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C57Bl/6 splenocytes were stimulated with PMA and Ionomycin (right panel) or unstimulated (left panel) and then stained with APC Anti-Mouse CD3e (20-0031), followed by intracellular staining with 0.125 ug FITC Anti-Mouse IFN gamma (35-7311).
  • C57Bl/6 splenocytes were stimulated with PMA and Ionomycin (right panel) or unstimulated (left panel) and then stained with APC Anti-Mouse CD3e (20-0031), followed by intracellular staining with 0.125 ug FITC Anti-Mouse IFN gamma (35-7311).

FITC Anti-Mouse IFN gamma (XMG1.2)

Cat No. Size Price Quantity
35-7311-U025 25 µg $30.00
35-7311-U100 100 µg $88.00

Description

The XMG1.2 antibody is specific for mouse Interferon-gamma (IFN-g), a 20 kDa type II cytokine known for its central roles in protection against bacterial or viral pathogens and for its anti-tumor properties. IFN-g is secreted by several types of immune cells which allow the cytokine to modulate innate immunity when secreted by NK and NKT cells, and to function in support of adaptive immunity when secreted by Th1 and CD8+ T cells (CTLs).

The XMG1.2 antibody is suitable for detection of intracellular IFN-g protein by flow cytometry. Other formats can be used for quantitative analysis of the secreted protein by ELISA when paired with an appropriate capture antibody. This clone has been reported for neutralization of the functional activity of ≥≥IFN-g in a variety of assays (use format suitable for functional assays).

Recent Publications:

Chen D, Duan Z, Zhou W, et al. Japanese encephalitis virus-primed CD8+ T cells prevent antibody-dependent enhancement of Zika virus pathogenesis. J Exp Med. 2020;217(9):e20192152. doi:10.1084/jem.20192152.

» View on PubMed

Wen J, Wang YT, Valentine KM, Dos Santos Alves RP, Xu Z, Regla-Nava JA, Ngono AE, Young MP, Ferreira LCS4, Shresta S. CD4+T Cells Cross-Reactive with Dengue and Zika Viruses Protect against Zika Virus Infection. Cell Rep. 2020 Apr 28;31(4):107566. doi: 10.1016/j.celrep.2020.107566.

» View on ScienceDirect

Blagih J, Zani F, Chakravarty P, et al. Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses. Cell Rep. 2020;30(2):481-496.e6. doi:10.1016/j.celrep.2019.12.028.

» View on PubMed

Derek W. Trobaugh, Chengqun Sun, Matthew D. Dunn, Douglas S. Reed, William B. Klimstra. Rational design of a live-attenuated eastern equine encephalitis virus vaccine through informed mutation of virulence determinants. PLoS Pathog. 2019 Feb 11;15(2):e1007584. doi: 10.1371/journal.ppat.1007584

» View on PubMed

Lam LKM, Watson AM, Ryman KD, Klimstra WB. Gamma-interferon exerts a critical early restriction on replication and dissemination of yellow fever virus vaccine strain 17D-204. NPJ Vaccines. 2018;3:5. Published 2018 Jan 23. doi:10.1038/s41541-017-0039-z.

» View on PubMed

Zellweger RM, Tang WW, Eddy WE, King K, Sanchez MC and Shresta S. 2015. J Virol. 89(12): 6494-6505. (Flow Cytometry)

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The XMG1.2 antibody is specific for mouse Interferon-gamma (IFN-g), a 20 kDa type II cytokine known for its central roles in protection against bacterial or viral pathogens and for its anti-tumor properties. IFN-g is secreted by several types of immune cells which allow the cytokine to modulate innate immunity when secreted by NK and NKT cells, and to function in support of adaptive immunity when secreted by Th1 and CD8+ T cells (CTLs).

The XMG1.2 antibody is suitable for detection of intracellular IFN-g protein by flow cytometry. Other formats can be used for quantitative analysis of the secreted protein by ELISA when paired with an appropriate capture antibody. This clone has been reported for neutralization of the functional activity of ≥≥IFN-g in a variety of assays (use format suitable for functional assays).

Recent Publications:

Chen D, Duan Z, Zhou W, et al. Japanese encephalitis virus-primed CD8+ T cells prevent antibody-dependent enhancement of Zika virus pathogenesis. J Exp Med. 2020;217(9):e20192152. doi:10.1084/jem.20192152.

» View on PubMed

Wen J, Wang YT, Valentine KM, Dos Santos Alves RP, Xu Z, Regla-Nava JA, Ngono AE, Young MP, Ferreira LCS4, Shresta S. CD4+T Cells Cross-Reactive with Dengue and Zika Viruses Protect against Zika Virus Infection. Cell Rep. 2020 Apr 28;31(4):107566. doi: 10.1016/j.celrep.2020.107566.

