Foxp3 / Transcription Factor Staining Buffer Kit
  • Foxp3 / Transcription Factor Staining Buffer Kit

Foxp3 / Transcription Factor Staining Buffer Kit

Cat No. Size Price Quantity
TNB-0607-KIT Kit $118.00

Description

Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry. Included are the following components, to be used during staining protocols for detection of nuclear antigens such as Foxp3 and ROR gamma.

Foxp3 / Transcription Factor Fix/Perm Concentrate (4X) (Cat. No. TNB-1020-L050): 50 mL. A concentrated solution which, when diluted with Foxp3 / Transcription Factor Fix/Perm Diluent (1X) (cat. no. TNB-1022-L160), provide best results during intracellular staining of transcription factors using fluorescently conjugated antibodies.

Foxp3 / Transcription Factor Fix/Perm Diluent (1X) (Cat. No. TNB-1022-L160): 160 mL. Intended for use as a diluent for Foxp3 / Transcription Factor Fix/Perm Concentrate (4X)

Flow Cytometry Perm Buffer (10X) (Cat. No. TNB-1213-L150): 150 mL. Provided as a concentrate which, when diluted with distilled water to a 1X solution, provide best results during intracellular staining of cytokines and other cytoplasmic antigens, by maintaining membrane permeabilization throughout staining and washing steps.

Recent Publications:
 

Lei Y, Guerra Martinez C, Torres-Odio S, et al. Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice. Sci Adv. 2021 May 26;7(22):eabe7548. doi: 10.1126/sciadv.abe7548. PMID: 34039599.

» View on PubMed

Kim DI, Cho YB, Lim Y, Hong SH, Hahm B, Lee SM, Kang SC, Seo YJ. Chios mastic gum inhibits influenza A virus replication and viral pathogenicity. J Gen Virol. 2021 Jan 8. doi: 10.1099/jgv.0.001550. PMID: 33416468.  

» View on PubMed

Kumar P, Arbieva ZH, Maienschein-Cline M, Ganesh BB, Ramasamy S, Prabhakar BS. Induction of Antigen-Independent Proliferation of Regulatory T-Cells by TNF Superfamily Ligands OX40L and GITRL. Methods Mol Biol. 2021;2248:63-71. doi: 10.1007/978-1-0716-1130-2_4. PMID: 33185867.

» View on PubMed

Poudel B, Yorek MS, Mazgaeen L, Brown SA, Kanneganti TD, Gurung P. Acute IL-4 Governs Pathogenic T Cell Responses during Leishmania major Infection. Immunohorizons. 2020 Sep 18;4(9):546-560. doi: 10.4049/immunohorizons.2000076. PMID: 32948646.

» View on PubMed

Mishra S, Liao W, Liu Y, Yang M, Ma C, Wu H, Zhao M, Zhang X, Qiu Y, Lu Q, Zhang N. TGF-β and Eomes control the homeostasis of CD8+ regulatory T cells. J Exp Med. 2021 Jan 4;218(1):e20200030. doi: 10.1084/jem.20200030. PMID: 32991667.  

» View on PubMed

Yashiro T, Yura S, Tobita A, Toyoda Y, Kasakura K, Nishiyama C. Pterostilbene reduces colonic inflammation by suppressing dendritic cell activation and promoting regulatory T cell development. FASEB J. 2020 Sep 22. doi: 10.1096/fj.202001502R. PMID: 32964554.  

» View on PubMed

Semionatto IF, Palameta S, Toscaro JM, Manrique-Rincón AJ, Ruas LP, Paes Leme AF, Bajgelman MC. Extracellular vesicles produced by immunomodulatory cells harboring OX40 ligand and 4-1BB ligand enhance antitumor immunity. Sci Rep. 2020 Sep 16;10(1):15160. doi: 10.1038/s41598-020-72122-3. PMID: 32939048.  

» View on Scientific Reports

Yagi Y, Kuwahara M, Suzuki J, Imai Y, Yamashita M. Glycolysis and subsequent mevalonate biosynthesis play an important role in Th2 cell differentiation. 2020 Aug 12. Biochem Biophys Res Commun. 2020;S0006-291X(20)31554-0. doi:10.1016/j.bbrc.2020.08.009.

