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Flow Cytometry Perm Buffer (10X)
  • Flow Cytometry Perm Buffer (10X)

Flow Cytometry Perm Buffer (10X)

Cat No. Size Price Quantity
TNB-1213-L150 150 mL $63.00

Description

Tonbo Biosciences Flow Cytometry Perm Buffer (10X) is provided as a concentrate which, when diluted with distilled water to a 1X solution, provides best results during intracellular staining of cytokines and other cytoplasmic antigens, by maintaining membrane permeabilization throughout staining and washing steps. The Flow Cytometry Perm Buffer is formulated for optimal resolution and low background when used in fluorophore-labeled antibody staining protocols, followed by flow cytometric analysis.


Intracellular Cytokine Staining ProtocolDownload the Intracellular Cytokine Staining Protocol.

Recent Publications:

Jesser EA, Brady NJ, Huggins DN, Witschen PM, O'Connor CH, Schwertfeger KL. STAT5 is activated in macrophages by breast cancer cell-derived factors and regulates macrophage function in the tumor microenvironment. Breast Cancer Res. 2021 Nov 7;23(1):104. doi: 10.1186/s13058-021-01481-0. PMID: 34743736; PMCID: PMC8573892.

» View on PubMed

Xu K, Li MO. Delivery of membrane impermeable molecules to primary mouse T lymphocytes. STAR Protoc. 2021 Aug 17;2(3):100757. doi: 10.1016/j.xpro.2021.100757. PMID: 34458872; PMCID: PMC8377589.

» View on PubMed

Poudel B, Yorek MS, Mazgaeen L, Brown SA, Kanneganti TD, Gurung P. Acute IL-4 Governs Pathogenic T Cell Responses during Leishmania major Infection. Immunohorizons. 2020 Sep 18;4(9):546-560. doi: 10.4049/immunohorizons.2000076. PMID: 32948646.

» View on PubMed

Kotov JA, Kotov DI, Linehan JL, Bardwell VJ, Gearhart MD, Jenkins MK. BCL6 corepressor contributes to Th17 cell formation by inhibiting Th17 fate suppressors. J Exp Med. 2019;216(6):1450-1464. doi:10.1084/jem.20182376.

» View on PubMed

Kevin C. Osum, Adam L. Burrack, Tijana Martinov, Nathanael L. Sahli, Jason S. Mitchell, Christopher G. Tucker, Kristen E. Pauken, Klearchos Papas, Balamurugan Appakalai, Justin A. Spanier, & Brian T. Fife. Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes. Sci Rep. 2018 May 29;8(1):8295. doi: 10.1038/s41598-018-26471-9

» View on PubMed

Lesteberg K, Orange J and Makedonas G. 2017. Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin. Immunol Res. doi: 10.1007/s12026-017-8945-8.

» View on PubMed

Colombo E, Tentorio P, Musio S, Rajewsky K, Pedotti R, Casola S, and Farina C. 2014. Clin. Exp. Immunol. 176:58-65. (Flow Cytometry)

View More

Tonbo Biosciences Flow Cytometry Perm Buffer (10X) is provided as a concentrate which, when diluted with distilled water to a 1X solution, provides best results during intracellular staining of cytokines and other cytoplasmic antigens, by maintaining membrane permeabilization throughout staining and washing steps. The Flow Cytometry Perm Buffer is formulated for optimal resolution and low background when used in fluorophore-labeled antibody staining protocols, followed by flow cytometric analysis.


Intracellular Cytokine Staining ProtocolDownload the Intracellular Cytokine Staining Protocol.

Recent Publications:

Jesser EA, Brady NJ, Huggins DN, Witschen PM, O'Connor CH, Schwertfeger KL. STAT5 is activated in macrophages by breast cancer cell-derived factors and regulates macrophage function in the tumor microenvironment. Breast Cancer Res. 2021 Nov 7;23(1):104. doi: 10.1186/s13058-021-01481-0. PMID: 34743736; PMCID: PMC8573892.

» View on PubMed

Xu K, Li MO. Delivery of membrane impermeable molecules to primary mouse T lymphocytes. STAR Protoc. 2021 Aug 17;2(3):100757. doi: 10.1016/j.xpro.2021.100757. PMID: 34458872; PMCID: PMC8377589.

» View on PubMed

Poudel B, Yorek MS, Mazgaeen L, Brown SA, Kanneganti TD, Gurung P. Acute IL-4 Governs Pathogenic T Cell Responses during Leishmania major Infection. Immunohorizons. 2020 Sep 18;4(9):546-560. doi: 10.4049/immunohorizons.2000076. PMID: 32948646.

» View on PubMed

Kotov JA, Kotov DI, Linehan JL, Bardwell VJ, Gearhart MD, Jenkins MK. BCL6 corepressor contributes to Th17 cell formation by inhibiting Th17 fate suppressors. J Exp Med. 2019;216(6):1450-1464. doi:10.1084/jem.20182376.

» View on PubMed

Kevin C. Osum, Adam L. Burrack, Tijana Martinov, Nathanael L. Sahli, Jason S. Mitchell, Christopher G. Tucker, Kristen E. Pauken, Klearchos Papas, Balamurugan Appakalai, Justin A. Spanier, & Brian T. Fife. Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes. Sci Rep. 2018 May 29;8(1):8295. doi: 10.1038/s41598-018-26471-9

» View on PubMed

Lesteberg K, Orange J and Makedonas G. 2017. Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin. Immunol Res. doi: 10.1007/s12026-017-8945-8.

» View on PubMed

Colombo E, Tentorio P, Musio S, Rajewsky K, Pedotti R, Casola S, and Farina C. 2014. Clin. Exp. Immunol. 176:58-65. (Flow Cytometry)

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Product Details

Name Flow Cytometry Perm Buffer (10X)
Cat. No. TNB-1213
Protocol Intracellular Cytokine Staining Protocol
Application Flow Cytometry
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.