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Mouse splenocytes were stimulated overnight with PMA and ionomycin and stained with Ghost Dye™ Red 710. Viable gate is indicated.
  • Mouse splenocytes were stimulated overnight with PMA and ionomycin and stained with Ghost Dye™ Red 710. Viable gate is indicated.
  • Ghost Dye™ Red 710

Ghost Dye™ Red 710

Cat No. Size Price Quantity
13-0871-T100 100 tests $44.00
13-0871-T500 500 tests (5 x 100 tests) $170.00

Description

Ghost Dye™ Red 710 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Red 710 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.


Recent Publications:

Chronic unilateral cervical vagotomy reduces renal inflammation, blood pressure, and renal injury in a mouse model of lupus. Pham GS, Gusson Shimoura C, Chaudhari S, Kulp DV, Mathis KW. 2020 Jun 15. Am J Physiol Renal Physiol. 2020;10.1152/ajprenal.00201.2020. doi:10.1152/ajprenal.00201.2020.

 » View on PubMed

Zhichao Fan, Zbigniew Mikulski, Sara McArdle, Prithu Sundd, Klaus Ley. Super-STORM: Molecular Modeling to Achieve Single-molecule Localization with STORM Microscopy. ScienceDirect. 2020 June 3: https://doi.org/10.1016/j.xpro.2019.100012.

» View on ScienceDirect

Rita Kansal, Noah Richardson, Indira Neeli, Saleem Khawaja, Damian Chamberlain, Marium Ghani, Qurat-ul-ain Ghani, Louisa Balazs, Sarka Beranova-Giorgianni, Francesco Giorgianni, James N. Kochenderfer, Tony Marion, Lorraine M. Albritton, Marko Radic. Sustained B cell depletion by CD19-targeted CAR T cells is a highly effective treatment for murine lupus. Sci Transl Med. 2019 Mar 6;11(482). pii: eaav1648. doi: 10.1126/scitranslmed.aav1648

» View on PubMed

Name Ghost Dye™ Red 710
Cat. No. 13-0871
Protocol Download Protocol
Excitation Laser Red (633-647 nm)
Emission (nm) 710
Formulation 1 µL/test in DMSO
Application Flow Cytometry
View More

Ghost Dye™ Red 710 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Red 710 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.


Recent Publications:

Chronic unilateral cervical vagotomy reduces renal inflammation, blood pressure, and renal injury in a mouse model of lupus. Pham GS, Gusson Shimoura C, Chaudhari S, Kulp DV, Mathis KW. 2020 Jun 15. Am J Physiol Renal Physiol. 2020;10.1152/ajprenal.00201.2020. doi:10.1152/ajprenal.00201.2020.

 » View on PubMed

Zhichao Fan, Zbigniew Mikulski, Sara McArdle, Prithu Sundd, Klaus Ley. Super-STORM: Molecular Modeling to Achieve Single-molecule Localization with STORM Microscopy. ScienceDirect. 2020 June 3: https://doi.org/10.1016/j.xpro.2019.100012.

» View on ScienceDirect

Rita Kansal, Noah Richardson, Indira Neeli, Saleem Khawaja, Damian Chamberlain, Marium Ghani, Qurat-ul-ain Ghani, Louisa Balazs, Sarka Beranova-Giorgianni, Francesco Giorgianni, James N. Kochenderfer, Tony Marion, Lorraine M. Albritton, Marko Radic. Sustained B cell depletion by CD19-targeted CAR T cells is a highly effective treatment for murine lupus. Sci Transl Med. 2019 Mar 6;11(482). pii: eaav1648. doi: 10.1126/scitranslmed.aav1648

» View on PubMed

Name Ghost Dye™ Red 710
Cat. No. 13-0871
Protocol Download Protocol
Excitation Laser Red (633-647 nm)
Emission (nm) 710
Formulation 1 µL/test in DMSO
Application Flow Cytometry
Hide

Product Details

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.