 » View on ScienceDirect

Conor N. Gruber, Jorg J.A. Calis, Sofija Buta, et al. Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function. Immunity, 2020 Aug 3. https://doi.org/10.1016/j.immuni.2020.07.006

 » View on ScienceDirect

Long Y, Zhao X, Liu C, Xia C, Liu C. Activated inducible co-stimulator-positive (ICOS+ ) programmed death 1-positive (PD-1+ ) Tfh cells indicate disease activity and severity in ulcerative colitis patients [published online ahead of print, 2020 Jul 4]. Clin Exp Immunol. 2020;10.1111/cei.13485. doi:10.1111/cei.13485.

 » View on PubMed

J. Michael Stolley, Timothy S. Johnston, Andrew G. Soerens, Lalit K. Beura, Pamela C. Rosato, Vineet Joag, Sathi P. Wijeyesinghe, Ryan A. Langlois, Kevin C. Osum, Jason S. Mitchell, David Masopust; Retrograde migration supplies resident memory T cells to lung-draining LN after influenza infection. J Exp Med3 June 2020; 217 (8): e20192197. doi: https://doi.org/10.1084/jem.20192197.

 » View on PubMed

IL-6 Deficiency Exacerbates Allergic Asthma and Abrogates the Protective Effect of Allergic Inflammation against Streptococcus pneumoniae Pathogenesis. Taylor Schmit, Sumit Ghosh, Ram Kumar Mathur, Tyler Barnhardt, Ganesh Ambigapathy, Min Wu, Colin Combs, M. Nadeem Khan. The Journal of Immunology June 15, 2020, ji1900755; DOI: 10.4049/jimmunol.1900755.

  » View on Journal of Immunology

Correlation of the levels of DNA-binding inhibitor Id3 and regulatory T cells with SLE disease severity. ChenLiu. SenYu. RongJin. YanLong. SongsongLu. YingSong. XiuyuanSun. Xiao-HongSun. YuZhang. J Autoimmun2020 Jun 11;102498.  doi: 10.1016/j.jaut.2020.102498. 

 » View on ScienceDirect

Franco da Cunha F, Andrade-Oliveira V, Candido de Almeida D, Borges da Silva T, Naffah de Souza Breda C, Costa Cruz M, Faquim-Mauro EL, Antonio Cenedeze M, Ioshie Hiyane M, Pacheco-Silva A, Aparecida Cavinato R, Torrecilhas AC, Olsen Saraiva Câmara N. Extracellular Vesicles isolated from Mesenchymal Stromal Cells Modulate CD4+ T Lymphocytes Toward a Regulatory Profile. Cells. 2020 Apr 23;9(4). pii: E1059. doi: 10.3390/cells9041059Extracellular Vesicles isolated from Mesenchymal Stromal Cells Modulate CD4+ T Lymphocytes Toward a Regulatory Profile. Cells. 2020 Apr 23;9(4). pii: E1059. doi: 10.3390/cells9041059

» View on PubMed

Woolsey C, Jankeel A, Matassov D, et al. Immune correlates of postexposure vaccine protection against Marburg virus. Sci Rep. 2020;10(1):3071. Published 2020 Feb 20. doi:10.1038/s41598-020-59976-3.

 » View on Nature

Laura S. Angelo, Lynn H. Bimler, Rana Nikzad, Kevin Aviles-Padilla, and Silke Paust. CXCR6+ NK Cells in Human Fetal Liver and Spleen Possess Unique Phenotypic and Functional Capabilities. Front Immunol. 2019 Mar 19;10:469. doi: 10.3389/fimmu.2019.00469

» View on PubMed

Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059

» View on PubMed

Adami AJ, Bracken SJ, Guernsey LA, et al. Early-life antibiotics attenuate regulatory T cell generation and increase the severity of murine house dust mite-induced asthma. Pediatr Res. 2018;84(3):426-434. doi:10.1038/s41390-018-0031-y.

» View on PubMed

Karo JM, Schatz DG and Sun JC. 2014. Cell 159(1): 94-107. (Flow Cytometry)

Lambracht-Washington D and Rosenberg RN. 2015. Neurobiol Aging. 36(3): 1274-81. (Flow Cytometry)

Luo CT, Liao W, Dadi S, Toure A and Li MO. 2016. Nature. 529(7587): 532-536. (Flow Cytometry)

View More

Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry. Included are the following components, to be used during staining protocols for detection of nuclear antigens such as Foxp3 and ROR gamma.

