Flow Staining Buffer (1X)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| TNB-4222-L500 | 500 mL | $60.00 |
Technical Data Sheet
Request Custom/BulkDescription
The Flow Staining Buffer (1X) is provided as a ready-to-use solution for use in immunofluorescent staining protocols using viable or fixed cells in suspension for flow cytometry. Flow Staining Buffer is a buffered saline solution containing Bovine Calf Serum as a protein source to minimize nonspecific binding of antibodies and sodium azide (0.09%) as a preservative. This buffer can be used for cell suspension, antibody dilution, and all wash steps needed for cell surface staining and flow cytometric analysis.
Recent Citations:
Burbach BJ, O'Flanagan SD, Shao Q, et al. Irreversible electroporation augments checkpoint immunotherapy in prostate cancer and promotes tumor antigen-specific tissue-resident memory CD8+ T cells. Nat Commun. 2021 Jun 23;12(1):3862. doi: 10.1038/s41467-021-24132-6. PMID: 34162858.
» View on NatureCommunications
Pingili AK, Chaib M, Sipe LM, et al. Immune checkpoint blockade reprograms systemic immune landscape and tumor microenvironment in obesity-associated breast cancer. Cell Rep. 2021 Jun 22;35(12):109285. doi: 10.1016/j.celrep.2021.109285. PMID: 34161764.
The Flow Staining Buffer (1X) is provided as a ready-to-use solution for use in immunofluorescent staining protocols using viable or fixed cells in suspension for flow cytometry. Flow Staining Buffer is a buffered saline solution containing Bovine Calf Serum as a protein source to minimize nonspecific binding of antibodies and sodium azide (0.09%) as a preservative. This buffer can be used for cell suspension, antibody dilution, and all wash steps needed for cell surface staining and flow cytometric analysis.
Recent Citations:
Burbach BJ, O'Flanagan SD, Shao Q, et al. Irreversible electroporation augments checkpoint immunotherapy in prostate cancer and promotes tumor antigen-specific tissue-resident memory CD8+ T cells. Nat Commun. 2021 Jun 23;12(1):3862. doi: 10.1038/s41467-021-24132-6. PMID: 34162858.
» View on NatureCommunications
Pingili AK, Chaib M, Sipe LM, et al. Immune checkpoint blockade reprograms systemic immune landscape and tumor microenvironment in obesity-associated breast cancer. Cell Rep. 2021 Jun 22;35(12):109285. doi: 10.1016/j.celrep.2021.109285. PMID: 34161764.
Product Details
| Name | Flow Staining Buffer (1X) |
|---|---|
| Cat. No. | TNB-4222 |
| Protocol | |
| Application | FC |
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.