J. Jensen, Derek B. Danahy, Vladimir P. Badovinac, Stephen C. Jameson, Vaiva Vezys, David Masopust, Alexander Khoruts, Thomas S. Griffith, and Sara E. Hamilton. Microbial Exposure Enhances Immunity to Pathogens Recognized by TLR2 but Increases Susceptibility to Cytokine Storm through TLR4 Sensitization. Cell Rep. 2019 Aug 13;28(7):1729-1743.e5. doi: 10.1016/j.celrep.2019.07.028

» View on PubMed

Uzodinma U. Uche, Ann R. Piccirillo, Shunsuke Kataoka, Stephanie J. Grebinoski, Louise M. D’Cruz, and Lawrence P. Kane. PIK3IP1/TrIP restricts activation of T cells through inhibition of PI3K/Akt. J Exp Med. 2018 Dec 3;215(12):3165-3179. doi: 10.1084/jem.20172018

» View on PubMed

Guo M, Price MJ, Patterson DG, et al. EZH2 Represses the B Cell Transcriptional Program and Regulates Antibody-Secreting Cell Metabolism and Antibody Production. J Immunol. 2018;200(3):1039-1052. doi:10.4049/jimmunol.1701470.

» View on PubMed

Manan M. Mehta, Samuel E. Weinberg, Elizabeth M. Steinert, Krishan Chhiba, Carlos Alberto Martinez, Peng Gao, Harris R. Perlman, Paul Bryce, Nissim Hay, and Navdeep S. Chandel. Hexokinase 2 is dispensable for T cell-dependent immunity. Cancer Metab. 2018 Aug 17;6:10. doi: 10.1186/s40170-018-0184-5

» View on PubMed

Pushpak Bhattacharjee & Peter A. Keyel. Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses. Sci Rep. 2018 Apr 4;8(1):6458. doi: 10.1038/s41598-018-24955-2

» View on PubMed

Scharer CD, Barwick BG, Guo M, Bally APR, Boss JM. Plasma cell differentiation is controlled by multiple cell division-coupled epigenetic programs. Nat Commun. 2018;9(1):1698. Published 2018 Apr 27. doi:10.1038/s41467-018-04125-8.

» View on PubMed

Guthmiller JJ, Zander RA and Butler NS. 2015. Methods Mol Biol. 1325: 19-37. (Flow Cytometry)

Malhotra D, Linehan JL, Dileepan T, Lee YJ, Purtha WE, Lu JV, Nelson RW, Fife BT, Orr HT, Anderson MS, Hogquist KA and Jenkins MK. 2016. Nat Immunol. doi: 10.1038/ni.3327. (Flow Cytometry)

View More
Ghost Dye™ Red 780 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Red 780 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

Recent Publications:

Braun MW, Abdelhakim H, Li M, Hyter S, Pessetto Z, Koestler DC, Pathak HB, Dunavin N, Godwin AK. Adherent cell depletion promotes the expansion of renal cell carcinoma infiltrating T cells with optimal characteristics for adoptive transfer. J Immunother Cancer. 2020 Oct;8(2):e000706. doi: 10.1136/jitc-2020-000706. PMID: 33037114.

» View on PubMed

Wise RM, Harrison MAA, Sullivan BN, Al-Ghadban S, Aleman SJ, Vinluan AT, Monaco ER, Donato UM, Pursell IA, Bunnell BA. Short-Term Rapamycin Preconditioning Diminishes Therapeutic Efficacy of Human Adipose-Derived Stem Cells in a Murine Model of Multiple Sclerosis. Cells. 2020 Sep 30;9(10):E2218. doi: 10.3390/cells9102218. PMID: 33008073.

» View on PubMed

Sakamoto A, Maruyama M. Contribution of DOCK11 to the Expansion of Antigen-Specific Populations among Germinal Center B Cells. Immunohorizons. 2020;4(9):520-529. Published 2020 Sep 2. doi:10.4049/immunohorizons.2000048

» View on PubMed

Rodríguez-Rodríguez N, Madera-Salcedo IK, Cisneros-Segura JA, et al. Protein phosphatase 2A B55ß limits CD8 T cell lifespan following cytokine withdrawal. 2020 Aug 4. J Clin Invest. 2020;129479. doi:10.1172/JCI129479. 

» View on PubMed

Symonds AL, Zheng W, Miao T, et al. Egr2 and 3 control inflammation, but maintain homeostasis, of PD-1high memory phenotype CD4 T cells. Life Sci Alliance. 2020;3(9):e202000766. Published 2020 Jul 24. doi:10.26508/lsa.202000766.

» View on PubMed

Nagayama M, Yano T, Atarashi K, et al. TH1 cell-inducing Escherichia coli strain identified from the small intestinal mucosa of patients with Crohn's disease [published online ahead of print, 2020 Jul 20]. Gut Microbes. 2020;1-14. doi:10.1080/19490976.2020.1788898.

» View on PubMed

Clark PA, Sriramaneni RN, Jin WJ, et al.  In situ vaccination at a peripheral tumor site augments response against melanoma brain metastases. Journal for ImmunoTherapy of Cancer 2020;8:e000809. doi: 10.1136/jitc-2020-000809.