Foxp3 / Transcription Factor Fix/Perm Concentrate (4X) (Cat. No. TNB-1020-L050): 50 mL. A concentrated solution which, when diluted with Foxp3 / Transcription Factor Fix/Perm Diluent (1X) (cat. no. TNB-1022-L160), provide best results during intracellular staining of transcription factors using fluorescently conjugated antibodies.

Foxp3 / Transcription Factor Fix/Perm Diluent (1X) (Cat. No. TNB-1022-L160): 160 mL. Intended for use as a diluent for Foxp3 / Transcription Factor Fix/Perm Concentrate (4X)

Flow Cytometry Perm Buffer (10X) (Cat. No. TNB-1213-L150): 150 mL. Provided as a concentrate which, when diluted with distilled water to a 1X solution, provide best results during intracellular staining of cytokines and other cytoplasmic antigens, by maintaining membrane permeabilization throughout staining and washing steps.

Recent Publications:
 

Lei Y, Guerra Martinez C, Torres-Odio S, et al. Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice. Sci Adv. 2021 May 26;7(22):eabe7548. doi: 10.1126/sciadv.abe7548. PMID: 34039599.

» View on PubMed

Kim DI, Cho YB, Lim Y, Hong SH, Hahm B, Lee SM, Kang SC, Seo YJ. Chios mastic gum inhibits influenza A virus replication and viral pathogenicity. J Gen Virol. 2021 Jan 8. doi: 10.1099/jgv.0.001550. PMID: 33416468.  

» View on PubMed

Kumar P, Arbieva ZH, Maienschein-Cline M, Ganesh BB, Ramasamy S, Prabhakar BS. Induction of Antigen-Independent Proliferation of Regulatory T-Cells by TNF Superfamily Ligands OX40L and GITRL. Methods Mol Biol. 2021;2248:63-71. doi: 10.1007/978-1-0716-1130-2_4. PMID: 33185867.

» View on PubMed

Poudel B, Yorek MS, Mazgaeen L, Brown SA, Kanneganti TD, Gurung P. Acute IL-4 Governs Pathogenic T Cell Responses during Leishmania major Infection. Immunohorizons. 2020 Sep 18;4(9):546-560. doi: 10.4049/immunohorizons.2000076. PMID: 32948646.

» View on PubMed

Mishra S, Liao W, Liu Y, Yang M, Ma C, Wu H, Zhao M, Zhang X, Qiu Y, Lu Q, Zhang N. TGF-β and Eomes control the homeostasis of CD8+ regulatory T cells. J Exp Med. 2021 Jan 4;218(1):e20200030. doi: 10.1084/jem.20200030. PMID: 32991667.  

» View on PubMed

Yashiro T, Yura S, Tobita A, Toyoda Y, Kasakura K, Nishiyama C. Pterostilbene reduces colonic inflammation by suppressing dendritic cell activation and promoting regulatory T cell development. FASEB J. 2020 Sep 22. doi: 10.1096/fj.202001502R. PMID: 32964554.  

» View on PubMed

Semionatto IF, Palameta S, Toscaro JM, Manrique-Rincón AJ, Ruas LP, Paes Leme AF, Bajgelman MC. Extracellular vesicles produced by immunomodulatory cells harboring OX40 ligand and 4-1BB ligand enhance antitumor immunity. Sci Rep. 2020 Sep 16;10(1):15160. doi: 10.1038/s41598-020-72122-3. PMID: 32939048.  

» View on Scientific Reports

Yagi Y, Kuwahara M, Suzuki J, Imai Y, Yamashita M. Glycolysis and subsequent mevalonate biosynthesis play an important role in Th2 cell differentiation. 2020 Aug 12. Biochem Biophys Res Commun. 2020;S0006-291X(20)31554-0. doi:10.1016/j.bbrc.2020.08.009.

