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C57Bl/6 spleen cells were stained with APC Anti-Mouse CD8a (20-0081) and 0.25 ug FITC Anti-Mouse Ly-6C (35-5932) (right panel) or 0.25 ug FITC Rat IgG2c isotype control (left panel).
  • C57Bl/6 spleen cells were stained with APC Anti-Mouse CD8a (20-0081) and 0.25 ug FITC Anti-Mouse Ly-6C (35-5932) (right panel) or 0.25 ug FITC Rat IgG2c isotype control (left panel).

FITC Anti-Mouse Ly-6C (HK1.4)

Cat No. Size Price Quantity
35-5932-U025 25 µg $35.00
35-5932-U100 100 µg $80.00

Description

The HK1.4 monoclonal antibody reacts with mouse Ly-6C, a 15-17kD GPI-linked cell-surface protein. Ly-6C is expressed on monocyte/macrophage cells, endothelial cells, granulocytes and a subset of lymphocytes. Mice strains with the Ly-6.2 alloantigen have subsets of both Ly-6C positive CD4 and CD8 cells while Ly-6.1 expressing strains have only the CD8 Ly-6C positive subset.

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The HK1.4 monoclonal antibody reacts with mouse Ly-6C, a 15-17kD GPI-linked cell-surface protein. Ly-6C is expressed on monocyte/macrophage cells, endothelial cells, granulocytes and a subset of lymphocytes. Mice strains with the Ly-6.2 alloantigen have subsets of both Ly-6C positive CD4 and CD8 cells while Ly-6.1 expressing strains have only the CD8 Ly-6C positive subset.

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Product Details

Name FITC Anti-Mouse Ly-6C (HK1.4)
Cat. No. 35-5932
Alternative Names Ly6C
Gene ID 16398
Clone HK1.4
Isotype Rat IgG2c, kappa
Reactivity Mouse
Cross Reactivity
Format FITC
Application Flow Cytometry
Citations* Havran WL, Lancki DW, Moldwin RL, Dialynas DP, Fitch FW. 1988. J Immunol. 140(4):1034-1042. Jutila MA, Kroese FG, Jutila KL, Stall AM, Fiering S, Herzenberg LA, Berg EL, Butcher EC. 1988. Eur J Immunol. 18(11):1819-1826. Makaroff LE, Hendricks DW, Niec RE, Fink PJ. 2009. Proc Natl Acad Sci U S A. 106(12):4799-804.
Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.