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C57Bl/6 (left panel) or SJL (right panel) splenocytes were stained with 0.125 ug FITC Anti-Mouse CD45.1 (35-0453) (solid line) or 0.125 ug FITC Mouse IgG2a isotype control (dashed line).
  • C57Bl/6 (left panel) or SJL (right panel) splenocytes were stained with 0.125 ug FITC Anti-Mouse CD45.1 (35-0453) (solid line) or 0.125 ug FITC Mouse IgG2a isotype control (dashed line).
  • FITC Anti-Mouse CD45.1 (A20)

FITC Anti-Mouse CD45.1 (A20)

Cat No. Size Price Quantity
35-0453-U025 25 µg $30.00
35-0453-U100 100 µg $79.00
35-0453-U500 500 µg $100.00

Description

The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.

The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

 

Recent Publications:

Nishina T, Deguchi Y, Ohshima D, et a. Interleukin-11-expressing fibroblasts have a unique gene signature correlated with poor prognosis of colorectal cancer. Nat Commun. 2021 Apr 16;12(1):2281. doi: 10.1038/s41467-021-22450-3. PMID: 33863879.

» View on Nature Communications 

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The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.

The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

 

Recent Publications:

Nishina T, Deguchi Y, Ohshima D, et a. Interleukin-11-expressing fibroblasts have a unique gene signature correlated with poor prognosis of colorectal cancer. Nat Commun. 2021 Apr 16;12(1):2281. doi: 10.1038/s41467-021-22450-3. PMID: 33863879.

» View on Nature Communications 

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Product Details

Name FITC Anti-Mouse CD45.1 (A20)
Cat. No. 35-0453
Alternative Names SJL, Ly5.1
Gene ID 19264
Clone A20
Isotype Mouse IgG2a, κ
Reactivity Mouse
Cross Reactivity
Format FITC
Application Flow Cytometry
Citations*

Scharer CD, Patterson DG, Mi T, Price MJ, Hicks SL, Boss JM. Antibody-secreting cell destiny emerges during the initial stages of B-cell activation. Nat Commun. 2020;11(1):3989. Published 2020 Aug 10. doi:10.1038/s41467-020-17798-x.

» View on Nature Communications

Guo M, Price MJ, Patterson DG, et al. EZH2 Represses the B Cell Transcriptional Program and Regulates Antibody-Secreting Cell Metabolism and Antibody Production. J Immunol. 2018;200(3):1039-1052. doi:10.4049/jimmunol.1701470.

» View on PubMed

Willinger T and Flavell RA. 2012. Proc. Natl. Acad. Sci. 109: 8670-8675. (Flow Cytometry)

Siggs OM, Li X, Xia Y, and Beutler B. 2012. J. Exp. Med. 209:19-27. (Flow Cytometry)

Wakim LM, Woodward-Davis A and Bevan MJ. 2010. 107: 17872-17879. (Immunohistochemistry – OCT embedded frozen tissue)

Ato M, Nakano H, Kakiuchi T, and Kaye PM. 2004. J. Immunol. 173: 4815-4820. (Immunohistochemistry – frozen tissue)

Shen F-W, Tung J-S and Boyse EA. 1986. Immunogenetics. 24(3): 146-149. (Immunoprecipitation)

Application Key:

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.