» View on ScienceDirect

Blagih J, Zani F, Chakravarty P, et al. Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses. Cell Rep. 2020;30(2):481-496.e6. doi:10.1016/j.celrep.2019.12.028.

» View on PubMed

Derek W. Trobaugh, Chengqun Sun, Matthew D. Dunn, Douglas S. Reed, William B. Klimstra. Rational design of a live-attenuated eastern equine encephalitis virus vaccine through informed mutation of virulence determinants. PLoS Pathog. 2019 Feb 11;15(2):e1007584. doi: 10.1371/journal.ppat.1007584

» View on PubMed

Lam LKM, Watson AM, Ryman KD, Klimstra WB. Gamma-interferon exerts a critical early restriction on replication and dissemination of yellow fever virus vaccine strain 17D-204. NPJ Vaccines. 2018;3:5. Published 2018 Jan 23. doi:10.1038/s41541-017-0039-z.

» View on PubMed

Zellweger RM, Tang WW, Eddy WE, King K, Sanchez MC and Shresta S. 2015. J Virol. 89(12): 6494-6505. (Flow Cytometry)

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Product Details

Name FITC Anti-Mouse IFN gamma (XMG1.2)
Cat. No. 35-7311
Alternative Names IFN-g, IFNg, Interferon-g, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Gene ID 15978
Clone XMG1.2
Isotype Rat IgG1, kappa
Reactivity Mouse
Cross Reactivity
Format FITC
Application Flow Cytometry
Citations*

Chen D, Duan Z, Zhou W, et al. Japanese encephalitis virus-primed CD8+ T cells prevent antibody-dependent enhancement of Zika virus pathogenesis. J Exp Med. 2020;217(9):e20192152. doi:10.1084/jem.20192152.

» View on PubMed

Wen J, Wang YT, Valentine KM, Dos Santos Alves RP, Xu Z, Regla-Nava JA, Ngono AE, Young MP, Ferreira LCS4, Shresta S. CD4+T Cells Cross-Reactive with Dengue and Zika Viruses Protect against Zika Virus Infection. Cell Rep. 2020 Apr 28;31(4):107566. doi: 10.1016/j.celrep.2020.107566.

» View on ScienceDirect

Blagih J, Zani F, Chakravarty P, et al. Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses. Cell Rep. 2020;30(2):481-496.e6. doi:10.1016/j.celrep.2019.12.028.

» View on PubMed

Derek W. Trobaugh, Chengqun Sun, Matthew D. Dunn, Douglas S. Reed, William B. Klimstra. Rational design of a live-attenuated eastern equine encephalitis virus vaccine through informed mutation of virulence determinants. PLoS Pathog. 2019 Feb 11;15(2):e1007584. doi: 10.1371/journal.ppat.1007584

» View on PubMed

Lam LKM, Watson AM, Ryman KD, Klimstra WB. Gamma-interferon exerts a critical early restriction on replication and dissemination of yellow fever virus vaccine strain 17D-204. NPJ Vaccines. 2018;3:5. Published 2018 Jan 23. doi:10.1038/s41541-017-0039-z.

» View on PubMed

Choudhry N, Petry F, van Rooijen N, and McDonald V. 2012. J. of Infect. Disease. 206: 117-124. (in vivo neutralization)

Cobb D and Smeltz RB. 2012. J. Immunol. 188: 3766-3773. (in vitro neutralization)

Brown DM, Lee S, Garcia-Hernandez M, and Swain SL. 2012. J. Virol. 86: 6792-6803. (ELISpot - detection)

Yu H, Karunakaran KP, Jiang X, Shen C, Andersen P, and Brunham RC. 2012. Infect. Immun. 80: 1510-1518. (ELISpot -detection)

Kwon M-J, Ma J, Ding Y, Wang R, and Sun Z. 2012. J. Immunol. 188: 5887-5897. (in vitro induction of Th2 polarization)

Barr TA, Shen P, Brown S, Lampropoulou V, Roch T, Lawrie S, Fan B, O’Connor RA, Anderton SM, Bar-Or Am Fillatreau S, and Gray D. 2012. J. Exp. Med. 209: 1001-1010. (Flow cytometry)

Cardona AE, Restrepo BI, Jaramillo JM, and Teale JM. 1999. J. Immunol. 162: 995-1002. (Immunohistochemistry – frozen tissue)

Kupfer A, Mosmann TR, and Kupfer H. 1991. Proc. Natl. Acad. Sci. 88: 775-779. (Immunofluorescence microscopy)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.