 » View on ScienceDirect

Conor N. Gruber, Jorg J.A. Calis, Sofija Buta, et al. Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function. Immunity, 2020 Aug 3. https://doi.org/10.1016/j.immuni.2020.07.006

 » View on ScienceDirect

Long Y, Zhao X, Liu C, Xia C, Liu C. Activated inducible co-stimulator-positive (ICOS+ ) programmed death 1-positive (PD-1+ ) Tfh cells indicate disease activity and severity in ulcerative colitis patients [published online ahead of print, 2020 Jul 4]. Clin Exp Immunol. 2020;10.1111/cei.13485. doi:10.1111/cei.13485.

 » View on PubMed

J. Michael Stolley, Timothy S. Johnston, Andrew G. Soerens, Lalit K. Beura, Pamela C. Rosato, Vineet Joag, Sathi P. Wijeyesinghe, Ryan A. Langlois, Kevin C. Osum, Jason S. Mitchell, David Masopust; Retrograde migration supplies resident memory T cells to lung-draining LN after influenza infection. J Exp Med3 June 2020; 217 (8): e20192197. doi: https://doi.org/10.1084/jem.20192197.

 » View on PubMed

IL-6 Deficiency Exacerbates Allergic Asthma and Abrogates the Protective Effect of Allergic Inflammation against Streptococcus pneumoniae Pathogenesis. Taylor Schmit, Sumit Ghosh, Ram Kumar Mathur, Tyler Barnhardt, Ganesh Ambigapathy, Min Wu, Colin Combs, M. Nadeem Khan. The Journal of Immunology June 15, 2020, ji1900755; DOI: 10.4049/jimmunol.1900755.

  » View on Journal of Immunology

Correlation of the levels of DNA-binding inhibitor Id3 and regulatory T cells with SLE disease severity. ChenLiu. SenYu. RongJin. YanLong. SongsongLu. YingSong. XiuyuanSun. Xiao-HongSun. YuZhang. J Autoimmun2020 Jun 11;102498.  doi: 10.1016/j.jaut.2020.102498. 

 » View on ScienceDirect

Franco da Cunha F, Andrade-Oliveira V, Candido de Almeida D, Borges da Silva T, Naffah de Souza Breda C, Costa Cruz M, Faquim-Mauro EL, Antonio Cenedeze M, Ioshie Hiyane M, Pacheco-Silva A, Aparecida Cavinato R, Torrecilhas AC, Olsen Saraiva Câmara N. Extracellular Vesicles isolated from Mesenchymal Stromal Cells Modulate CD4+ T Lymphocytes Toward a Regulatory Profile. Cells. 2020 Apr 23;9(4). pii: E1059. doi: 10.3390/cells9041059Extracellular Vesicles isolated from Mesenchymal Stromal Cells Modulate CD4+ T Lymphocytes Toward a Regulatory Profile. Cells. 2020 Apr 23;9(4). pii: E1059. doi: 10.3390/cells9041059

» View on PubMed

Woolsey C, Jankeel A, Matassov D, et al. Immune correlates of postexposure vaccine protection against Marburg virus. Sci Rep. 2020;10(1):3071. Published 2020 Feb 20. doi:10.1038/s41598-020-59976-3.

 » View on Nature

Laura S. Angelo, Lynn H. Bimler, Rana Nikzad, Kevin Aviles-Padilla, and Silke Paust. CXCR6+ NK Cells in Human Fetal Liver and Spleen Possess Unique Phenotypic and Functional Capabilities. Front Immunol. 2019 Mar 19;10:469. doi: 10.3389/fimmu.2019.00469

» View on PubMed

Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059

» View on PubMed

Adami AJ, Bracken SJ, Guernsey LA, et al. Early-life antibiotics attenuate regulatory T cell generation and increase the severity of murine house dust mite-induced asthma. Pediatr Res. 2018;84(3):426-434. doi:10.1038/s41390-018-0031-y.

» View on PubMed

Karo JM, Schatz DG and Sun JC. 2014. Cell 159(1): 94-107. (Flow Cytometry)

Lambracht-Washington D and Rosenberg RN. 2015. Neurobiol Aging. 36(3): 1274-81. (Flow Cytometry)

Luo CT, Liao W, Dadi S, Toure A and Li MO. 2016. Nature. 529(7587): 532-536. (Flow Cytometry)

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Product Details

Name Foxp3 / Transcription Factor Staining Buffer Kit
Cat. No. TNB-0607
Protocol Foxp3 / Transcription Factor (Nuclear) Staining Protocol
Application Flow Cytometry
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.