FITC Anti-Human/Mouse CD11b (M1/70)
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 35-0112-U025 | 25 µg | $24.00 | ||
| 35-0112-U100 | 100 µg | $64.00 | ||
| 35-0112-U500 | 500 µg | $115.00 |
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The M1/70 antibody reacts with human and mouse CD11b, also known as integrin αalpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin αreceptors that mediate adhesion between ≥ ≥ ≥ cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrin αs are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin αreceptors consist of distinct combinations of integrin αchains which are differentially expressed. integrin αalpha M (CD11b) assembles with integrin αbeta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.
The M1/70 antibody is widely used as a marker for CD11b expression on mouse macrophages, granulocytes, neutrophils, and NK cells. The antibody is also reported to be cross-reactive for Rhesus macaque CD11b.
Recent Publications:
Baba T, Yoshida T, Tanabe Y, et al. Cytoplasmic DNA accumulation preferentially triggers cell death of myeloid leukemia cells by interacting with intracellular DNA sensing pathway. Cell Death Dis. 2021 Mar 26;12(4):322. doi: 10.1038/s41419-021-03587-x. PMID: 33771977.
Li Y, Han Y, Su R, Liu Y, Chong G, Xu D, He R, Liu Y, Dong H, Shi D, Li Y. Photosensitizer-Laden Neutrophils Are Controlled Remotely for Cancer Immunotherapy. Cell Rep. 2020 Dec 15;33(11):108499. doi: 10.1016/j.celrep.2020.108499. PMID: 33326787.
Bescucci DM, Clarke ST, Brown CLJ, et al. The absence of murine cathelicidin-related antimicrobial peptide impacts host responses enhancing Salmonella enterica serovar Typhimurium infection. Gut Pathog. 2020 Nov 13;12(1):53. doi: 10.1186/s13099-020-00386-1. PMID: 33292444; PMCID: PMC7666523.
Komura N, Mabuchi S, Shimura K, et al. The role of myeloid-derived suppressor cells in increasing cancer stem-like cells and promoting PD-L1 expression in epithelial ovarian cancer. 2020 Jun 19. Cancer Immunol Immunother. 2020;10.1007/s00262-020-02628-2. doi:10.1007/s00262-020-02628-2.
Markman, J.L., Porritt, R.A., Wakita, D. et al. Loss of testosterone impairs anti-tumor neutrophil function. Nat Commun 11, 1613 (2020). https://doi.org/10.1038/s41467-020-15397-4.
» View on Nature Communications
Shiyang Li, John W. Bostick, Jian Ye, Joseph F. Urban, Jr., Dorina Avram, Liang Zhou. Aryl Hydrocarbon Receptor Signaling Cell Intrinsically Inhibits Intestinal Group 2 Innate Lymphoid Cell Function. Immunity. 2018 Nov 20;49(5):915-928.e5. doi: 10.1016/j.immuni.2018.09.015
Palacios M, Miner TA, Frederick DR, et al. Identification of Two Regulators of Virulence That Are Conserved in Klebsiella pneumoniae Classical and Hypervirulent Strains. mBio. 2018;9(4):e01443-18. Published 2018 Aug 7. doi:10.1128/mBio.01443-18.
Scharer CD, Barwick BG, Guo M, Bally APR, Boss JM. Plasma cell differentiation is controlled by multiple cell division-coupled epigenetic programs. Nat Commun. 2018;9(1):1698. Published 2018 Apr 27. doi:10.1038/s41467-018-04125-8.
Kawano M, Mabuchi S, Matsumoto Y, Sasano T et al. 2015. Sci Rep. 5:18217 doi:10.1038/srep18217. (Flow Cytometry)
The M1/70 antibody reacts with human and mouse CD11b, also known as integrin αalpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin αreceptors that mediate adhesion between ≥ ≥ ≥ cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrin αs are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin αreceptors consist of distinct combinations of integrin αchains which are differentially expressed. integrin αalpha M (CD11b) assembles with integrin αbeta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.
The M1/70 antibody is widely used as a marker for CD11b expression on mouse macrophages, granulocytes, neutrophils, and NK cells. The antibody is also reported to be cross-reactive for Rhesus macaque CD11b.
Recent Publications:
Baba T, Yoshida T, Tanabe Y, et al. Cytoplasmic DNA accumulation preferentially triggers cell death of myeloid leukemia cells by interacting with intracellular DNA sensing pathway. Cell Death Dis. 2021 Mar 26;12(4):322. doi: 10.1038/s41419-021-03587-x. PMID: 33771977.
Li Y, Han Y, Su R, Liu Y, Chong G, Xu D, He R, Liu Y, Dong H, Shi D, Li Y. Photosensitizer-Laden Neutrophils Are Controlled Remotely for Cancer Immunotherapy. Cell Rep. 2020 Dec 15;33(11):108499. doi: 10.1016/j.celrep.2020.108499. PMID: 33326787.
Bescucci DM, Clarke ST, Brown CLJ, et al. The absence of murine cathelicidin-related antimicrobial peptide impacts host responses enhancing Salmonella enterica serovar Typhimurium infection. Gut Pathog. 2020 Nov 13;12(1):53. doi: 10.1186/s13099-020-00386-1. PMID: 33292444; PMCID: PMC7666523.
Komura N, Mabuchi S, Shimura K, et al. The role of myeloid-derived suppressor cells in increasing cancer stem-like cells and promoting PD-L1 expression in epithelial ovarian cancer. 2020 Jun 19. Cancer Immunol Immunother. 2020;10.1007/s00262-020-02628-2. doi:10.1007/s00262-020-02628-2.
