FITC Annexin V
| Cat No. | Size | Price | Quantity | |
|---|---|---|---|---|
| 35-6409-T025 | 25 tests | $60.00 | ||
| 35-6409-T100 | 100 tests | $155.00 |
Technical Data Sheet
Request Custom/BulkDescription
Annexin V belongs to the annexin family of calcium-dependent phospholipid binding proteins and has a high affinity for phosphatidylserine (PS). One of the characteristic features of early stage apoptosis is the translocation of PS from the inner to the outer leaflet of the plasma membrane, exposing it to the extracellular environment where it can be detected. Fluorochrome conjugated Annexin V can serve as a sensitive detection probe for this hallmark of early stage apoptotic change.
Annexin V is generally used in combination with a viability dye, such as 7-AAD or propidium iodide (PI), that cannot pass through intact cellular membranes. Translocation of PS occurs prior to loss of membrane integrity, therefore viability dyes would be excluded from the cell. As cells progress through the apoptotic process, membranes are compromised and the viability dyes are able to enter the cell. Using this method, cells that are Annexin V positive and viability dye negative are in early apoptosis. If cells are positive for both Annexin V and the viability dye, they are either undergoing apoptotic death or necrosis. When negative for both markers, cells are viable.
Recent Citations:
Gergen AK, Madsen HJ, Li A, Cheng L, Meng X, Fullerton DA, Pratap A, Weyant MJ. Simvastatin Inhibits Histologic Changes Associated with Gastroduodenal Reflux in a Murine Model. Dig Dis Sci. 2022 Jan 10. doi: 10.1007/s10620-021-07344-0. Epub ahead of print. PMID: 35001242.
Annexin V belongs to the annexin family of calcium-dependent phospholipid binding proteins and has a high affinity for phosphatidylserine (PS). One of the characteristic features of early stage apoptosis is the translocation of PS from the inner to the outer leaflet of the plasma membrane, exposing it to the extracellular environment where it can be detected. Fluorochrome conjugated Annexin V can serve as a sensitive detection probe for this hallmark of early stage apoptotic change.
Annexin V is generally used in combination with a viability dye, such as 7-AAD or propidium iodide (PI), that cannot pass through intact cellular membranes. Translocation of PS occurs prior to loss of membrane integrity, therefore viability dyes would be excluded from the cell. As cells progress through the apoptotic process, membranes are compromised and the viability dyes are able to enter the cell. Using this method, cells that are Annexin V positive and viability dye negative are in early apoptosis. If cells are positive for both Annexin V and the viability dye, they are either undergoing apoptotic death or necrosis. When negative for both markers, cells are viable.
Recent Citations:
Gergen AK, Madsen HJ, Li A, Cheng L, Meng X, Fullerton DA, Pratap A, Weyant MJ. Simvastatin Inhibits Histologic Changes Associated with Gastroduodenal Reflux in a Murine Model. Dig Dis Sci. 2022 Jan 10. doi: 10.1007/s10620-021-07344-0. Epub ahead of print. PMID: 35001242.
Product Details
| Name | FITC Annexin V |
|---|---|
| Cat. No. | 35-6409 |
| Protocol | Download Protocol |
| Alternative Names | |
| Format | FITC |
| Application | Flow Cytometry |
| Citations* |
Koopman G, Reutelingsperger CP, Kuijten GA, Keehnen RM, Pals ST, van Oers MH. 1994. Blood. 84(5):1415-1420. Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C. 1995. J. Immunol. Methods. 184(1):39-51. |
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.