» View on BMJ Journals

Andrea PennatiEmily A Nylen, Ian D Duncan, Jacques Galipeau . Regulatory B Cells Normalize CNS Myeloid Cell Content in a Mouse Model of Multiple Sclerosis and Promote Oligodendrogenesis and Remyelination. J Neuroscience. 2020 May 19;JN-RM-2840-19. doi: 10.1523/JNEUROSCI.2840-19.2020. 

» View on PubMed

Wormsbaecher C, Hindman AR, Avendano A, et al. In utero estrogenic endocrine disruption alters the stroma to increase extracellular matrix density and mammary gland stiffness. Breast Cancer Res. 2020;22(1):41. Published 2020 May 5. doi:10.1186/s13058-020-01275-w.

» View on PubMed

Giri J, Galipeau J. Mesenchymal stromal cell therapeutic potency is dependent upon viability, route of delivery, and immune match. Blood Adv. 2020 May 12;4(9):1987-1997. doi: 10.1182/bloodadvances.2020001711.

» View on PubMed

Roth TL, Li PJ, Blaeschke F, Nies JF, Apathy R, Mowery C, Yu R, Nguyen MLT, Lee Y, Truong A, Hiatt J, Wu D, Nguyen DN, Goodman D, Bluestone JA, Ye CJ, Roybal K, Shifrut E, Marson A. Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies. Cell. 2020 Apr 15. pii: S0092-8674(20)30332-9. doi: 10.1016/j.cell.2020.03.039.

» View on ScienceDirect

Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059

» View on PubMed

Jessica K. Fiege, Ian A. Stone, Elizabeth J. Fay, Matthew W. Markman, Sathi Wijeyesinghe, Marissa G. Macchietto, Steven Shen, David Masopust and Ryan A. Langlois. The Impact of TCR Signal Strength on Resident Memory T Cell Formation during Influenza Virus Infection. J Immunol. 2019 Aug 15;203(4):936-945. doi: 10.4049/jimmunol.1900093

» View on PubMed

Jessica K. Fiege, Ian A. Stone, Rebekah E. Dumm, Barbara M. Waring, Brian T. FifeI, Judith Agudo, Brian D. Brown, Nicholas S. Heaton, Ryan A. Langlois. Long-term surviving influenza infected cells evade CD8+ T cell mediated clearance. PLoS Pathog. 2019 Sep 26;15(9):e1008077. doi: 10.1371/journal.ppat.1008077

» View on PubMed

Matthew A. Huggins, Frances V. Sjaastad, Mark Pierson, Tamara A. Kucaba, Whitney Swanson, Christopher Staley, Alexa R. Weingarden, Isaac J. Jensen, Derek B. Danahy, Vladimir P. Badovinac, Stephen C. Jameson, Vaiva Vezys, David Masopust, Alexander Khoruts, Thomas S. Griffith, and Sara E. Hamilton. Microbial Exposure Enhances Immunity to Pathogens Recognized by TLR2 but Increases Susceptibility to Cytokine Storm through TLR4 Sensitization. Cell Rep. 2019 Aug 13;28(7):1729-1743.e5. doi: 10.1016/j.celrep.2019.07.028

» View on PubMed

Uzodinma U. Uche, Ann R. Piccirillo, Shunsuke Kataoka, Stephanie J. Grebinoski, Louise M. D’Cruz, and Lawrence P. Kane. PIK3IP1/TrIP restricts activation of T cells through inhibition of PI3K/Akt. J Exp Med. 2018 Dec 3;215(12):3165-3179. doi: 10.1084/jem.20172018

» View on PubMed

Guo M, Price MJ, Patterson DG, et al. EZH2 Represses the B Cell Transcriptional Program and Regulates Antibody-Secreting Cell Metabolism and Antibody Production. J Immunol. 2018;200(3):1039-1052. doi:10.4049/jimmunol.1701470.

» View on PubMed

Manan M. Mehta, Samuel E. Weinberg, Elizabeth M. Steinert, Krishan Chhiba, Carlos Alberto Martinez, Peng Gao, Harris R. Perlman, Paul Bryce, Nissim Hay, and Navdeep S. Chandel. Hexokinase 2 is dispensable for T cell-dependent immunity. Cancer Metab. 2018 Aug 17;6:10. doi: 10.1186/s40170-018-0184-5

» View on PubMed

Pushpak Bhattacharjee & Peter A. Keyel. Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses. Sci Rep. 2018 Apr 4;8(1):6458. doi: 10.1038/s41598-018-24955-2

» View on PubMed

Scharer CD, Barwick BG, Guo M, Bally APR, Boss JM. Plasma cell differentiation is controlled by multiple cell division-coupled epigenetic programs. Nat Commun. 2018;9(1):1698. Published 2018 Apr 27. doi:10.1038/s41467-018-04125-8.

» View on PubMed

Guthmiller JJ, Zander RA and Butler NS. 2015. Methods Mol Biol. 1325: 19-37. (Flow Cytometry)

Malhotra D, Linehan JL, Dileepan T, Lee YJ, Purtha WE, Lu JV, Nelson RW, Fife BT, Orr HT, Anderson MS, Hogquist KA and Jenkins MK. 2016. Nat Immunol. doi: 10.1038/ni.3327. (Flow Cytometry)

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Product Details

Name Ghost Dye™ Red 780
Cat. No. 13-0865
Protocol Download Protocol
Excitation Laser Red (633 nm)
Emission (nm) 780
Formulation 1 µL/test in DMSO
Application Flow Cytometry
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.