Markman, J.L., Porritt, R.A., Wakita, D. et al. Loss of testosterone impairs anti-tumor neutrophil function. Nat Commun 11, 1613 (2020). https://doi.org/10.1038/s41467-020-15397-4.
» View on Nature Communications
Shiyang Li, John W. Bostick, Jian Ye, Joseph F. Urban, Jr., Dorina Avram, Liang Zhou. Aryl Hydrocarbon Receptor Signaling Cell Intrinsically Inhibits Intestinal Group 2 Innate Lymphoid Cell Function. Immunity. 2018 Nov 20;49(5):915-928.e5. doi: 10.1016/j.immuni.2018.09.015
Palacios M, Miner TA, Frederick DR, et al. Identification of Two Regulators of Virulence That Are Conserved in Klebsiella pneumoniae Classical and Hypervirulent Strains. mBio. 2018;9(4):e01443-18. Published 2018 Aug 7. doi:10.1128/mBio.01443-18.
Scharer CD, Barwick BG, Guo M, Bally APR, Boss JM. Plasma cell differentiation is controlled by multiple cell division-coupled epigenetic programs. Nat Commun. 2018;9(1):1698. Published 2018 Apr 27. doi:10.1038/s41467-018-04125-8.
Kawano M, Mabuchi S, Matsumoto Y, Sasano T et al. 2015. Sci Rep. 5:18217 doi:10.1038/srep18217. (Flow Cytometry)
Product Details
| Name | FITC Anti-Human/Mouse CD11b (M1/70) |
|---|---|
| Cat. No. | 35-0112 |
| Alternative Names | Mac-1, integrin α?M, CR3, ITGAM |
| Gene ID | 16409 / 3684 |
| Clone | M1/70 |
| Isotype | Rat IgG2b, κ |
| Reactivity | Human, Mouse |
| Cross Reactivity | Chimpanzee, Baboon, Cynomolgus, Rhesus |
| Format | FITC |
| Application | Flow Cytometry |
| Citations* |
Komura N, Mabuchi S, Shimura K, et al. The role of myeloid-derived suppressor cells in increasing cancer stem-like cells and promoting PD-L1 expression in epithelial ovarian cancer. 2020 Jun 19. Cancer Immunol Immunother. 2020;10.1007/s00262-020-02628-2. doi:10.1007/s00262-020-02628-2. Shiyang Li, John W. Bostick, Jian Ye, Joseph F. Urban, Jr., Dorina Avram, Liang Zhou. Aryl Hydrocarbon Receptor Signaling Cell Intrinsically Inhibits Intestinal Group 2 Innate Lymphoid Cell Function. Immunity. 2018 Nov 20;49(5):915-928.e5. doi: 10.1016/j.immuni.2018.09.015 Palacios M, Miner TA, Frederick DR, et al. Identification of Two Regulators of Virulence That Are Conserved in Klebsiella pneumoniae Classical and Hypervirulent Strains. mBio. 2018;9(4):e01443-18. Published 2018 Aug 7. doi:10.1128/mBio.01443-18. Scharer CD, Barwick BG, Guo M, Bally APR, Boss JM. Plasma cell differentiation is controlled by multiple cell division-coupled epigenetic programs. Nat Commun. 2018;9(1):1698. Published 2018 Apr 27. doi:10.1038/s41467-018-04125-8. Lefort CT, Rossaint J, Moser M, Petrich BG, Zarbock A, Monkley SJ, Critchley DR, Ginsberg MH, Fassler R, and Ley K. 2012. Blood. 119:4275-4282. (in vitro blocking) Grewal JS, Pilgrim MJ, Grewal S, Kasman L, Werner P, Bruorton ME, London SD, and London L. 2011. FASEB J. 25:1680-1696. (Immunofluorescence microscopy – frozen tissue) Kim W-K, Sun Y, Do H, Autissier P, Halpern EF, Piatak M, Lifson JD, Burdo TH, McGrath MS, and Williams K. 2010. J. Leukoc. Biol. 87: 557-567. (Flow Cytometry – Rhesus macaque) Roland CL, Dineen SP, Lynn KD, Sullivan LA, Dellinger MT, Sadegh L, Sullivan JP, Shames DS, and Brekken RA. 2009. Mol. Cancer Ther. 8:1761-1771. (Immunofluorescence microscopy – frozen tissue) Sorg H, Lorch B, Jaster R, Fitzner B, Ibrahim S, Holzhueter S, Nizze H, and Vollmar B. 2008. Am. J. Physio. Gastrointest. Liver Physiol. 295: G1274-1280. (Immunohistochemistry - formalin-fixed paraffin embedded tissue) Kim DD, Miwa T, Kimura Y, Schwendener RA, van Lookeren Campagne M, and Song W-C. 2008. Blood. 112:1109-1119. (in vivo blocking) Ou R, Zhang M, Huang L, Flavell RA, Koni PA, and Moskophidis D. 2008. J. Virol. 82:2952-2965. (Immunohistochemistry – OCT embedded frozen tissue) Nutt SL, Metcalf D, D’Amico A, Polli M, and Wu L. 2005. J. Exp. Med. 201:221-231. (Immunomagnetic bead depletion) Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, and Hill TJ. 1995. J. Histochem. Cytochem. 43:313-320. (Immunohistochemistry – frozen tissue, paraffin embedded tissue) Miller LJ, Schwarting R, and Springer TA. 1986. J. Immunol. 137:2891-2900. (Immunoprecipitation) |
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.