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Mouse bone marrow cells were heat killed at 65°C for 10 minutes and then mixed with live mouse bone marrow cells. Cells were then stained with Ghost Dye™ Blue 516. Viable gate is indicated.
  • Mouse bone marrow cells were heat killed at 65°C for 10 minutes and then mixed with live mouse bone marrow cells. Cells were then stained with Ghost Dye™ Blue 516. Viable gate is indicated.

Ghost Dye™ Blue 516

Cat No. Size Price Quantity
13-0867-T100 100 tests $44.00
13-0867-T500 500 tests (5 x 100 tests) $170.00

Description

Ghost Dye™ Blue 516 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Blue 516 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.
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Ghost Dye™ Blue 516 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ Blue 516 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.
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Product Details

Name Ghost Dye™ Blue 516
Cat. No. 13-0867
Protocol Download Protocol
Excitation Laser 488 nm
Emission (nm) 516
Formulation 1 µL/test in DMSO
Application Flow Cytometry
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.